A continuous control mode with improved imaging rate for scanning ion conductance microscope (SICM)

Zhuang, Jian; Jiao, Yangbohan; Li, Zeqing; Lang, Jinxin; Li, Fēi

doi:10.1016/j.ultramic.2018.04.009

Cited by 12 publications

(7 citation statements)

References 37 publications

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“…The MSE can reflect the stability of the scanning. 33 A higher MSE means higher noise or greater fluctuation in the sample topography and lower reliability and higher collision risk during scanning. Selection of the fastest speed is also dependent on the MSE.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability

et al. 2021

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Scanning ion conductance microscopy (SICM) is a type of in situ measurement technology for noncontact detection of samples in electrolytes with nanoscale resolution and has been used increasingly in biomedical and electrochemical fields in recent years. However, there is an inherent contradiction in the technique that makes SICM's sensitivity and accuracy difficult to balance. Higher sensitivity allows for faster probe speeds and higher scanning reliability but leads to lower accuracy, and vice versa. To resolve this problem, an adaptive sensitivity scanning method is proposed here that is designed to increase SICM's imaging efficiency without reducing its scanning reliability and accuracy. In the proposed scanning method, the sensitivity is automatically switched via the bias voltage based on the probe−sample distance. When the probe is located far away from the sample, the probe then predetects the sample position rapidly with high sensitivity. When the sample has been sensed in the high-sensitivity phase, the probe then detects the sample with low sensitivity. The basic theory and the feasibility of the alterable sensitivity detection strategy is also studied using the finite element method (FEM) and by performing experiments in this work. Finally, through testing of the standard silicon and polydimethylsiloxane (PDMS) samples, the proposed method is shown to increase SICM imaging efficiency significantly by up to 5 times relative to the conventional hopping mode without sacrificing the scanning accuracy and reliability.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…K denotes the number of scans of a specified sample area. The MSE can reflect the stability of the scanning . A higher MSE means higher noise or greater fluctuation in the sample topography and lower reliability and higher collision risk during scanning.…”

Section: Resultsmentioning

confidence: 99%

Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability

et al. 2021

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“…Together, BMI-1 cells and SICM methods offer a number of advantages when studying the ASL physiology and/or the effects of medicines targeting the ASL. Further, by using twin barrel SICM probes, for example, for scanning and pH measurement, , and implementing software automation and faster scanning, there are obvious ways to increase the speed and versatility of these methods even further.…”

Section: Discussionmentioning

confidence: 99%

A Nanosensor Toolbox for Rapid, Label-Free Measurement of Airway Surface Liquid and Epithelial Cell Function

Ivanova

Benton

Munye

et al. 2019

ACS Appl. Mater. Interfaces

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Ciliated lung epithelial cells and the airway surface liquid (ASL) comprise one of the body's most important protective systems. This system is finely tuned, and perturbations to ASL rheology, ASL depth, ASL pH, the transepithelial potential, and the cilia beat frequency are all associated with disease pathology. Further, these apparently distinct properties interact with each other in a complex manner. For example, changes in ASL rheology can result from altered mucin secretion, changes in ASL pH, or changes in ASL depth. Thus, one of the great challenges in trying to understand airway pathology is that the properties of the ASL/epithelial cell system need to be assessed nearsimultaneously and without perturbing the sample. Here, we show that nanosensor probes mounted on a scanning ion conductance microscope make this possible for the first time, without any need for labeling. We also demonstrate that ASL from senescenceretarded human bronchial epithelial cells retains its native properties. Our results demonstrate that by using a nanosensor approach, it is possible to pursue faster, more accurate, more coherent, and more informative studies of ASL and airway epithelia in health and disease.

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“…Essentially, these modes constantly perform approach curves, although over controlled, short distances. Approach retract scanning (ARS) mode − further divides the approach process into two components: a coarse approach region and a fine approach region dictated by changes in the ion current magnitude. A faster approach speed is used during the coarse approach until an intermediate set point (e.g., 99.5% of the steady-state current) is achieved.…”

Section: Development Of Sicmmentioning

confidence: 99%

Scanning Ion Conductance Microscopy

et al. 2020

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Scanning ion conductance microscopy (SICM) has emerged as a versatile tool for studies of interfaces in biology and materials science with notable utility in biophysical and electrochemical measurements. The heart of the SICM is a nanometer-scale electrolyte filled glass pipette that serves as a scanning probe. In the initial conception, manipulations of ion currents through the tip of the pipette and appropriate positioning hardware provided a route to recording micro-and nanoscopic mapping of the topography of surfaces. Subsequent advances in instrumentation, probe design, and methods significantly increased opportunities for SICM beyond recording topography. Hybridization of SICM with coincident characterization techniques such as optical microscopy and faradaic electrodes have brought SICM to the forefront as a tool for nanoscale chemical measurement for a wide range of applications. Modern approaches to SICM realize an important tool in analytical, bioanalytical, biophysical, and materials measurements, where significant opportunities remain for further exploration. In this review, we chronicle the development of SICM from the perspective of both the development of instrumentation and methods and the breadth of measurements performed. CONTENTS

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A continuous control mode with improved imaging rate for scanning ion conductance microscope (SICM)

Cited by 12 publications

References 37 publications

Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability

Research on the Adaptive Sensitivity Scanning Method for Ion Conductance Microscopy with High Efficiency and Reliability

A Nanosensor Toolbox for Rapid, Label-Free Measurement of Airway Surface Liquid and Epithelial Cell Function

Scanning Ion Conductance Microscopy

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