A correlative study of the levels of salivary Streptococcus mutans, lactobacilli and Actinomyces with dental caries experience in subjects with mixed and permanent dentition

Chokshi, Achala; Mahesh, Pushpalatha; Sharada, P; Chokshi, Krunal; Anupriya, S; Ashwini, B K

doi:10.4103/0973-029x.180916

Cited by 27 publications

(16 citation statements)

References 20 publications

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“…However, S. mutans is still believed to be the principle cariogenic bacterium [5]. S. mutans possesses primary acidogenic and aciduric abilities causing unbalanced pH fluctuations even under salivary buffering, and it can produce large amounts of exopolysaccharides (EPS), which contribute greatly to biofilm formation [6,7]. Thus, targeting S. mutans could prove to be an effective means of preventing or arresting dental caries.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats

Wang

Zeng

et al. 2018

Journal of Oral Microbiology

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Background:Targeting Streptococcus mutans, the principle cariogenic bacterium, could prove to be an effective means of preventing dental caries. A de novo designed antimicrobial peptide, GH12, has shown bactericidal effects on S. mutans and inhibitory effects on virulence and regulatory systems of S. mutans. However, the effects of GH12 on caries remain to be elucidated.Objectives: The objectives of this study were to evaluate the direct effects of GH12 on caries and numbers of S. mutansin vivo.Design: The Keyes score was evaluated in a rodent model and in vivo S. mutans was quantified by qPCR. To further interpret the in vivo anticaries efficacy, an in vitro biofilm model using short-term treatments with GH12 mimicked treatments in vivo was adopted. Results: In vivo data showed that GH12 at 8 mg/L reduced the incidence and severity of caries. Furthermore, GH12-treated animals had less S. mutans infection. In vitro data revealed that GH12 reduced the number of S. mutans within the biofilm, as well as reducing lactic acid and water-insoluble EPS synthesis of S. mutans biofilms. Afterwards, all rats showed no significant difference in weight gain, and no sign of harm to the mucosa, indicating that GH12 possessed good biocompatibility in vivo.Conclusion: Due to the combined inhibitory effects of GH12 on the biomass and cariogenic properties of S. mutans biofilms, the population of S. mutans in vivo was reduced, residual S. mutans biofilms were less acidic and compact, and thereby the onset and development of caries were arrested. Such findings collectively certified the clinical prospects for GH12. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats

Wang

Zeng

et al. 2018

Journal of Oral Microbiology

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“…Dental caries is a major infectious disease affecting the majority of the world's population [1]. It is considered one of the most prevalent diseases, particularly in socially underdeveloped communities [2].…”

Section: Introductionmentioning

confidence: 99%

Correlation between dental caries experience and the level of Streptococcus mutans and lactobacilli in saliva and carious teeth in a Yemeni adult population

Sounah

Madfa

2020

BMC Res Notes

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Objective: This study aimed to determine the relative amounts of Streptococcus mutans (SM) and lactobacilli (LBs) and their relationship with dental caries among a Yemeni adult population. Results: A positive correlation appeared between SM and LB counts from saliva and caries tissue samples and the decayed, missing and filled teeth (DMFT) score (p < 0.05). There was a significant correlation between SM and LBs in caries tissue (p < 0.05). However, there was no significant difference between SM and LBs isolated from saliva samples (p > 0.05). The number of SM and LBs in subjects with active caries was significantly higher than that in those without active caries (p < 0.05). There was no significant difference between the daily habits and SM and LB or DMFT scores (p > 0.05), except for a significant difference between brushing frequency and DMFT score (p < 0.05).

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“…A health-associated biofilm includes genera such as Neisseria, Streptococcus, Actinomyces, Veillonella, and Granulicatella [9]. There is evidence that a number of oral bacteria, like Streptococcus mutans, Actinomyces spp., and Lactobacillus spp., which are the components of the normal microbial flora, are positively related to decayed, missing, and filled teeth (DMFT) index [10]. Previous studies have implicated specific bacteria in the development and progression of periodontal diseases.…”

Section: Introductionmentioning

confidence: 99%

Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria

et al. 2019

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Dental caries and periodontal diseases are associated with a shift from symbiotic microbiota to dysbiosis. The aim of our study was to develop a rapid, sensitive, and economical method for the identification and quantification of selected cariogenic and periodontal oral bacteria. Original protocols were designed for three real-time multiplex PCR assays to detect and quantify the ratio of 10 bacterial species associated with dental caries (“cariogenic” complex) or periodontal diseases (red complex, orange complex, and Aggregatibacter actinomycetemcomitans). A total number of 60 samples from 30 children aged 2–6 years with severe early childhood caries and gingivitis were tested. In multiplex assays, the quantification of total bacterial (TB) content for cariogenic bacteria and red complex to eliminate differences in quantities caused by specimen collection was included. The mean counts for the TB load and that of ten evaluated specimens corresponded to previously published results. We found a significant difference between the microbial compositions obtained from the area of control and the affected teeth (p < 0.05). Based on this comprehensive microbiological examination, the risk of dental caries or periodontal inflammation may be determined. The test could also be used as a tool for behavioral intervention and thus prevention of the above-mentioned diseases.

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A correlative study of the levels of salivary Streptococcus mutans, lactobacilli and Actinomyces with dental caries experience in subjects with mixed and permanent dentition

Cited by 27 publications

References 20 publications

Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats

Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats

Correlation between dental caries experience and the level of Streptococcus mutans and lactobacilli in saliva and carious teeth in a Yemeni adult population

Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria

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