A coupled geochemical and biogeochemical approach to characterize the bioreactivity of dissolved organic matter from a headwater stream

Sleighter, Rachel L.; Cory, Rose M.; Kaplan, Louis; Abdulla, Hussain; Hatcher, Patrick G.

doi:10.1002/2013jg002600

Cited by 77 publications

(76 citation statements)

References 84 publications

(157 reference statements)

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“…These NOM inputs are fresher and more labile; owing to their short residence times in soil they are less likely to undergo chemical or microbiological processes. Similar effects were demonstrated by Sleighter et al (2014), higher DOM biolabile concentrations were observed to the season of leaf fall due to increasing fresh DOM content in the leached. Biodegradation models of NOM 310 in aquatic environments showed that the degradation times of lignin residues to low-molecular (Dolgonosov and Gubernatorova, 2010).…”

Section: Quantification Of Nom Labile and Recalcitrant In Freshwater supporting

confidence: 79%

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Lability of natural organic matter in freshwater: a simple method for detection using hydrogen peroxide as an indicator

Constantino

Tadini

Lima

et al. 2018

Preprint

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Natural organic matter (NOM) is an important component for understanding the behavior of pollutants in the environment. A fraction of NOM is considered labile, fresh and less oxidized. 15In this work, a simple method was developed to distinguish between labile (LOM) and recalcitrant (ROM) organic matter in freshwater samples. Pyruvate, lignin and fulvic acid were chosen as model compounds of labile and recalcitrant NOM. The samples were submitted to kinetic monitoring experiments using hydrogen peroxide. Pyruvate was the best standard for the quantification of LOM (for concetrations up to 2.9 mg L -1 ). ROM was quantified by 20 measuring the difference between total organic carbon (TOC) and LOM concentrations. Curves obtained with 0.5 to 5.0 mg L -1 TOC (pyruvate) in freshwater or ultrapure water samples did not indicate the existence of a matrix effect. This simple method was applied to water samples that were collected monthly for one year; the resulting LOM concentrations ranged from 0.47 to 2.1 mg L -1 and the ROM concentrations ranged from 0.08 to 3.5 mg L -1 . Based on this results 25 we concluded that hydrogen peroxide kinetics can be used as a simple method to quantify LOM and ROM concentrations in freshwater samples.

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Section: Quantification Of Nom Labile and Recalcitrant In Freshwater supporting

confidence: 79%

“…Some authors have differentiated aquatic NOM by lability or recalcitrance (Leenheer;Croué, 2003;Filella, 2009;Lindell;Granéli;Tranvik, 1995;Miller et al, 1997;Sleighter et al, 2014;Uyguner-Demirel;Bekbolet, 2011). Labile organic matter (LOM) is defined as a fraction of NOM that is more biodegradable.…”

mentioning

confidence: 99%

Lability of natural organic matter in freshwater: a simple method for detection using hydrogen peroxide as an indicator

Constantino

Tadini

Lima

et al. 2018

Preprint

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“…However, a previous study found no difference in bacterial respiration between permafrost and modern DOM when degraded by aquatic bacteria in the dark, despite large differences in aromatic C content (Cory et al, 2013). Thus, aromatic C may control bacterial respiration in some waters, but the composition of the aromatics present may be as important as the abundance of aromatic C (Sleighter et al, 2014). For example, Mann et al (2014) proposed that increased phenolic aromatics within modern DOM inhibited aquatic bacterial respiration (Freeman et al, 2001), compared to ancient permafrost DOM containing less aromatic C. Alternatively, aromatic C content may not be a causal factor in bacterial respiration; that is, other facets of DOM composition may contribute to variability in bacterial activity, such as molecular weight or oxidation state (Meyer, 1994;Amon and Benner, 1996;Vallino et al, 1996;Sun et al, 1997).…”

