A comprehensive pilot plant system for fungal biomass protein production and wastewater reclamation

Jin, Bo; Yan, Xiaozhi; Qian, Yu; Leeuwen, J. Hans van

doi:10.1016/s1093-0191(01)00049-1

Cited by 67 publications

(54 citation statements)

References 8 publications

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“…Among 15 fungal strains tested, it had the highest protein content and shared the highest specific growth rate in starch processing wastewater, as well as having strong secreted amylase activity and a compact pellet morphology ideal for use in a bioreactor (14). In a pilot plant-scale air lift bioreactor, the strain efficiently converted carbon and other nutrients in starch processing wastewater into protein-rich fungal biomass (28). Yet little is known of the genetics of this strain.…”

Section: Discussionmentioning

confidence: 99%

Deletion of creB in Aspergillus oryzae Increases Secreted Hydrolytic Enzyme Activity

Hunter

Morris

Jin

et al. 2013

Appl Environ Microbiol

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dAspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic cycle. Phenotypes of an Aspergillus nidulans strain null for the CreB deubiquitinating enzyme include effects on growth and repression, including increased activity levels of various enzymes. We show that Aspergillus oryzae contains a functional homologue of the CreB deubiquitinating enzyme and that a null strain shows increased activity levels of industrially important secreted enzymes, including cellulases, xylanases, amylases, and proteases, as well as alleviated inhibition of spore germination on glucose medium. Reverse transcription-quantitative PCR (RTqPCR) analysis showed that the increased levels of enzyme activity in both Aspergillus nidulans and Aspergillus oryzae are mirrored at the transcript level, indicating transcriptional regulation. We report that Aspergillus oryzae DAR3699, originally isolated from soy fermentation, has a similar phenotype to that of a creB deletion mutant of the RIB40 strain, and it contains a mutation in the creB gene. Collectively, the results for Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, and Penicillium decumbens show that deletion of creB may be broadly useful in diverse fungi for increasing production of a variety of enzymes.A spergillus oryzae is a multicellular fungus that has been used for centuries for the production of Asian foods and beverages, including sake (rice wine) and shoyu (fermented soybean). Today it is also used industrially as a source of secreted enzymes, including cellulases, amylases, proteases, ␤-galactosidase, and lipase, and as a host for the production of heterologous proteins (1, 2).creB was identified in the model filamentous fungus Aspergillus nidulans in a screen for mutations that alleviate carbon catabolite repression (CCR) (3). CCR is a mechanism by which genes for the utilization of nonpreferred carbon sources are repressed in the presence of preferred carbon sources; organisms thus avoid wasting energy producing enzymes for the degradation of complex carbon sources when more readily metabolized carbon sources are available. A. nidulans creB mutants grow well and alleviate CCR of various enzymes, including acetyl-coenzyme A (acetyl-CoA) synthetase, isocitrate lyase, acetamidase, and alcohol dehydrogenase (3, 4). In addition, A. nidulans creB mutants show a pleiotropic range of phenotypes apparently unrelated to CCR, including slightly reduced conidiation, reduced growth with quinate, proline, or glucuronate as the sole carbon source, enhanced growth on acetamide or acrylamide, resistance to molybdate, hypersensitivity to acriflavine, and reduced acidification of liquid growth media (3-5). creB encodes a deubiquitinating enzyme (DUB); DUBs are cysteine proteases that specifically cleave ubiquitin from ubiqui...

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Section: Discussionmentioning

confidence: 99%

Deletion of creB in Aspergillus oryzae Increases Secreted Hydrolytic Enzyme Activity

Hunter

Morris

Jin

et al. 2013

Appl Environ Microbiol

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“…Fungi are often used in food industries as the sources of biomass and or by-products (Sankaran et al, 2010). Fungi can produce a wide range of chemicals and enzymes, and are more effective than bacteria in metabolizing complex carbohydrates such as starch and molasses (Hu et al, 2011;Jin et al, 1999aJin et al, , 2002. The fungal biomass can be used as a source of animal feed (Jin et al, 2000(Jin et al, , 2001Zhang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

Jin

Zepf

Zhang

et al. 2016

Process Safety and Environmental Protection

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Digestibility Fungi Protein enrichmentWinery biomass waste Solid state fermentation a b s t r a c t Grape marc and lees are main waste biomass in wine industry, which contain substantial amount of carbohydrates and nutrients, but currently deliver considerable carbon emission through landfill in Australia. This study was aimed to develop a biotechnological systematic approach to select suitable fungi for bioconversion of the winery biomass wastes into animal feed. The biotech-systematic approach was developed through assessment of nutrient/carbon source accessibility of the fungi, understanding of their metabolic reactions in solid state fermentation, and nutritive value of the fungi-fermented grape marc. From 13Aspergillus, Rhizopus, and Trichoderma fungal species, A. oryzae DAR 3699, A. oryzae RIB40 and T. reesei RUT C30 were determined as the best fungi due to their promising biochemical capabilities to enhance protein contents and in vitro digestibility of grape marc in mono-and co-cultivations. The mono-fungous-cultured SSF process used these selected fungi is able to convert grape marc to nutrient-rich feed by increasing the protein contents from ∼5% to 26% and the digestibility from ∼25% to over 50%. R. oligosporus 2710 with R. oryzae 6201 or T.viride 15719 showed promising protein enrichment to 18-23% and digestibility improvement by ∼50% in their co-cultivations. This preliminary bioengineering strategy will be useful for selecting microorganisms for the bioconversion of organic wastes to high valuable products.This "green cycle" bioprocess will be useful to promote the old-fashioned waste treatment technologies for the cleaner production in food processing industries.

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“…The MBP products could be used for human or animal consumption, which are marketable products and may offset the operating costs of the treatment process (Jin et al, 2002). Both fungi and yeast can be used to produce MBP.…”

Section: Introductionmentioning

confidence: 99%

“…Both fungi and yeast can be used to produce MBP. However, fungi seem more attractive because of the following advantages (Jin et al, 2002). Filamentous or pellet morphology of fungi permits low cost for separation and recovery of the MBP from the culture media, which makes up a significant fraction of the capital and operating costs for MBP production.…”

Section: Introductionmentioning

confidence: 99%

“…Fungi can be grown using almost any organic waste products that contain carbohydrates, such as confectionery and distillery waste, vegetable waste and wood processing effluents. Previous work focused on the production of MBP mainly using starch wastes (Jin et al, 1998(Jin et al, , 2002. However, the biotechnological treatment process integrated with fungal biomass production (FBP) from the winery waste streams was not reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Production of fungal biomass protein using microfungi from winery wastewater treatment

Zhang

Jin

Bai

et al. 2008

Bioresource Technology

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A comprehensive pilot plant system for fungal biomass protein production and wastewater reclamation

Cited by 67 publications

References 8 publications

Deletion of creB in Aspergillus oryzae Increases Secreted Hydrolytic Enzyme Activity

Deletion of creB in Aspergillus oryzae Increases Secreted Hydrolytic Enzyme Activity

A biotech-systematic approach to select fungi for bioconversion of winery biomass wastes to nutrient-rich feed

Production of fungal biomass protein using microfungi from winery wastewater treatment

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