A Cytotaxonomic Study in Twelve Brazilian Taxa of Stylosanthes Sw., Leguminosae.

Vieira, Maria Lúcia Carneiro; Aguiar-Perecin, Margarida Lopes Rodrigues de; Martins, Paulo Sodero

doi:10.1508/cytologia.58.305

Cited by 13 publications

(19 citation statements)

References 12 publications

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“…Each species has rich variations in morphology, physiology, and genetics. S. guianensis is the most widespread Stylosanthes species and exhibits remarkable phenotypic variability (Williams et al 1984;Vieira et al 1993). This species is one of the most important tropical forage legumes currently known and is native to South and Central America and Africa, where it is widely distributed.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species

2017

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A total of 148 accessions representing six important species of the genus Stylosanthes, including S. guianensis, S. hamata, S. scabra, S. seabrana, S. macrocephala, and S. capitata, were used to evaluate genetic variation and relationships using sequence-related amplified polymorphism markers. The results showed that the 18 selected primer pairs generated 138 distinct fragments. The fragment sizes ranged from 150 to 2000 bp. Genetic similarity coefficients among the 148 accessions ranged from 0.51 to 0.99, with an average of 0.79. The effective allele number (ne) generated by the 18 primer pairs averaged 1.3552 and ranged from 1.2069 to 1.6080; Nei's gene diversity (He) ranged from 0.1304 to 0.3207, with an average of 0.2070; and Shannon's information index (I) averaged 0.3213 and ranged from 0.2233 to 0.4582. The unweighted pair-group method with arithmetic averages at the 0.69 similarity level separated the 148 accessions into two distinct groups. One group belonged to S. guianensis, and the other group belonged to the non-S. guianensis type. This study verified that Stylosanthes have rich genetic variation, which is an excellent basis for Stylosanthes breeding for new cultivars. This study demonstrates that the SRAP technique is a reliable tool for differentiating Stylosanthes accessions and for discerning genetic relationship among them.

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Section: Introductionmentioning

confidence: 99%

Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species

2017

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“…Given the difficulties in species delimitation and the significance of Stylosanthes in (sub)tropical agriculture, other techniques have been used in an attempt to complement the morphological approach (e.g. Williams et al 1984;Stace and Cameron 1987;Vieira et al 1993). Recently, molecular marker techniques have proven to be very useful in providing additional data for delineating species (Liu et al 1999;Vander Stappen et al 1999b), assessing evolutionary relationships and elucidating the genetic origin of allopolyploids (Gillies and Abbott 1996;Vander Stappen et al 1999a;Liu and Musial 2001;Vander Stappen et al 2002a, b), and determining genetic diversity (Vander Stappen et al 1998Sawkins et al 2001) in the genus Stylosanthes.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae)

2003

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Supplementary Material The nucleotide sequence of the ribosomal external transcribed spacer (ETS) region of Stylosanthes mexicana was determined and used to evaluate its potential for examination of intra- and inter-specific relationships in Stylosanthes, as compared to the use of the internal transcribed spacer (ITS) region. The entire ETS region comprises 1,145 bp and is composed of a region of non-repetitive sequences consisting of three subregions with organizational and nucleotide-sequence conservation, and a triplicated segment of about 100 bp. A primer designed in the second conserved subregion allowed us to amplify and sequence directly the 3' part (423-431 bp) of the ETS from 22 genotypes of 12 representative Stylosanthes species that were previously used in phylogenetic analysis of the genus. The study revealed that the right-hand part of Stylosanthes ETS contains approximately twice as much variable and informative characters than the ITS. Moreover, pairwise sequence-divergence values are twice as high, on average, when compared to the ITS. The ITS and ETS datasets are consistent in phylogenetic reconstruction of Stylosanthes, and combined parsimony analysis resulted in a strict consensus tree that is better resolved and generally better supported than trees obtained from separate analysis of the spacer regions.

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“…Our group has used this strategy to determine the karyotype of Stylosanthes (VIEIRA et al, 1993) and Passiflora species (CUCO et al, 2005) as well as for Crotalaria juncea (MONDIN et al, 2007). For this reason, we may speculate here that the variation observed in chromosome 2 is due to a shift of the centromere segment.…”

Section: Discussionmentioning

confidence: 99%

Karyotype characterization of Malpighia emarginata (Malpighiaceae)

2010

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-Malpighia emarginata Sessé & Mociño ex DC. or West Indian cherry (acerola) is a wild plant originated in southern Mexico, Central America and the northern region of South America. The species was introduced to Brazil about 60 years ago and now the country is the world's biggest producer. Even though the fruits of acerola have high commercial value, as they are an important source of the natural vitamin C, very little chromosome information is available for this species. Previous studies showed that most Malpighia species are diploids, including M. emarginata with 2n = 20. In the present paper, the chromosome number of acerola was confirmed, and for the first time, its karyotype was described, providing the identification of the homologues for the ideogram construction. The acerola chromosomes are small (1.71 to 2.56 µm) and metacentric with the exception of chromosome 2 that was classified as submetacentric. In addition, it is recommended a protocol to produce rooted-plantlets in vitro for mitotic studies that could be also used for micropropagation of acerola. Index terms: acerola, cytology, chromosomes, in vitro culture establishment, West Indian cherry. CARACTERIZAÇÃO CARIOTÍPICA DE Malpighia emarginata (MALPIGHIACEAE)RESUMO -Malpighia emarginata Sessé e Mociño ex DC., ou acerola, é uma planta silvestre originada do sul do México, América Central e região norte da América do Sul. A espécie foi introduzida no Brasil há cerca de 60 anos e o País é o maior produtor mundial. Embora os frutos da acerola tenham alto valor comercial, pois são uma importante fonte natural de vitamina C, pouco se conhece sobre os cromossomos da espécie. Estudos prévios mostraram que a maioria das espécies de Malpighia é diploide, incluindo M. emarginata com 2n = 20. No presente trabalho, foi confirmado o número cromossômico da acerola e foi descrito, pela primeira vez, o seu cariótipo, provendo a identificação dos homólogos para a construção do ideograma. Os cromossomos da acerola são pequenos (1,71 a 2,56 µm) e metacêntricos, com exceção do cromossomo 2, que foi classificado como submetacêntrico. Além disso, definiu-se um protocolo para produzir plântulas enraizadas in vitro para estudos mitóticos, o qual pode, igualmente, ser usado para a micropropagação da acerola. Termos para indexação: acerola, citologia, cromossomos, estabelecimento in vitro, West Indian cherry.

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A Cytotaxonomic Study in Twelve Brazilian Taxa of Stylosanthes Sw., Leguminosae.

Cited by 13 publications

References 12 publications

Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species

Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species

Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae)

Karyotype characterization of Malpighia emarginata (Malpighiaceae)

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