A constructed wetland for treatment of an impacted waterway and the influence of native waterfowl on its perceived effectiveness

McMinn, Brian R.; Klemm, Sara; Korajkic, Asja; Wyatt, Kimberly M.; Herrmann, Michael P.; Haugland, Richard A.; Lu, Jingrang; Villegas, Eric N.; Frye, Craig

doi:10.1016/j.ecoleng.2018.11.026

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…The wetland sampling has been described in our previous study [ 11 ]. Briefly, samples were collected from June through September 2017 from five sites in the Banklick Creek Treatment Wetland located in Fort Wright, Kentucky (39°01′14.2″ N 84°31′42.1″ W) ( Figure S1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Nucleic acids were then extracted from the supernatant using the AllPrep DNA/RNA Mini Kit (Qiagen, Germantown, MD, USA), following the manufacturer’s instructions. A Campylobacter genus-specific PCR assay was used to amplify 28 of the samples which were positive via the previous qPCR assay for Campylobacter genera [ 11 , 20 , 28 ] ( Table S1 ). The amplicons were cloned into pCR4.1 TOPO (Invitrogen, Carlsbad, CA, USA) to aid in further identification.…”

Section: Methodsmentioning

confidence: 99%

“…Previous research using qPCR analyses of the treated water throughout the system showed that Campylobacter and avian marker signals (targeting Helobacter sp.) were detected at high frequencies where bird occupancy was commonly observed [ 11 ]. Moreover, various waterfowl species, such as Canada goose, were frequently found throughout the CTW.…”

Section: Introductionmentioning

confidence: 99%

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Genomic Characterization and Wetland Occurrence of a Novel Campylobacter Isolate from Canada Geese

et al. 2023

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Populations of resident, non-migratory Canada geese are rapidly increasing. Canada geese are known to transmit viral and bacterial diseases, posing a possible threat to human health. The most prevalent pathogens vectored by geese are Campylobacter species, yet the current understanding of the identity and virulence of these pathogens is limited. In our previous study, we observed a high prevalence of Campylobacter spp. in the Banklick Creek wetland—a constructed treatment wetland (CTW) located in northern KY (USA) used to understand sources of fecal contamination originating from humans and waterfowl frequenting the area. To identify the types of Campylobacter spp. found contaminating the CTW, we performed genetic analyses of Campylobacter 16s ribosomal RNA amplified from CTW water samples and collected fecal material from birds frequenting those areas. Our results showed a high occurrence of a Campylobacter canadensis-like clade from the sampling sites. Whole-genome sequence analyses of an isolate from Canada goose fecal material, called MG1, were used to confirm the identity of the CTW isolates. Further, we examined the phylogenomic position, virulence gene content, and antimicrobial resistance gene profile of MG1. Lastly, we developed an MG1-specific real-time PCR assay and confirmed the presence of MG1 in Canada goose fecal samples surrounding the CTW. Our findings reveal that the Canada goose-vectored Campylobacter sp. MG1 is a novel isolate compared to C. canadensis that possesses possible zoonotic potential, which may be of human health concern.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic Characterization and Wetland Occurrence of a Novel Campylobacter Isolate from Canada Geese

et al. 2023

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“…There were no changes in concentration of fecal indicator during the treatment period, and the human marker HF183 concentration was lowered significantly at later stages than that of beginning stage of treatment. Furthermore, at the later stage treatment Campylobacter signals were significantly higher and suggested that relying on general fecal indicator for determination of CWs efficacy is not an ideal condition always (McMinn et al, 2019).…”

Section: Wetlands For Pathogens and Virus Removalmentioning

confidence: 99%

Wetlands for environmental protection

Martinez‐Guerra

Ghimire

Nandimandalam

et al. 2020

Water Environment Research

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This article presents an update on the research and practical demonstration of wetland-based treatment technologies for protecting water resources and environment covering papers published in 2019. Wetland applications in wastewater treatment, stormwater management, and removal of nutrients, metals, and emerging pollutants including pathogens are highlighted. A summary of studies focusing on the effects of vegetation, wetland design and operation strategies, and process configurations and modeling, for efficient treatment of various municipal and industrial wastewaters, is included. In addition, hybrid and innovative processes with wetlands as a platform treatment technology are presented.

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“…Fecal pollution management represents a major challenge for water quality authorities worldwide. Fecal waste can enter waterways from a variety of sources such as leaky sewer lines, faulty septic systems, stormwater run-off, improper agricultural waste management practices, and local wildlife [1–5]. When fecal pollution is present in surface waters, it can represent a public health risk due to the presence of disease causing pathogens (see [6] for review), as well as lead to severe economic burdens [7].…”

Section: Introductionmentioning

confidence: 99%

Large-scale implementation of standardized quantitative real-time PCR fecal source identification procedures in the Tillamook Bay Watershed

Sivaganesan

Kelty

et al. 2019

PLoS ONE

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Fecal pollution management remains one of the biggest challenges for water quality authorities worldwide. Advanced fecal pollution source identification technologies are now available that can provide quantitative information from many animal groups. As public interest in these methodologies grows, it is vital to use standardized procedures with clearly defined data acceptance metrics and conduct field studies demonstrating the use of these techniques to help resolve real-world water quality challenges. Here we apply recently standardized human-associated qPCR methods with custom data acceptance metrics (HF183/BacR287 and HumM2), along with established procedures for ruminant (Rum2Bac), cattle (CowM2 and CowM3), canine (DG3 and DG37), and avian (GFD) fecal pollution sources to (i) demonstrate the feasibility of implementing standardized qPCR procedures in a large-scale field study, and (ii) characterize trends in fecal pollution sources in the research area. A total of 602 water samples were collected over a one-year period at 29 sites along the Trask, Kilchis, and Tillamook rivers and tributaries in the Tillamook Bay Watershed (OR, USA). Host-associated qPCR results were combined with high-resolution geographic information system (GIS) land use and general indicator bacteria ( E . coli ) measurements to elucidate water quality fecal pollution trends. Results demonstrate the feasibility of implementing standardized fecal source identification qPCR methods with established data acceptance metrics in a large-scale field study leading to new investigative leads suggesting that elevated E . coli levels may be linked to specific pollution sources and land use activities in the Tillamook Bay Watershed.

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A constructed wetland for treatment of an impacted waterway and the influence of native waterfowl on its perceived effectiveness

Cited by 13 publications

References 40 publications

Genomic Characterization and Wetland Occurrence of a Novel Campylobacter Isolate from Canada Geese

Genomic Characterization and Wetland Occurrence of a Novel Campylobacter Isolate from Canada Geese

Wetlands for environmental protection

Large-scale implementation of standardized quantitative real-time PCR fecal source identification procedures in the Tillamook Bay Watershed

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