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De, Debojyoti; Dutta, Debajyoti; Kundu, Moloy; Mahato, Sourav; Schiavone, Marc; Chaudhuri, Surabhi; Giri, Ashok P.; Gupta, Vidya S.; Bhattacharya, Sanjoy K.

doi:10.1186/1475-2859-4-5

Cited by 1 publication

(1 citation statement)

References 22 publications

(26 reference statements)

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“…A single major contaminating protein in both human and mouse preparations was identified by mass spectrometry as alpha-enolase, a 48 kDa glycolytic enzyme, with a Mascot score of 159 (6 peptides, 19% sequence coverage). Secreted alpha enolase binds CM cellulose 20 21 22 23 24 .…”

Section: Resultsmentioning

confidence: 99%

Eukaryotic expression, purification and structure/function analysis of native, recombinant CRISP3 from human and mouse

Volpert

Mangum

Jamsai

et al. 2014

Sci Rep

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While the Cysteine-Rich Secretory Proteins (CRISPs) have been broadly proposed as regulators of reproduction and immunity, physiological roles have yet to be established for individual members of this family. Past efforts to investigate their functions have been limited by the difficulty of purifying correctly folded CRISPs from bacterial expression systems, which yield low quantities of correctly folded protein containing the eight disulfide bonds that define the CRISP family. Here we report the expression and purification of native, glycosylated CRISP3 from human and mouse, expressed in HEK 293 cells and isolated using ion exchange and size exclusion chromatography. Functional authenticity was verified by substrate-affinity, native glycosylation characteristics and quaternary structure (monomer in solution). Validated protein was used in comparative structure/function studies to characterise sites and patterns of N-glycosylation in CRISP3, revealing interesting inter-species differences.

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