A customized multispectral needle probe combined with a virtual photometric setup for in vivo detection of Lewis lung carcinoma in an animal model

Braun, Frank; Schalk, Robert; Nachtmann, Marcel; Hien, Andreas; Frank, Ronald; Beuermann, Thomas; Methner, Frank‐Jürgen; Kränzlin, Bettina; Rädle, Matthias; Gretz, Norbert

doi:10.1088/1361-6501/ab24a1

Cited by 8 publications

(12 citation statements)

References 52 publications

(60 reference statements)

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“…All samples can be assigned to their respective groups. Classical histology validates all measurements [13].…”

Section: Combination Techniques: Uv/vis/nir/fluorescencesupporting

confidence: 70%

“…Furthermore, probes for scattered-light measurements can be extremely small. For example, manufacturing backscatter probes even in a blunt cannula with an inner diameter of 1.1 mm is possible [13].…”

Section: Particle Detectionmentioning

confidence: 99%

“…In such a blunt cannula ( Figure 5), several glass fibers can be included for different light sources and detectors. This technology is used for measurements in micro channels to obtain particle size, concentration, and growth [13]. Several limitations may apply, but particle size can, in most cases, be determined with an acquisition rate of over 1000 Hz.…”

Section: Particle Detectionmentioning

confidence: 99%

“…The basic setup, presented in Figure 13, contains glass fibers for ultraviolet, visible, near infrared and fluorescence spectroscopy. The probe head consists of a name-giving needle and seven bare glass fibers for illumination and detection [13]. Glass fibers can be used to transport light from the probe head to the spectrometer or detection unit [29,30].…”

Section: Combination Techniques: Uv/vis/nir/fluorescencementioning

confidence: 99%

“…Ultraviolet (UV), visible (VIS), NIR, and fluorescence measurements are possible. The needle is optimized for tissue penetration with a front and back cut[13].…”

mentioning

confidence: 99%

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Molecule Sensitive Optical Imaging and Monitoring Techniques—A Review of Applications in Micro-Process Engineering

2020

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This paper provides an overview of how molecule-sensitive, spatially-resolved technologies can be applied for monitoring and measuring in microchannels. The principles of elastic light scattering, fluorescence, near-infrared, mid-infrared, and Raman imaging, as well as combination techniques, are briefly presented, and their advantages and disadvantages are explained. With optical methods, images can be acquired both scanning and simultaneously as a complete image. Scanning technologies require more acquisition time, and fast moving processes are not easily observable. On the other hand, molecular selectivity is very high, especially in Raman and mid-infrared (MIR) scanning. For near-infrared (NIR) images, the entire measuring range can be simultaneously recorded with indium gallium arsenide (InGaAs) cameras. However, in this wavelength range, water is the dominant molecule, so it is sometimes necessary to use complex learning algorithms that increase the preparation effort before the actual measurement. These technologies excite molecular vibrations in a variety of ways, making these methods suitable for specific products. Besides measurements of the fluid composition, technologies for particle detection are of additional importance. With scattered light techniques and evaluation according to the Mie theory, particles in the range of 0.2–1 µm can be detected, and fast growth processes can be observed. Local multispectral measurements can also be carried out with fiber optic-coupled systems through small probe heads of approximately 1 mm diameter.

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“…All samples can be assigned to their respective groups. Classical histology validates all measurements [13].…”

Section: Combination Techniques: Uv/vis/nir/fluorescencesupporting

confidence: 70%

Section: Particle Detectionmentioning

confidence: 99%

Section: Particle Detectionmentioning

confidence: 99%

Section: Combination Techniques: Uv/vis/nir/fluorescencementioning

confidence: 99%

“…Ultraviolet (UV), visible (VIS), NIR, and fluorescence measurements are possible. The needle is optimized for tissue penetration with a front and back cut[13].…”

mentioning

confidence: 99%

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Molecule Sensitive Optical Imaging and Monitoring Techniques—A Review of Applications in Micro-Process Engineering

