Fine structural alterations induced by cortisol administration in non-adrenalectomized/non-fasted rat hepatocytes

Pilichos, Charalampos; Preza, A.; Kounavis, I.; Zafeiratou, S.; Kapatsoris, D.

doi:10.1016/j.intimp.2004.09.004

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…With regards to protein synthesis in mitochondria in animal cells or plastids in plant cells, many studies have recently reported various cells of some plants [66,67,68] or several kinds of animals, such as hamster [69], catfish [70], rat [71], or human patients [72,73,74] by various approaches. Most of these authors observed mitochondrial protein synthesis in various cells by means of biochemical procedures.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice

Nagata

2006

The Scientific World JOURNAL

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For the purpose of studying the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of 3 individuals (total 30), from fetal day 19 to postnatal month 24, were injected during development with 3H-leucine, a protein precursor, sacrificed 1 h later, and the liver tissues processed for electron microscopic (EM) radioautography. On EM radioautograms obtained from each animal, the number of mitochondria, the number of labeled mitochondria, and the mitochondrial labeling index labeled with silver grains due to H-leucine showing protein synthesis in each mononucleate hepatocytes were counted and the averages in respective aging groups were compared. From the results, it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein syntheses in mononucleate hepatocytes of mice at various ages from embryonic day 19 to postnatal month 24 increased and decreased due to development and aging of animals.

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Section: Discussionmentioning

confidence: 99%

Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice

Nagata

2006

The Scientific World JOURNAL

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“…A stimulating effect of Cort on the proliferation of endoplasmic reticulum in stage I and II oocytes, as observed in our data, was not described yet in other studies with oocytes. However, in the thymus of chicken embryo (Chan 1991) and in rat hepatocytes, the endoplasmic reticulum was more developed and increased in size and complexity in response to Cort (Chan 1991, Pilichos et al 2005. Further investigations are needed to analyze whether the increase of endoplasmic reticulum by Cort may be a sign of stimulated early oogenesis.…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular effects of cortisol and ACTH on zebrafish stage I and II follicles

et al. 2015

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Oogenesis in zebrafish (Danio rerio) is controlled by the hypothalamus-pituitary-gonadal axis and reproductive hormones. In addition, an interference of stress hormones is known with reproductive biology. In the presented work, we aimed to explore the hypothesis that cortisol (Cort) and ACTH may affect early oogenesis in zebrafish, given the presence of the specific receptors for glucocorticoids and ACTH in the zebrafish ovary. Follicles at stages I and II were exposed in vitro to 1 mM Cort and ACTH for 48 h, then ultrastructural and molecular effects were analyzed. The comet assay demonstrated increased tail moments for Cort and ACTH treatment indicative of DNA damage. The mRNA expression of apoptotic genes (bax, bcl-2) was not altered by both treatments, but Cort increased significantly the expression of the ACTH receptor (mc2r). Cort stimulated the presence of the endoplasmic reticulum, predominantly at stage II, while ACTH induced a strong vacuolization. Viability of oocytes was not affected by both treatments and fluorescent staining (monodansylcadaverine/acridine orange) indicated a reduced quantity of autophagosomes for ACTH, and lower presence of nucleic acids in ooplasm for Cort and ACTH. Concluding, different responses were observed for stress hormones on early stages of zebrafish oocytes, which suggest a role for both hormones in the stress-mediated adverse effects on female gametogenesis. Reproduction (2015) 150 429-436

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“…Concerning the protein synthesis in mitochondria in animal cells or plastids in plant cells, it has been observed in various cells of some plants [53,54,55], several kinds of animals (such as catfish [56], hamster [57] and rat [58]), or human patients [59,60,61], by means of various approaches. Most of these authors observed mitochondrial protein synthesis in various cells by biochemical procedures.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopic Radioautographic Study on Protein Synthesis in Mitochondria of Binucleate Hepatocytes of Aging Mice

Nagata

2007

The Scientific World JOURNAL

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In order to study the aging changes of intramitochondrial protein synthesis in mouse hepatocytes, 10 groups of aging mice, each consisting of three individuals, total 30, from fetal day 19 to postnatal year 2, were injected with 3H-leucine, a protein precursor, sacrificed 1 h later, and the liver tissues processed for electron microscopic radioautography. On electron microscopic radioautograms obtained from each animal, the numbers of mitochondria, the numbers of labeled mitochondria, and the mitochondrial labeling index labeled with 3H-leucine that showed protein synthesis in each hepatocyte, both mononucleate and binucleate cells, were counted and the averages in respective aging groups were compared. From the results, it was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein syntheses in both mononucleate and binucleate hepatocytes of mice at various ages increased due to development of animals. The numbers of mitochondria, the numbers of labeled mitochondria, and the labeling indices of intramitochondrial protein synthesis in binucleate hepatocytes were more than those of mononucleate hepatocytes at the same aging stages.

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Fine structural alterations induced by cortisol administration in non-adrenalectomized/non-fasted rat hepatocytes

Cited by 3 publications

References 11 publications

Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice

Electron Microscopic Radioautographic Study on Protein Synthesis in Hepatocyte Mitochondria of Aging Mice

Morphological and molecular effects of cortisol and ACTH on zebrafish stage I and II follicles

Electron Microscopic Radioautographic Study on Protein Synthesis in Mitochondria of Binucleate Hepatocytes of Aging Mice

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