A computer-assisted dual wavelength microphotometry system for the measurement of cytochrome P-450 content in sections.

Measurement of Antigen Content in Tissue Sections by Quantitative Light Microscopic Enzyme Immunohistochemistry.

“…When the method A is then combined with morphometry of endoplasmic reticulum (ER), the reductase density in ER membrane (number of reductase molecules per unit area of ER membrane) can be calculated (16). Recently, we also established a method for measuring the density of cytochrome P-450 molecules in ER by a combination of microphotometry of cytochrome P-450 (11,12,14,15,17) and morphometry of ER (2,3,14). When the cytochrome P-450 and the reductase densities thus obtained are combined, the resulting molar ratio is useful to study the interaction between cytochrome P-450 and its reductase molecules in ER membrane (16).…”