2020

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Deconvolution of the fluorescence spectra measured through a needle probe to assess the functional state of the liver

et al. 2023

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ObjectivesCurrently, one of the most pressing issues for surgeons in the treatment of obstructive jaundice is the ability to assess the functional state of the liver and to detect and determine the degree of liver failure in a timely manner with simple and objective techniques. In this regard, the use of fluorescence spectroscopy method can be considered as one of the ways to improve the informativity of existing diagnostic algorithms in clinical practice and to introduce new diagnostic tools. Thus, the aim of the work was to study in vivo the functional state of liver parenchyma by the method of fluorescence spectroscopy implemented through a needle probe and assess the contribution of the main tissue fluorophores to reveal new diagnostic criteria.Materials and MethodsWe compared data from 20 patients diagnosed with obstructive jaundice and 11 patients without this syndrome. Measurements were performed using a fluorescence spectroscopy method at excitation wavelengths of 365 and 450 nm. Data were collected using a 1 mm fiber optic needle probe. The analysis was based on the comparison of the results of deconvolution with the combinations of Gaussian curves reflecting the contribution of the pure fluorophores in the liver tissues.ResultsThe results showed a statistically significant increase in the contribution of curves reflecting NAD(P)H fluorescence, bilirubin, and flavins in the group of patients with obstructive jaundice. This and the calculated redox ratio values indicated that the energy metabolism of the hepatocytes may have shifted to glycolysis due to hypoxia. An increase in vitamin A fluorescence was also observed. It may also serve as a marker of liver damage, indicating impaired vitamin A mobilization from the liver due to cholestasis.ConclusionsThe results obtained reflect changes associated with shifts in the content of the main fluorophores characterizing hepatocyte dysfunction caused by accumulation of bilirubin and bile acids and after disturbance of oxygen utilization. The contributions of NAD(P)H, flavins, and bilirubin as well as vitamin A can be used for further studies as promising diagnostic and prognostic markers for the course of liver failure. Further work will include collecting fluorescence spectroscopy data in patients with different clinical effects of obstructive jaundice on postoperative clinical outcome after biliary decompression.

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Optical percutaneous needle biopsy of the liver: a pilot animal and clinical study

Dremin

Potapova

Zherebtsov

et al. 2020

Sci Rep

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This paper presents the results of the experiments which were performed using the optical biopsy system specially developed for in vivo tissue classification during the percutaneous needle biopsy (PNB) of the liver. The proposed system includes an optical probe of small diameter acceptable for use in the PNB of the liver. The results of the feasibility studies and actual tests on laboratory mice with inoculated hepatocellular carcinoma and in clinical conditions on patients with liver tumors are presented and discussed. Monte Carlo simulations were carried out to assess the diagnostic volume and to trace the sensing depth. Fluorescence and diffuse reflectance spectroscopy measurements were used to monitor metabolic and morphological changes in tissues. The tissue oxygen saturation was evaluated using a recently developed approach to neural network fitting of diffuse reflectance spectra. The Support Vector Machine Classification was applied to identify intact liver and tumor tissues. Analysis of the obtained results shows the high sensitivity and specificity of the proposed multimodal method. This approach allows to obtain information before the tissue sample is taken, which makes it possible to significantly reduce the number of false-negative biopsies.

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A customized multispectral needle probe combined with a virtual photometric setup for in vivo detection of Lewis lung carcinoma in an animal model

Cited by 8 publications

References 52 publications

Molecule Sensitive Optical Imaging and Monitoring Techniques—A Review of Applications in Micro-Process Engineering

Molecule Sensitive Optical Imaging and Monitoring Techniques—A Review of Applications in Micro-Process Engineering

Deconvolution of the fluorescence spectra measured through a needle probe to assess the functional state of the liver

Optical percutaneous needle biopsy of the liver: a pilot animal and clinical study

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