African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

Molecular epidemiology of African swine fever in East Africa

“…Virus clusters similar to those identified by restriction enzyme digestion analysis (Wesley and Pan, 1982;Blasco et al, 1989) were obtained when comparing different ASFV isolates on the basis of VP72 nucleotide sequences. The gene's proficiency in resolving virus relatedness was significant as its surface glycoproteins do not allow differentiation into serological types (Dixon et al, 2000).…”

Genetic Diversity of African Swine Fever Virus Isolates from Soft Ticks (Ornithodoros moubata) Inhabiting Warthog Burrows in Zambia

“…Virus clones differing in various properties including antigenic determinants, virulence, plaque size, haemadsorption characteristics and genome restriction enzyme profiles have been isolated from uncloned ASF virus isolates (Garcia-Barreno et al, 1986;Pan & Hess, 1985;Pan et al, 1988;Santurde et al, 1988;Wesley & Pan, 1982). In the present study our analysis was carried out on uncloned ASF virus isolated originally from ticks but subsequently grown in domestic pigs.…”

African Swine Fever Virus

“…The Vero cell-adapted ASFV strain BA71V became a prototype for molecular and genomic studies, although it was recognized that cell-culture-passaged viruses had genomic differences, expressed as restriction fragment differences, relative to field isolates of ASFV, some of which have genome lengths approaching 190 kbp (Blasco et al 1989a;Santurde et al 1988;Tabares et al 1987;Wesley and Pan 1982). Comparative restriction mapping studies revealed notable aspects of ASFV genomic variability.…”