A coumarin/cyanoacetamide-derived lysosome-targeting probe for detecting bisulfite and viscosity changes in living cells and zebrafish

A fluorescence “turn-on” probe for the detection of HSO3− based on a pyrene-functionalized mesoporous silica material

“…Thus, all of the LUMO, HOMO and the HOMO–LUMO energy gap of PYA-SBA-15-HSO 3 − were higher than those of PYA-SBA-15, which theoretically supported the enhancement in fluorescence intensity and confirmed that the detection mechanism of PYA-SBA-15 toward HSO 3 − was due to quick reaction of the carbon–nitrogen double bond in PYA-SBA-15 with HSO 3 − in the Michael addition reaction. 20,21,41–44…”

“…4,8–11 Thus, great efforts have been made to develop a number of organic-based fluorescent probes for the detection of HSO 3 − . 2,12 − 16 However, these organic-based probes, such as iridium complex, 17,18 rhodamine 19 and coumarin, 20 and the probes based on the cooperative function of serum albumins (such as BSA and HSA) and HSO 3 − 21 − 24 are greatly limited by the complexity of their synthesis, poor selectivity for HSO 3 − , poor water solubility and the need for organic solvents. 19,25 In order to combine the advantages of inorganic and organic functional groups, organic–inorganic hybrid-based nanomaterials, such as quantum dots and metal–organic frameworks, have been designed as fluorescent probes for the detection of HSO 3 − .…”

“…4,[8][9][10][11] Thus, great efforts have been made to develop a number of organic-based fluorescent probes for the detection of HSO 3 À . 2,12À16 However, these organic-based probes, such as iridium complex, 17,18 rhodamine 19 and coumarin, 20 and the probes based on the cooperative function of serum albumins (such as BSA and HSA) and HSO 3 À21À24 are greatly limited by the complexity of their synthesis, poor selectivity for HSO 3…”

Investigating Lysosomal Reactive Sulphur Species with Small Molecular Fluorescent Probes

“…In 2022, Wu's group proposed fluorescent probe 41 for detecting HSO 3 − fluctuations in live cells and zebrafish (Figure 28). [108] Coumarin served as the fluorophore, C=C as the recognition group and morpholine as the lysosome‐targeting group. The probe itself showed weak fluorescence at 575 nm.…”