Measurement of Antigen Content in Tissue Sections by Quantitative Light Microscopic Enzyme Immunohistochemistry.

Abstract

In this paper, two quantitative light microscopic immunohistochemical methods are reported. One is the method for measuring an antigen content in tissue sections by a combination of enzyme immunohistochemistry with microphotometry and nitrocellulose (NC) binding assay. The NC model systems used in this method gave quantitative information on the relation between the amount of antigen and the intensity of immunostaining, and provided the apparent extinction coefficient. The other is the method using video-image processing. This method can measure an antigen content in sections rapidly and easily, even if the antigen could not be immobilized on NC membrane. These two methods are unable to measure all antigen contents in tissue sections.