A cytological study of the diurnal cycle of the liver of the mouse in relation to storage and secretion

Abstract

The correlation between changes in the cell organelles and the processes of storage and secretion is one of the fundamental problems of cytology. I n the liver many of the activities of the cell are known through biochemical studies, and in certain instances the products of these activities-e.g. bile acids, glycogen, and fat-can be demonstrated histologically. Several suggestions have appeared in the literature of interrelationships between the organelles and the processes associated with alimentation, but no comprehensive study has been undertaken. The present investigation is such a study of the relationship between changes in the mitochondria and the Golgi substance on the one hand and the secretion of bile acids and the storage of glycogen and fat on the other.Recently Forsgren ( '35) and his associates have described reciprocal diurnal cycles in the deposition of glycogen and in the secretion of the bile components (actually the bile acids, because of the method of demonstration). According to their observations the greatest amount of glycogen is present in the liver during the night, whereas the largest amount of bile acid material is secreted during the afternoon. Except ' A portion of a thesis submitted in partial fulfillment of the requirements for a Horton-Hallowell Fellow of Wellesley College, 1942-43. the degree of Doctor

Electron Microscopic Radioautographic Detection of Sites of Protein Synthesis and Migration in Liver

“…6) . The rationale behind the exclusion of the glycogen area is that cell size (11) and liver weight (47) increase with increasing glycogen content in liver cells, the accumulated glycogen apparently causing expansion of the cytoplasmic volume rather than reduction in amounts of the constituents already present. QUANTITATION ; SILVER GRAINS : Figs .…”

Effects of compound 48/80 on hepatic glycogen and glucose‐6‐phosphatase early in the diurnal cycle of the rat

“…This type of loss was described by a number of investigators (e.g., Smith, 1931;Deane, 1944;Deane et al, 1946;Corrin and Aterman, 1968). Deane (1944) attributed the depletion of glycogen from the centrilobular zone to anoxia that, according to Matthews (1939), stimulates the immediate breakdown of glycogen to sugar under anaerobic conditions. Compound 48/80, when injected intravenously into the rat or applied to the surface of the liver, has been shown to produce, respectively, a decrease in systemic blood pressure or a decrease in intrahepatic blood flow (Reilly et al, , 1983b.…”

Fine structural alterations induced by cortisol administration in non-adrenalectomized/non-fasted rat hepatocytes

“…Currently, although their effects on hepatic histology have been adequately studied, the alterations in ultrastructure of hepatocytes remain partially elucidated. As reported above, our current knowledge mainly relies on observations in adrenalectomized or fasted animals and concerns changes in the morphology of the endoplasmic reticulum and glycogen deposits [1][2][3][4][5]. Nevertheless, the impact of exogenously administered corticosteroids in nonadrenalectomized and fed animals might be of particular interest because the induced ultrastructural changes in bintactQ animals may affect liver cell functionality in a different way.…”

“…The bcontrolled 1567-5769/$ -see front matter D 2004 Elsevier B.V. All rights reserved. doi:10.1016/j.intimp.2004.09.004 feeding cycleQ introduced by Dean in early 1940s, has served as an experimental model of fasting for known periods of time, but its reliability is unproven since it cannot perfectly parallel the natural feeding habits of the rodents [3,4]. An alternative experimental model of endogenous cortisol depletion by surgical bilateral adrenalectomy has been developed in order to provide more bunbiasedQ information about the effect of cortisol on liver parenchyma, but to our opinion, this model induces an important surgical stress unavoidably implicating liver and possibly leading to conflicting results [5].…”