A correlative spatiotemporal microscale study of calcium phosphate formation and transformation within an alginate hydrogel matrix

Abstract

The precipitation and transformations of calcium phosphates (CaP) is a complex process, where both formation kinetics and the stability of different mineral phases control the outcome. This situation is even more complex if CaP is precipitated in a hydrogel matrix, where one can expect the organic matrix to modulate crystallization by introducing supersaturation gradients or changing the nucleation and growth kinetics of crystals. In this study we apply a range of characterization techniques to study the mineral formation and transformations of CaP within an alginate matrix with spatiotemporal resolution. It demonstrates how a detailed investigation of the mineral precipitation and transformations can aid in the future rational design of hydrogel-based materials for bone tissue engineering and studies of biomineralization processes.

Micro-Raman Spectroscopy Reveals the Presence of Octacalcium Phosphate and Whitlockite in Association with Bacteria-Free Zones Within the Mineralized Dental Biofilm

“…Bact– consist predominantly of OCP and WL inclusions. OCP is identified by a split ν 1 PO 4 3− band and the presence of acid phosphate (HPO 4 2− ) groups (Bannerman et al, 2016; Bjørnøy et al, 2016; Shi et al, 2016). WL exhibits a shift of the ν 1 PO 4 3− band to higher wavenumbers, from ~960 cm −1 (for CHAp) to 968–970 cm −1 , and a shift of the ν 2 PO 4 3− band to lower wavenumbers, from ~430 cm −1 (for CHAp) to 407 cm −1 (de Aza et al, 1997; Wopenka & Pasteris, 2005).…”

Preparation of CaO@TiO2–SiO2 Biomaterial with a Sol–Gel Method for Bone Implantation

“… 17 The layered spherical materials with a hierarchical structure, which are designed to fill the volumes of the complex shapes during the restoration of bone tissue, have been used as biomedical materials. 18 , 19 To improve the functional properties of the implant material, the compositions of calcium phosphate, silicon, and titanium are crucial. 20 − 24 It has been reported that the presence of silicon on the surface of the phosphate–calcium material accelerates the bonding of the implant with the bone (osseointegration).…”

The effect of sodium and magnesium ions on the properties of calcium–phosphate biomaterials

“…The process of bone tissue remodeling occurs about twice faster on silicon-containing hydroxyapatite (SiHA) than on hydroxyapatite in the absence of silicon (Petrovskaya et al 2016; Bjornoy et al 2016). …”

“…Silicate groups in the calcium–phosphate system significantly increase the rate of osteogenesis in vivo after implantation. The process of bone tissue remodeling occurs about twice faster on silicon-containing hydroxyapatite (SiHA) than on hydroxyapatite in the absence of silicon (Petrovskaya et al 2016; Bjornoy et al 2016).…”