A comprehensive salivary analysis for oral cancer diagnosis

Shpitzer, Thomas; Bahar, Gideon; Feinmesser, Raphael; Nagler, Rafael M.

doi:10.1007/s00432-007-0207-z

Cited by 117 publications

(117 citation statements)

References 24 publications

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“…Also, significant difference was noted between healthy controls (group1) (63.04 mg/dl) and healthy tobacco users (group2) (231.67 mg/dl). A similar study was conducted by Shpitzer et al [28] and it was found that salivary LDH was significantly higher in the patients with OSCC compared to the control group (p<0.05). Nagler et al [29] in their study observed raised level in both pre-malignant and malignant oral mucosa pathologies.…”

Section: Variablessupporting

confidence: 49%

“…Joshi et al [30] in their study observed the mean lactate level in control, pre-malignant and OSCC group as 267.2 IU/L, 519.36 IU/L and 788.73 IU/ L respectively (p<0.01). Shpitzer et al [28] and Shpitzer et al [31] also found total salivary LDH levels to be high in oral cancer patients. Our results are also comparable with the study done by Shetty et al [32] who have reported consistently higher salivary LDH levels in oral precancer and cancer patients as compared to healthy controls.…”

Section: Variablesmentioning

confidence: 95%

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Utility Of Salivary CA-125 And LDH As Tumor Markers In Oral Malignancy

Yadav

Ja²,

Ar³

et al. 2018

IJCBR

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Background:The mortality and morbidity associated with oral Squamous cell carcinoma (OSCC) can be greatly reduced if tumor markers which can detect OSCC at an early stage are available. This study attempts to use saliva as the diagnostic medium to determine the correlation of salivary CA 125 and LDH in tobacco users with and without potentially malignant disorders. Changes in CA 125 and LDH level can be used as a marker in patients with and without premalignant disease and can be used as a deterrent in continuation of the habit. Aims: To assess the role of CA 125 & LDH as a tumour marker in Oral cancer patients. Materials and methods: Cross sectional observational study .Time period: December 2015 to August 2017.Study area: OPD of tertiary care hospital in pune. Total participants: 150 persons. Under non-stimulatory conditions whole saliva sample was collected from each individual and was centrifuged. The resulting supernatant was used for estimation of CA 125 and LDH levels. Results: In present study, mean Salivary LDH level was found to be lowest among cases of healthy individuals while it was highest among individuals who were diagnosed and underwent treatment for malignancy. However there was insignificant difference seen between levels of diagnosed case of oral malignancy and the ones who underwent treatment for oral malignancy. Also mean Salivary CA-125 was lowest in healthy individuals and highest in individuals diagnosed with oral malignancy and the levels were seen to be reduced significantly in the one who underwent treatment for oral malignancies than those who didn't. Conclusion: Salivary CA 125 and LDH has a role in early detection of oral malignancies and can be used as a diagnostic marker. However LDH levels are persistently raised even after treatment for oral malignancy.

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Section: Variablessupporting

confidence: 49%

Section: Variablesmentioning

confidence: 95%

Utility Of Salivary CA-125 And LDH As Tumor Markers In Oral Malignancy

Yadav

Ja²,

Ar³

et al. 2018

IJCBR

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“…The deregulation, mutation and over-expression of ubuquitin is reported in the cancer development, consequence to increase interactions of ubiquitin in the central cellular pathways that control signalling, cell cycle, endocytosis, cell death as well as interactions between pathways of the tumour and its surrounding tissue [36,37]. The increased interactions of the second hub protein, ALB, in cancer is not surprising as concentration of total ALB is known to associate with the development oral cancer [38,39]. The next hub protein in cancer, TSPO, is known to be highly regulated in normal oral tissues and a significant increase in the number of TSPO interactions in the Cancer network is supported by the fact that it is known to be over-expressed in a highly aggressive oral cancer tumour [40].…”

