A computer program for determining the size of DNA restriction fragments

Duggleby, Ronald G.; Kinns, H.R.; Rood, Julian I.

doi:10.1016/0003-2697(81)90110-x

Cited by 85 publications

(35 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…haze filter in tandem. Photographic negatives were scanned with an LKB densitometer in order to determine the position of each EcoRI fragment and the number of EcoRl fragments in each band; the computer program of Duggleby et al (1981) was used to determine the sizes, in kilobase pairs (kb), of DNA restriction fragments of the five plaque-purified isolates. The relative number of fragments in each band was estimated by cutting out and weighing each peak after scanning densitometry of the Tri-X negatives.…”

Section: Cells and Virusmentioning

confidence: 99%

African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

Wesley

Pan

1982

Journal of General Virology

View full text Add to dashboard Cite

Section: Cells and Virusmentioning

confidence: 99%

African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

Wesley

Pan

1982

Journal of General Virology

View full text Add to dashboard Cite

“…Fragment sizes were calculated from the regression line, based on a set of standard-sized fragments (lambda DNA-Hind -III/phiX-174 RF DNA-Hinc digest), using a modification of the computer program described by Duggleby et al (24). Adjustment was made for the 5% variability in fragment sizes observed in replicate samples on different blots.…”

Section: Methodsmentioning

confidence: 99%

Association of a 9.2‐kilobase pvu ii class i major histocompatibility complex restriction fragment length polymorphism with ankylosing spondylitis

McDaniel

Acton

Barger

et al. 1987

Arthritis & Rheumatism

View full text Add to dashboard Cite

We analyzed DNA restriction fragment length polymorphism (RFLP) in 53 white ankylosing spondylitis (AS) patients and 92 healthy controls, utilizing a full-length HLA-B7 complementary DNA probe. A 9.2-kilobase (kb) Pvu I1 fragment was found to be significantly increasexn frequency in B27 positive AS patients compared with the B27 positive control group (P = 0.00085, relative risk = 7.2). The presence of both B27 and the 9.2-kb RFLP in an individual conferred a relative risk for AS of 297, compared with a relative risk of 119 in those who had B27 alone. The 9.2-kb RFLP appears to be a marker for AS which, with HLA-B27, contributes further to susceptibility to the disease. Our The seronegative spondylarthropathies, including ankylosing spondylitis (AS), Reiter's syndrome, psoriatic arthritis/spondylitis, and the arthritidspondylitis of inflammatory bowel disease, constitute a heterogeneous group of disorders characterized by axial and/or peripheral arthritis, overlapping clinical features, a tendency toward familial aggregation, and the absence of rheumatoid factor or subcutaneous nodules (1). Although the association of AS and other seronegative spondylarthropathies with HLA-B27 is well documented (2,3), the precise role of B27 in the genetic susceptibility to AS is yet to be established. There is a marked discrepancy between the frequency of this antigen in the white population (6.8%) and the prevalence of AS (0.2%), which raises the possibility that a unique subtype of B27 or a linked gene might be more disease-specific. As many as 6 B27 subtypes have been identified by a combination of approaches, including monoclonal antibody studies (4), cytotoxic T lymphocyte studies (5,6), a restriction typing assay (7), high performance liquid chromatography peptide mapping @), and amino acid and nucleotide sequencing (9,lO). Different B27 variants have exhibited higher or lower prevalence according to ethnic group (1 1,12).The availability of complementary DNA (cDNA) probes specific for the class I major histocompatibility complex (MHC) region genes permits further definition of these genes at the DNA level. A useful approach in this regard has been the technique of restriction fragment length polymorphism (RFLP)

show abstract

“…The electrophoretic separation of the standard, Saccharomyces cerevis- iae YNN 295 (Bio-Rad), proved to be highly reproducible based on the actual and calculated DNA size and standard error of fit. Runs of several agarose inserts of the same strains and repeated separation of the same plugs resulted in mobilities within the deviation computed by the Duggelby method (9). Saccharomyces cerevisiae NRRL Y-2034 (Fig.…”

Section: Electrophoretic Parametersmentioning

confidence: 57%

Electrophoretic karyotyping of yeasts, and southern blotting using whole chromosomes as templates for the probe preparation.

Török

Royer

Rockhold

et al. 1992

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

A computer program for determining the size of DNA restriction fragments

Cited by 85 publications

References 7 publications

African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

Association of a 9.2‐kilobase pvu ii class i major histocompatibility complex restriction fragment length polymorphism with ankylosing spondylitis

Electrophoretic karyotyping of yeasts, and southern blotting using whole chromosomes as templates for the probe preparation.

Contact Info

Product

Resources

About