A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria

Glickmann, Eric; Dessaux, Yves

doi:10.1128/aem.61.2.793-796.1995

Cited by 903 publications

(436 citation statements)

References 13 publications

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“…We found that R. radiobacter (PABac-DSM) produces IAA in the presence of tryptophan. Despite the fact that Salkowski reagent also detects indole pyruvic acid and indole acetamide in addition to IAA (Glickmann and Dessaux, 1995), the method is fairly accurate because IAA is usually known as the main excreted microbial auxin. IAA is best known for its role in plant signal transduction (Quint and Gray, 2006).…”

Section: Discussionmentioning

confidence: 99%

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Sharma

Schmid

Rothballer

et al. 2008

Cellular Microbiology

150

137

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Because of their beneficial impact on plants, the highly diverse mycorrhizal fungi grouped in the order Sebacinales lay claim to high ecological and agricultural significance. Here, we describe for the first time associations of Sebacinoid members with bacteria. Using quantitative PCR, denaturating gradient gel electrophoresis and fluorescence in situ hybridization, we detected an intimate association between Piriformospora indica and Rhizobium radiobacter, an alpha-Proteobacterium. The stability of the association, vertical transmission of the bacteria during asexual fungal reproduction and fungal plant colonization was monitored using R. radiobacter-specific primers. Treatment of mycelium or fungal protoplasts with antibiotics highly efficient against the free bacteria failed to cure the fungus. Barley seedlings dip-inoculated with R. radiobacter showed growth promotion and systemic resistance to the powdery mildew fungus Blumeria graminis comparable to P. indica inoculation. By screening additional isolates of the Sebacina vermifera complex, three species-specific associations with bacteria from the genera Paenibacillus, Acinetobacter and Rhodococcus were found. These findings suggest that Sebacinales species regularly undergo complex interactions involving host plants and bacteria reminiscent of other ectomycorrhizal and endomycorrhizal associations.

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Section: Discussionmentioning

confidence: 99%

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Sharma

Schmid

Rothballer

et al. 2008

Cellular Microbiology

150

137

View full text Add to dashboard Cite

show abstract

“…Numerous plant-associated bacteria are able to produce auxin (3-indoleacetic acid [IAA]), a plant hormone of broad influence on plant growth and development, such as root architecture, vascular differentiation, and disease signaling, among many others. This assay, based on the Glickmann and Dessaux (1995) method, is used to measure the total amount of indolic compounds produce by a bacterial strain. However, it is not able to separate the amount of each "putative" indolic compound present in a sample (as IAA, indole-3-acetyl-trytophan [IA-Trp], indole-3-acetyl-aspartic acid [IA-Asp], and indole-3-acetyl-glutamic acid [IA-Glu] conjugates).…”

Section: Indolic Compound Production Assaymentioning

confidence: 99%

Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Ambrosini

Passaglia

2017

CP Plant Biology

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Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth–promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultural inoculants. Agricultural biofertilization and biocontrol of pathogens are eco‐friendly alternatives to chemical usage and have less energy, environmental, and economic costs. PGPB isolation and evaluation are essentials steps for determining bacteria that are able to improve plant development and productivity. In this unit, we present protocols to isolate bacteria from soil and plant roots (“putative” diazotrophic and endospore‐forming bacteria), as well to evaluate some of their beneficial characteristics for the promotion of plant growth (e.g., nitrogen fixation, production of indolic compounds and siderophores, phosphate solubilization, and 1‐aminocyclopropane‐1‐carboxylate deaminase activity). © 2017 by John Wiley & Sons, Inc.

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“…Previous experiments (S. Taghavi, unpublished results) have shown that Enterobacter sp. 638 is only able to produce low levels of IAA, <4 lg mL À1 after 48 h incubation with tryptophan under standard culture conditions (Schatz & Bovell, 1952;Glickmann & Dessaux, 1995). When grown in mineral media, Enterobacter sp.…”

Section: Introductionmentioning

confidence: 99%

Inoculation of hybrid poplar with the endophytic bacterium Enterobacter sp. 638 increases biomass but does not impact leaf level physiology

et al. 2011

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Endophytic bacteria have been shown to provide several advantages to their host, including enhanced growth. Inoculating biofuel species with endophytic bacteria is therefore an attractive option to increase the productivity of biofuel feedstocks. Here, we investigated the effect of inoculating hard wood cuttings of Populus deltoides Bartr. 9 Populus. nigra L. clone OP367 with Enterobacter sp. 638. After 17 weeks, plants inoculated with Enterobacter sp. 638 had 55% greater total biomass than un-inoculated control plants. Study of gas exchange and fluorescence in developing and mature leaves over a diurnal cycle and over a 5 week measurement campaign revealed no effects of inoculation on photosynthesis, stomatal conductance, photosynthetic water use efficiency or the maximum and operating efficiency of photosystem II. However, plants inoculated with Enterobacter sp. 638 had a canopy that was 39% larger than control plants indicating that the enhanced growth was fueled by increased leaf area, not by improved physiology. Leaf nitrogen content was determined at two stages over the 5 week measurement period. No effect of Enterobacter sp. 638 on leaf nitrogen content was found indicating that the larger plants were acquiring sufficient nitrogen. Enterobacter sp. 638 lacks the genes for N 2 fixation, therefore the increased availability of nitrogen likely resulted from enhanced nitrogen acquisition by the 84% larger root system. These data show that Enterobacter sp. 638 has the potential to dramatically increase productivity in poplar. If fully realized in the production environment, these results indicate that an increase in the environmental and economic viability of poplar as a biofuel feedstock is possible when inoculated with endophytic bacteria like Enterobacter sp. 638.

show abstract

A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria

Cited by 903 publications

References 13 publications

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Plant Growth–Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities

Inoculation of hybrid poplar with the endophytic bacterium Enterobacter sp. 638 increases biomass but does not impact leaf level physiology

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