A critical review on factors influencing fermentative hydrogen production

Abstract

Biohydrogen production by dark fermentation of different waste materials is a promising approach to produce bio-energy in terms of renewable energy exploration. This communication has reviewed various influencing factors of dark fermentation process with detailed account of determinants in biohydrogen production. It has also focused on different factors such as improved bacterial strain, reactor design, metabolic engineering and two stage processes to enhance the bioenergy productivity from substrate. The study also suggest that complete utilization of substrates for biological hydrogen production requires the concentrated research and development for efficient functioning of microorganism with integrated application for energy production and bioremediation. Various studies have been taken into account here, to show the comparative efficiency of different substrates and operating conditions with inhibitory factors and pretreatment option for biohydrogen production. The study reveals that an extensive research is needed to observe field efficiency of process using low cost substrates and integration of dark and photo fermentation process. Integrated approach of fermentation process will surely compete with conventional hydrogen process and replace it completely in future.

Sustainable Ammonia Production Processes

“…Dark fermentation is a fermentative conversion and versatile bioprocess for production of molecular biohydrogen from various biodegradable renewable feedstocks by means of a wide range of different types of bacteria (Kothari et al, 2017;Yaswanth, 2018). This process takes place in anoxic and anaerobic conditions (in the absence of oxygen) in which, during this process, a large spectrum of anaerobic fermentative bacteria such as clostridium, facultative anaerobes such as enterobacter, and aerobes such as bacillus are involved in the fermentation process (Ghimire et al, 2015).…”

Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives

“…In addition to the type of substrate, physical parameters (such as pH, agitation, temperature), the operating mode (the batch, continuous or semi-continuous process) and reactor design are other essential factors contributing to H 2 production, as reviewed in (Kothari et al 2017;Show et al 2008). It is necessary, for example, to maintain a low partial H 2 pressure to prevent inhibition by the product (Mizuno et al 2000).…”

Dynamics of bacterial communities and substrate conversion during olive-mill waste dark fermentation: Prediction of the metabolic routes for hydrogen production

“…Hydrogenases are the main enzyme that regulates the H2 metabolism and they are classified into three groups based on the number and identity of the metal in their active sites, such as nickel-iron [NiFe], iron-irons [FeFe] and iron [Fe] hydrogenases (Łukajtis et al, 2018). H2-producing proteins are linked to the efficiency of dark fermentative H2 production and also to the bacteria involved in the process (Kothari et al, 2017).…”