Section: Resultsmentioning

confidence: 99%

Dissortativity and duplications in oral cancer

et al. 2015

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More than 300,000 new cases worldwide are being diagnosed with oral cancer annually. Complexity of oral cancer renders designing drug targets very difficult. We analyse proteinprotein interaction network for the normal and oral cancer tissue and detect crucial changes in the structural properties of the networks in terms of the interactions of the hub proteins and the degree-degree correlations. Further analysis of the spectra of both the networks, while exhibiting universal statistical behavior, manifest distinction in terms of the zero degeneracy, providing insight to the complexity of the underlying system.Oral cancer refers to a subgroup of head and neck malignancies that develop at the lips, tongue, salivary glands, gingiva, floor of the mouth, oropharynx, buccal surfaces and other intra-oral locations according to the international classification of diseases [1]. More than 300,000 new cases worldwide are being diagnosed with Oral squamous cell carcinoma (OSCC) annually [2]. WHO estimates it particularly to be the eighth most common cancer worldwide [3] and it is of significant public health importance to developing countries such as in Indian subcontinent where it ranks among the top three types of cancer and accounts for over 30% of all cancers [4][5][6]. Despite the growing propensity for oral cancer, majority of the research works have focused only on breast cancer [7], colon cancer [8] and lymphomas [9,10]. Hence, the identification of genetic changes specific to oral cancer is very crucial because it provides an opportunity to use this information for the development of drugs to treat this disease [11]. Availability of huge amount of proteomic data [12] and information on the highly interlinked internal organization of the cell metabolism, signal transduction, transport etc.[13], provides us a scope to identify and understand, the principles that govern the behaviour of cells. We analyse the proteomic data using the network theory framework [14,15] which is holistic approach that enables us to capture the intrinsic properties of the underlying system in a cost-effective and time-efficient manner.Previous attempts to understand various diseases under the network theory reveal that various types of cancers are interlinked through some pathways which are altered in different diseases [16]. This study explores the networks of the normal oral tissue and its diseasome, by characterizing their structural and spectral properties. This approach yields a comprehensive picture of the patterns and principles governing oral cancer which otherwise would not be apparent from the study of individual proteins [17]. We find significant variations between the structural and spectral properties of the Normal and Cancer network. The change in the degree-degree correlation provides quantitative information about the consequences of altered cell behaviour from the normal to cancer state. Further, the node duplication presents a clue to the abrupt transformation of the normal cells to the cancer cells which can be fu...

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“…MMP-9 and LDH have been measured quativavely in saliva of OSCC cancer patients whereas salivary carbonyls were studied by a western gel only. Studies of the other five markers have never been published in the professional literature Shpitzer et al, 2007). Furthermore, all eight markers have never been studied simultanously, nor have they been related to each other or evaluated for their diagnostic sensitivity and specificity values, or for their mutual pathogenetic role in OSCC.…”

mentioning

confidence: 99%

Salivary analysis of oral cancer biomarkers

et al. 2009

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BACKGROUND: Oral cancer is a common and lethal malignancy. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing. METHODS: We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress, DNA repair, carcinogenesis, metastasis and cellular proliferation and death. RESULTS: Five markers increased in cancer patients by 39 -246%: carbonyls, lactate dehydrogenase, metalloproteinase-9 (MMP-9), Ki67 and Cyclin D1 (CycD1) (Pp0.01). Three markers decreased by 16 -29%: 8-oxoguanine DNA glycosylase, phosphorylated-Src and mammary serine protease inhibitor (Maspin) (Pp0.01). Increase in salivary carbonyls was profound (by 246%, P ¼ 0.012); alterations in CycD1 (87% increase, P ¼ 0.000006) and Maspin (29% decrease, P ¼ 0.007) were especially significant. Sensitivity values of these eight analysed markers ranged from 58% to 100%; specificity values ranged from 42% to 100%. Both values were especially high for the CycD1 and Maspin markers, 100% for each value of each marker. These were also high for carbonyls, 90% and 80%, respectively, and for MMP-9, 100% and 79%, respectively. CONCLUSIONS: The significance of each salivary alteration is discussed. As all alterations correlated with each other, they may belong to a single carcinogenetic network. Cancer-related changes in salivary tumour markers may be used as a diagnostic tool for diagnosis, prognosis and post-operative monitoring.

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A comprehensive salivary analysis for oral cancer diagnosis

Cited by 117 publications

References 24 publications

Utility Of Salivary CA-125 And LDH As Tumor Markers In Oral Malignancy

Utility Of Salivary CA-125 And LDH As Tumor Markers In Oral Malignancy

Dissortativity and duplications in oral cancer

Salivary analysis of oral cancer biomarkers

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