A comprehensive strategy for the analysis of acoustic compressibility and optical deformability on single cells

Yang, Tianhang; Bragheri, Francesca; Nava, Giovanni; Chiodi, Ilaria; Mondello, Chiara; Osellame, Roberto; Berg-Sørensen, Kirstine; Cristiani, Ilaria; Minzioni, P.

doi:10.1038/srep23946

Cited by 29 publications

(32 citation statements)

References 55 publications

(76 reference statements)

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“…The cytoplasm consists of all the material inside of a living cell, with the exception of the nucleus [20]. With a bulk modulus of 4 TPa, the incompressible cytoplasm facilitates the cell's ability to elongate, but not to shrink [21]. This means that cells deform when subjected to compressive forces.…”

Section: Cellsmentioning

confidence: 99%

On the Behaviour of Living Cells under the Influence of Ultrasound

Rubin

Anderton

Smalberger

et al. 2018

Fluids

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Medical ultrasound technology is available, affordable, and non-invasive. It is used to detect, quantify, and heat tissue structures. This review article gives a concise overview of the types of behaviour that biological cells experience under the influence of ultrasound only, i.e., without the presence of microbubbles. The phenomena are discussed from a physics and engineering perspective. They include proliferation, translation, apoptosis, lysis, transient membrane permeation, and oscillation. The ultimate goal of cellular acoustics is the detection, quantification, manipulation and eradication of individual cells.

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Section: Cellsmentioning

confidence: 99%

On the Behaviour of Living Cells under the Influence of Ultrasound

Rubin

Anderton

Smalberger

et al. 2018

Fluids

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“…Gilpin et al measured the compressibility of C. elegans under the influence of a static pressure, giving the worms enough time to adapt to the external pressure changes. Similar to work on the acoustic compressibility on individual cancer cell lines (33), in our study we were interested in the acoustic compressibility in the MHz regime. At this frequency, the living cells or organisms are unable to physiologically or physically react and/or adapt by releasing water or changing osmolality.…”

Section: Resultsmentioning

confidence: 99%

Acoustic Compressibility of Caenorhabditis elegans

Baasch

Reichert

Lakämper

et al. 2018

Biophysical Journal

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The acoustic compressibility of Caenorhabditis elegans is a necessary parameter for further understanding the underlying physics of acoustic manipulation techniques of this widely used model organism in biological sciences. In this work, numerical simulations were combined with experimental trajectory velocimetry of L1 C. elegans larvae to estimate the acoustic compressibility of C. elegans. A method based on bulk acoustic wave acoustophoresis was used for trajectory velocimetry experiments in a microfluidic channel. The model-based data analysis took into account the different sizes and shapes of L1 C. elegans larvae (255 5 26 mm in length and 15 5 2 mm in diameter). Moreover, the top and bottom walls of the microfluidic channel were considered in the hydrodynamic drag coefficient calculations, for both the C. elegans and the calibration particles. The hydrodynamic interaction between the specimen and the channel walls was further minimized by acoustically levitating the C. elegans and the particles to the middle of the measurement channel. Our data suggest an acoustic compressibility k Ce of 430 TPa À1 with an uncertainty range of 520 TPa À1 for C. elegans, a much lower value than what was previously reported for adult C. elegans using static methods. Our estimated compressibility is consistent with the relative volume fraction of lipids and proteins that would mainly make up for the body of C. elegans. This work is a departing point for practical engineering and design criteria for integrated acoustofluidic devices for biological applications.

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“…Finally, a basic idea in the optofluidic field is that thanks to the different response exhibited by the samples under analysis to optical or acoustic forces it is also possible to use different actuation mechanisms as "sensing mechanisms", enabling for interesting selection and sorting strategies [18][19][20][21][22].…”

Section: Current Status and Challengesmentioning

confidence: 99%

Roadmap for optofluidics

Minzioni¹,

Osellame²,

Sada³

et al. 2017

J. Opt.

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Optofluidics, nominally the research area where optics and fluidics merge, is a relatively new research field and it is only in the last decade that there has been a large increase in the number of optofluidics applications as well as in the number of research groups devoted to the topic. Nowadays optofluidics applications include, without being limited to, lab-on-chip devices, fluid-based and controlled lenses, optical sensors for fluids and for suspended particles, biosensors, imaging tools, etc. The long list of potential optofluidics applications, which have been recently demonstrated, suggests that optofluidic technologies will become more and more common in everyday life in the future, causing a significant impact on many aspects of our society. A characteristic of this research field, deriving from both its inter-disciplinary origin and applications, is that in order to develop suitable solutions it is often required to combine a deep knowledge in different fields, ranging from materials science to photonics, from microfluidics to molecular biology and biophysics. As a direct consequence, also being able to understand the long-term evolution of optofluidics research is not an easy target. In this article we report several expert-contributions on different topics, so as to provide guidance for young scientists. At the same time we hope that this document will also prove useful for funding institutions and stake holders, to better understand the perspectives and opportunities offered by this research field.

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A comprehensive strategy for the analysis of acoustic compressibility and optical deformability on single cells

Cited by 29 publications

References 55 publications

On the Behaviour of Living Cells under the Influence of Ultrasound

On the Behaviour of Living Cells under the Influence of Ultrasound

Acoustic Compressibility of Caenorhabditis elegans

Roadmap for optofluidics

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