A Correction to the Autoanalyzer Data for Quantitative Agglutination Measurements

Abstract

The AutoAnalyzer was used for quantitative measurements of antibody agglutinating activity by a Polybrene agglutination technique. Since the continuous flow system introduces an experimental error to the determination of true agglutination percentage, it is necessary to apply a correction factor. The experimental method of correction is described.

Activity of IgG and IgM ABO Antibodies against Some Weak A (A3, Ax, Aend) and Weak B (B3, Bx) Red Cells

“…The reactions were left 2 h at 25 OC with continuous shaking and after centrifugation, the supernatants were removed and their anti-A activity tested against A, cells in a brornelin-methyl cellulose autoanalyzer system. For each dilution a percentage of agglutination was calculated using the method age of agglutination was calculated using the method of Doinef et al [2]: the results were plotted in the log-probit assay 191 and the HD,, of each agglutinin after fixation on the various A RBC was determined; the HD,, of unabsorbed IgM and IgG anti-A were tested in parallel. The amounts of anti-A activity fixed on A,, A, and Ax RBC were determined by subtracting the corresponding HD6@ from the HD,, of the unabsorbed fractions.…”

Groups of α‐d‐Galactosyltransferase Activity in Sera of Individuals with Normal B Phenotype

“…For each red cell sample, the log-probit hemagglutination assay was carried out at 2OoC using the autoanalyser. The method, derived from the polybrene technique, has been described by DOINEL et al [5]. In preliminary measurements, four different anti-B antisera were used, i.e.…”