“…Nevertheless, the non-enzymatic proteins also represent a suitable repertoire of such potential scaffolds, which could be used for development as sugar-binding proteins to be used in reactors for simultaneous separation of sugars that would be used in subsequent conversion steps. We have developed a RuBP production scheme from 3PGA [16,17] and also a de novo RuBP production scheme from D-glucose [21] for continuous CO 2 fixation and for start-up of the fixation respectively employing series of reactors. Both systems for production of RuBP will benefit from specific sugar binders but besides their use in environmental biotechnology, they will find application in diagnostics, separation technologies and also as research reagents.…”
Section: Resultsmentioning
confidence: 99%
“…However, inasmuch as the recycling of acceptor RuBP is central to continuous CO 2 fixation, we have invented a novel scheme (Figure 1), which proceeds with no loss of CO 2 (unlike cellular biochemical systems) in 11 steps in a series of bioreactors [20]. This scheme is very different from generation of RuBP from D-glucose for start-up process [21] and employing 11 steps in different reactors requiring large volume and weight. The linear combination of reactors with large volume and weight are unsuitable for use with mobile CO 2 emitters leaving only the stationary source of emission to be controlled using this technology [17].…”
Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology.
“…Nevertheless, the non-enzymatic proteins also represent a suitable repertoire of such potential scaffolds, which could be used for development as sugar-binding proteins to be used in reactors for simultaneous separation of sugars that would be used in subsequent conversion steps. We have developed a RuBP production scheme from 3PGA [16,17] and also a de novo RuBP production scheme from D-glucose [21] for continuous CO 2 fixation and for start-up of the fixation respectively employing series of reactors. Both systems for production of RuBP will benefit from specific sugar binders but besides their use in environmental biotechnology, they will find application in diagnostics, separation technologies and also as research reagents.…”
Section: Resultsmentioning
confidence: 99%
“…However, inasmuch as the recycling of acceptor RuBP is central to continuous CO 2 fixation, we have invented a novel scheme (Figure 1), which proceeds with no loss of CO 2 (unlike cellular biochemical systems) in 11 steps in a series of bioreactors [20]. This scheme is very different from generation of RuBP from D-glucose for start-up process [21] and employing 11 steps in different reactors requiring large volume and weight. The linear combination of reactors with large volume and weight are unsuitable for use with mobile CO 2 emitters leaving only the stationary source of emission to be controlled using this technology [17].…”
Sugar binding proteins and binders of intermediate sugar metabolites derived from microbes are increasingly being used as reagents in new and expanding areas of biotechnology.
“…Bhattacharya et al . developed and patented a novel process for CO 2 capture. They utilized fundamental metabolic pathways of green plant for CO 2 fixation.…”
Section: Ca and Bioreactormentioning
confidence: 99%
“…Fradette [60] patented a spray tower reactor for CO 2 removal by using free or immobilized CA on fine particles [60]. Bhattacharya et al [61] developed and patented a novel process for CO 2 capture. They utilized fundamental metabolic pathways of green plant for CO 2 fixation.…”
Anthropogenic activities have substantially increased the level of greenhouse gases (GHGs) in the atmosphere and are contributing significantly to the global warming. Carbon dioxide (CO2 ) is one of the major GHGs which plays a key role in the climate change. Various approaches and methodologies are under investigation to address CO2 capture and sequestration worldwide. Carbonic anhydrase (CA) mediated CO2 sequestration is one of the promising options. Therefore, the present review elaborates recent developments in CA, its immobilization and bioreactor methodologies towards CO2 sequestration using the CA enzyme. The promises and challenges associated with the efficient utilization of CA for CO2 sequestration and scale up from flask to lab-scale bioreactor are critically discussed. Finally, the current review also recommends the possible future needs and directions to utilize CA for CO2 sequestration.
“…For starting up the process, however, a different scheme was used to generate RuBP from D-glucose rather than from 3-PGA [14]. The linear combination of reactors in the 11 step RuBP regeneration process requires large volume and weight and are unsuitable for use in mobile CO 2 emitters leaving only the stationary source of emission to be controlled using this technology [8,9].…”
Background: Carbon dioxide fixation bioprocess in reactors necessitates recycling of D-ribulose1,5-bisphosphate (RuBP) for continuous operation. A radically new close loop of RuBP regenerating reactor design has been proposed that will harbor enzyme-complexes instead of purified enzymes. These reactors will need binders enabling selective capture and release of sugar and intermediate metabolites enabling specific conversions during regeneration. In the current manuscript we describe properties of proteins that will act as potential binders in RuBP regeneration reactors.
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