A Comprehensive Review of Our Current Understanding of Red Blood Cell (RBC) Glycoproteins

Aoki, Takahiko

doi:10.3390/membranes7040056

Cited by 76 publications

(90 citation statements)

References 158 publications

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“…Moreover, while sialic acids are predominantly located on glycophorin A (~75 %), they are also located on band 3, glycolipids and other glycophorins of the cell membrane (de Oliveira and Saldanha, 2010), which may vary in junctional attachment sites. The findings of the present study, may suggest that the relative strength of the sialic acid junctional attachments, or the integrity of the O-linked and N-linked oligosaccharides containing sialic acid is varied (Aoki, 2017), with some attachments being more susceptible to shear.…”

Section: Accepted Manuscriptmentioning

confidence: 64%

Sublethal mechanical trauma alters the electrochemical properties and increases aggregation of erythrocytes

McNamee

Tansley

Simmonds

2018

Microvascular Research

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Circulation of blood depends, in part, on the ability of red blood cells (RBCs) to aggregate, disaggregate, and deform. The primary intrinsic disaggregating force of RBCs is derived from their electronegativity, which is largely determined by sialylated glycoproteins on the plasma membrane. Given supraphysiological shear exposure - even at levels below those which induce hemolysis - alters cell morphology, we hypothesized that exposure to supraphysiological and subhemolytic shear would cleave membrane-bound sialic acid, altering the electrochemical and physical properties of RBCs, and thus increase RBC aggregation. Isolated RBCs from healthy donors (n = 20) were suspended in polyvinylpyrrolidinone. Using a Poiseuille shearing system, RBC suspensions were exposed to 125 Pa for 1.5 s for three duty-cycles. Following the first and third shear duty-cycle, samples were assessed for: RBC aggregation; the ability of RBCs to aggregate independent of plasma ("aggregability"); disaggregation shear rate; membrane-bound sialic acid content, and; cell electrophoretic mobility. Initial shear exposure significantly increased RBC aggregation, aggregability, and the shear required for rouleaux dispersion. Sialic acid concentration significantly decreased on isolated RBC membranes ghosts, and increased in the supernatant following shear. Initial shear exposure decreased the electrophoretic mobility of RBCs, decreasing the electronegative charge from -15.78 ± 0.31 to -7.55 ± 0.21 mV. Three exposures to the shear duty-cycle did not further compound altered RBC measures. A single exposure to supraphysiological and subhemolytic shear significantly decreased the electrochemical charge of the RBC membrane, concurrently increasing cell aggregation/aggregability. The cascading implications of hyperaggregation appears to potentially explain the ischemia-associated complications commonly reported following mechanical circulatory support.

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Section: Accepted Manuscriptmentioning

confidence: 64%

Sublethal mechanical trauma alters the electrochemical properties and increases aggregation of erythrocytes

McNamee

Tansley

Simmonds

2018

Microvascular Research

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“…3 Figure 1 human red blood cell membranes. 2 From the diagram above, band 3 has one N-linked oligosaccharide, 10 while glycophorins possess mostly O-linked oligosaccharides. Sialic acid (otherwise called N-acetylneuraminic acid in the body of human) is seen at the extreme end of the O-linked oligosaccharide.…”

Section: Red Cell Membranementioning

confidence: 99%

“…The erythrocytes of humans, form the important cells generally employed in the investigation of biological membranes, have some glycoproteins in their cell membrane. 2 These membrane proteins are band 3 and glycophorins A-D, and some others glycoproteins. Glycophorin is a sialic acid conjugate with oligosaccharide which is rich in glycoproteins and it was detected by aid of staining with the periodic acid-Schiff's (PAS) reagent.…”

Section: Red Cell Membranementioning

confidence: 99%

“…Sialic acid (SA) bound to glycophorin A is responsible for the negative charge of the RBC membrane. 2…”

Section: Red Cell Membranementioning

confidence: 99%

“…Sialic acid has a terminal sugar in glycoconjugates. 2 Neuraminidase or sialidase enzyme cleaves sialic acid sugar moiety from selected glycoproteins (GP) and glycolipids (GL). The cleavage of GP or GL by NA promotes the release of trypanosome products into the cells.…”

Section: N-acetylneuraminic Acid (Sialic Acid or Neuraminic Acid) 11mentioning

confidence: 99%

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New insight into RBC membrane and trypanosome infection

Akinseye¹,

Mustapha²,

Moses³

2019

JAPLR

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Natural herbs and several synthetic drugs had been used to treat trypanosomiasis and are usually directed to kill the trypanosome. However, Red Blood cells (RBCs) membranes that is the first infection site for trypanosome remains defenseless to the invasion. Hence, there is need to protect the membrane against the invasion to limit the gravity of first acute parasitaemia before immune system is ready to defend the body against the parasites.

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Sialic acids rather than glycosaminoglycans affect normal and sickle red blood cell rheology by binding to four major sites on fibrinogen

et al. 2020

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A Comprehensive Review of Our Current Understanding of Red Blood Cell (RBC) Glycoproteins

Cited by 76 publications

References 158 publications

Sublethal mechanical trauma alters the electrochemical properties and increases aggregation of erythrocytes

Sublethal mechanical trauma alters the electrochemical properties and increases aggregation of erythrocytes

New insight into RBC membrane and trypanosome infection

Sialic acids rather than glycosaminoglycans affect normal and sickle red blood cell rheology by binding to four major sites on fibrinogen

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