Section: Introductionmentioning

confidence: 91%

Chemical composition of dissolved organic matter draining permafrost soils

Ward

Cory

2015

Geochimica et Cosmochimica Acta

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125

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Please cite this article as: Ward, C.P., Cory, R.M., Chemical composition of dissolved organic matter draining permafrost soils, Geochimica et Cosmochimica Acta (2015), doi: http://dx. AbstractNorthern circumpolar permafrost soils contain roughly twice the amount of carbon stored in the atmosphere today, but the majority of this soil organic carbon is perennially frozen.Climate warming in the Arctic is thawing permafrost soils and mobilizing previously frozen dissolved organic matter (DOM) from deeper soil layers to nearby surface waters. Previous studies have reported that ancient DOM draining deeper layers of permafrost soils was more susceptible to degradation by aquatic bacteria compared to modern DOM draining the shallow active layer of permafrost soils, and have suggested that DOM chemical composition may be an important control for the lability of DOM to bacterial degradation. However, the compositional features that distinguish DOM drained from different depths in permafrost soils are poorly characterized. Thus, the objective of this study was to characterize the chemical composition of DOM drained from different depths in permafrost soils, and relate these compositional differences to its susceptibility to biological degradation. DOM was leached from the shallow organic mat and the deeper permafrost layer of soils within the Imnavait Creek watershed on the North Slope of Alaska. DOM draining both soil layers was characterized in triplicate by coupling ultra-high resolution mass spectrometry, 13 C solid-state NMR, and optical spectroscopy methods with multi-variate statistical analyses. Reproducibility of replicate mass spectra was high, and compositional differences resulting from interfering species or isolation effects were significantly smaller than differences between DOM drained from each soil layer. All analyses indicated that DOM leached from the shallower organic mat contained higher molecular weight, more oxidized, and more unsaturated aromatic species compared to DOM leached from the deeper permafrost layer. Bacterial production rates and bacterial efficiencies were significantly higher for permafrost compared to organic mat DOM; however, respiration rates were similar 3 between DOM sources. Increased release of permafrost DOM from arctic soils to surface waters will change the chemical composition of DOM and its lability to bacteria, but this study suggests that these shifts in DOM composition and lability may not increase the carbon dioxide produced by bacterial respiration of permafrost DOM exported to arctic surface waters compared to DOM currently draining the shallow active layer.4

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“…This exemplifies that the exact chemical composition might be a more universal predictor of decay patterns than the degree of allochthony. With the increasing number of studies using the ultrahigh resolution mass spectrometry to understand the molecular drivers of DOM reactivity in a variety of systems (Gonsior et al, 2013;Sleighter et al, 2014;D'Andrilli et al, 2015;Kellerman et al, 2015;Hawkes et al, 2016b;Riedel et al, 2016;Kamjunke et al, 2017) a better understanding of the intrinsic controls of aquatic DOM reactivity becomes increasingly possible to achieve.…”

Section: General Trends In Reactivity Among Major Compound Groupsmentioning

confidence: 99%

Molecular Determinants of Dissolved Organic Matter Reactivity in Lake Water

et al. 2017

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Lakes in the boreal region have been recognized as the biogeochemical hotspots, yet many questions regarding the regulators of organic matter processing in these systems remain open. Molecular composition can be an important determinant of dissolved organic matter (DOM) fate in freshwater systems, but many aspects of this relationship remain unclear due to the complexity of DOM and its interactions in the natural environment. Here, we combine ultrahigh resolution mass spectrometry (FT-ICR-MS) with kinetic modeling of decay of >1,300 individual DOM molecular formulae identified by mass spectrometry, to evaluate the role of specific molecular characteristics in decomposition of lake water DOM. Our data is derived from a 4 months microbial decomposition experiment, carried out on water from three Swedish lakes, with the set-up including natural lake water, as well as the lake water pretreated with UV light. The relative decay rate of every molecular formula was estimated by fitting a single exponential model to the change in FT-ICR-MS signal intensities over decomposition time. We found a continuous range of exponential decay coefficients (k exp ) within different groups of compounds and show that for highly unsaturated and phenolic compounds the distribution of k exp was shifted toward the lowest values. Contrary to this general trend, plant-derived polyphenols and polycondensed aromatics were on average more reactive than compounds with an intermediate aromaticity. The decay rate of aromatic compounds increased with increasing nominal oxidation state of carbon, and molecular mass in some cases showed an inverse relationship with k exp in the UV-manipulated treatment. Further, we observe an increase in formulae-specific k exp as a result of the UV pretreatment. General trends in reactivity identified among major compound groups emphasize the importance of the intrinsic controllers of lake water DOM decay. However, we additionally indicate that each compound group contained a wide spectrum of reactivities, suggesting that high resolution is needed to further ascertain the complex reasons behind DOM reactivity in lake water.

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A coupled geochemical and biogeochemical approach to characterize the bioreactivity of dissolved organic matter from a headwater stream

Cited by 77 publications

References 84 publications

Lability of natural organic matter in freshwater: a simple method for detection using hydrogen peroxide as an indicator

Lability of natural organic matter in freshwater: a simple method for detection using hydrogen peroxide as an indicator

Chemical composition of dissolved organic matter draining permafrost soils

Molecular Determinants of Dissolved Organic Matter Reactivity in Lake Water

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