A comprehensive review of CUPRAC methodology

Özyürek, Mustafa; Güçlü, Kubilay; Tütem, Esma; Başkan, Kevser Sözgen; Erçağ, Erol; Çelik, Saliha Esin; Baki, Sefa; Yıldız, Leyla; Karaman, Şeyda; Apak, Reşat

doi:10.1039/c1ay05320e

Cited by 154 publications

(89 citation statements)

References 39 publications

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“…7,8,[10][11][12][13][14][15][16] Solutions in the same conditions were studied by cyclic voltammetry and the voltammograms obtained are shown in Figure 2. In this case a reversible process, associated to the one electron reduction (peak Ic) of Cu(II) in solution.…”

Section: Spectroscopic and Electrochemical Cupracmentioning

confidence: 99%

Electrochemical Method to Quantify Antioxidants Employing Cupric Reducing Antioxidant Capacity, CUPRAC

Cárdenas¹,

Gómez²,

Frontana³

2014

Procedia Chemistry

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CUPRAC is a method used to determine antioxidants quantifying the chromophore [Cu(Nc) 2 ] + , generated upon increases of added antioxidants to a Cu(II) containing solution. In this work, an electrochemical alternative to quantify this complex is presented using cyclic voltammetry and chronoamperometry, compared with the classical spectroscopic determinations by UVVis. The final results show that the analysis performed by electrochemical methodologies is statistically similar, affording an efficient determination the total antioxidant capacity.

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Section: Spectroscopic and Electrochemical Cupracmentioning

confidence: 99%

Electrochemical Method to Quantify Antioxidants Employing Cupric Reducing Antioxidant Capacity, CUPRAC

Cárdenas¹,

Gómez²,

Frontana³

2014

Procedia Chemistry

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“…Both conceptual and technical problems limiting the use and validity of three commonly used [119] assays TEAC/ABTS*+, DPPH and ORAC have been subject of recent revision. Some reviews dealing with the DPPH [208,212,214], ORAC [223] and CUPRAC [239,240] assays have also been the subject of recent treatments. However, the aspects concerning with the assay chemistry, standardization and report of the antioxidants determination have not been solved after 25 years of intense study [199].…”

Section: Methods For Measuring the Antioxidant Activity Of Anthocyaninsmentioning

confidence: 99%

Antioxidant Capacity of Anthocyanin Pigments

Martín¹,

Kuskoski²,

Navas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Anthocyanins are a family of natural pigments classified into the group of flavonoids, considered to be responsible for the color and taste of many fruits and vegetables, i.e. berries. Anthocyanins are common components of the human diet. Besides their interest as colorant because of their coloring properties, the study of anthocyanin compounds stems from their wide applicability in the prevention and even in the treatment of various human diseases. However, various aspects of the pharmacological roles of anthocyanins remain in the dark, having still several obstacles to the development of robust diets or prescribing lines on consumption of anthocyanins. The chemical structure of anthocyanins determines in large measure its capacity and efficacy as an antioxidant agent. In this study, the following aspects are reviewed: the antioxidant effect of anthocyanin pigments; the oxidative stress, the bioavailability after intake and biological aspects of anthocyanins, the method for measuring the antioxidant activity of anthocyanins, the relationship between structure and activity; and the influence of the anthocyanins in the antioxidant activity of wines. Finally an overview of some potential uses in food industry is attempted mainly focusing in the anthocyanin encapsulation topic. Attention has been paid to the more recent publications in the field.

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“…CUPRAC is an electron transfer-based antioxidant quantification method that measures the capacity of an antioxidant by the reduction of Cu(II)-Nc. 57 DPPH assay also showed antioxidant activity of ND-COOH/eugenol, eugenol, and ND-COOH ( Figure 9C). All samples showed concentration-dependent antioxidant activity.…”

mentioning

confidence: 98%

Comprehensive evaluation of carboxylated nanodiamond as a topical drug delivery system

Lim¹,

Kim²,

Kang³

et al. 2016

IJN

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The best strategy in the development of topical drug delivery systems may be to facilitate the permeation of drugs without any harmful effects, while staying on the skin surface and maintaining stability of the system. Nanodiamonds (NDs) play a key role with their excellent physicochemical properties, including high biocompatibility, physical adsorption, reactive oxygen species (ROS) scavenging capability, and photostabilizing activity. Z -average sizes of carboxylated ND (ND–COOH) agglutinate decreased significantly as the pH increased. Fluorescein-conjugated ND was observed only on the stratum corneum, and no sample diffused into the dermal layer even after 48 hours. Moreover, ND–COOH and ND–COOH/eugenol complex did not show significant toxic effects on murine macrophage cells. ND improved in vitro skin permeation >50% acting as a “drug reservoir” to maintain a high drug concentration in the donor chamber, which was supported by quartz crystal microbalance results. Moreover, ND–COOH could adsorb a drug amount equivalent to 80% of its own weight. A photostability study showed that ND–COOH increased the photostability ~47% with regard to rate constant of the eugenol itself. A significant decrease in ROS was observed in the ND–COOH and ND–COOH/eugenol complex compared with the negative control during intracellular ROS assay. Moreover, ROS and cupric reducing antioxidant capacity evaluation showed that ND–COOH had synergistic effects of antioxidation with eugenol. Therefore, ND–COOH could be used as an excellent topical drug delivery system with improved permeability, higher stability, and minimized safety issue.

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A comprehensive review of CUPRAC methodology

Cited by 154 publications

References 39 publications

Electrochemical Method to Quantify Antioxidants Employing Cupric Reducing Antioxidant Capacity, CUPRAC

Electrochemical Method to Quantify Antioxidants Employing Cupric Reducing Antioxidant Capacity, CUPRAC

Antioxidant Capacity of Anthocyanin Pigments

Comprehensive evaluation of carboxylated nanodiamond as a topical drug delivery system

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