A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis

Dema, Anusha; Tallapally, Mounika Reddy; Ganji, Vishweshwar Kumar; Buddala, Bhagyalakshmi; Kodi, Haritha; Ramidi, Ashwini; Yella, Narasimha Reddy; Putty, Kalyani

doi:10.1007/s00705-022-05674-6

Cited by 9 publications

(7 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Canine AstV has been reported to be associated with acute gastroenteritis or without clinical symptoms across the world. The only recently published study from the Hyderabad region of India reported a 7.2% prevalence of CAstV, and the strain with full genome sequence grouped with Chinese and Brazilian strains (Dema et al, 2023). In contrast, all the present study strains are grouped with a different cluster of Hungarian, Italian and Chinese strains.…”

Section: Discussioncontrasting

confidence: 58%

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

Sawant

Waghchaure

Shinde

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Astroviruses (AstV) and adenoviruses (AdV) are associated with diarrhoea in young ones of animals. However, the epidemiology and genetic diversity of AstVs and AdVs in animals are not well studied. Hence the present study was conducted to detect and characterize AstVs and AdVs in calves, piglets and puppies in India. Findings: Out of the processed porcine (48), canine (80), and bovine (65) faecal samples from Western Maharashtra, India, the porcine AstV (PAstV), bovine AstV (BAstV), canine AstV (CAstV), and porcine AdV (PAdV) were detected in 12.5%, 7.69%, 3.75% and 4.1% of samples, respectively. In the RNA-dependent RNA polymerase region-based phylogenetic analysis the detected BAstV strains grouped with MAstV-28, MAstV-33, and MAstV-35, CAstV strains belonged to MAstV-5, PAstV strains belonged to MAstV-24, MAstV-26, and MAstV-31. Both the detected PAdV were of genotype 3, exhibiting 91.9-92.5% nucleotide identity with Ivoirian and Chinese strains. The study reports first-time BAstVs from calves and PAdV-3 from piglets in India. Conclusion: The study revealed diversity in the circulation of AstVs and AdVs in young ones of dogs, pigs and calves. Therefore, extensive epidemiological investigations of AstV and AdV are necessary to confirm their association with diarrhoea and to develop diagnostic tools and control measures.

show abstract

Section: Discussioncontrasting

confidence: 58%

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

Sawant

Waghchaure

Shinde

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Since the exact role of CaAstV remains unknown, further observational studies or animal experiments are needed to better understand the pathogenic role of CaAstV. There are studies attempting in vitro isolation of CaAstV; the results have been either successful isolation ( 4 , 55 ) or unsuccessful isolation ( 3 , 39 ). Therefore, the cultivation of CaAstV remains a major challenge at present.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterization of canine astrovirus in non-diarrhea dogs and diarrhea dogs in Vietnam and Thailand reveals the presence of a unique lineage

Nguyen,

Piewbang,

Techangamsuwan

2023

Front. Vet. Sci.

View full text Add to dashboard Cite

The role of canine astrovirus (CaAstV) in canine gastrointestinal disease (GID) is unknown. In this study, a total of 327 fecal swab (FS) samples were collected, including 113 FSs in Vietnam (46 samples from healthy dogs and 67 samples from GID dogs) and 214 FSs in Thailand (107 samples from healthy dogs and 107 samples from GID dogs). Overall, the prevalence of CaAstV in Vietnam and Thailand was 25.7% (29/113) and 8.9% (19/214), respectively. CaAstV was detected in both non-diarrhea dogs (21.7 and 7.5%) and diarrhea dogs (28.4% and 10.3%), respectively, in Vietnam and Thailand. In both countries, CaAstV was frequently detected in puppies under 6 months of age (23.3%) (p = 0.02). CaAstV-positive samples in Vietnam and Thailand were identified as co-infected with canine parvovirus, canine enteric coronavirus, canine distemper virus, and canine kobuvirus. The complete coding sequence of seven Vietnamese CaAstV and two Thai CaAstV strains were successfully characterized. Phylogenetic analyses showed that Vietnamese and Thai CaAstV strains were genetically close to each other and related to the Chinese strains. Furthermore, analysis of complete coding sequences indicated that the OR220030_G21/Thailand/2021 strain formed a unique lineage, whereas no recombination event was found in this study, suggesting that this strain might be an original lineage. In summary, this is the first study to report the presence of CaAstV in dogs with and without diarrhea in Vietnam and Thailand, and it was most often found in puppies with diarrhea. Our results highlight the importance of the CaAstV in dog populations and the need for continued surveillance of these emerging pathogens.

show abstract

“…Canine AstV has been reported to be associated with acute gastroenteritis or without clinical symptoms across the world. The only recently published study from the Hyderabad region of India reported a 7.2% prevalence of CAstV, and the strain with full genome sequence grouped with Chinese and Brazilian strains [ 26 ]. In contrast, all the present study strains are grouped with a different cluster of Hungarian, Italian and Chinese strains.…”

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

Sawant,

Waghchaure,

Shinde

et al. 2023

Viruses

View full text Add to dashboard Cite

Astroviruses (AstV) and adenoviruses (AdV) are associated with diarrhoea in young animals. However, the epidemiology and genetic diversity of AstVs and AdVs in animals is not well studied. Hence, the present study was conducted to detect and characterize AstVs and AdVs in calves, piglets and puppies from Western Maharashtra, India. Out of the processed porcine (48), canine (80), and bovine (65) faecal samples, the porcine AstV (PAstV), bovine AstV (BAstV), canine AstV (CAstV), and porcine AdV (PAdV) were detected in 12.5%, 7.69%, 3.75% and 4.1% of samples, respectively. In the RNA-dependent RNA polymerase region-based phylogenetic analysis, the detected BAstV strains grouped with MAstV-28, MAstV-33, and MAstV-35, CAstV strains belonged to MAstV-5; PAstV strains belonged to MAstV-24, MAstV-26, and MAstV-31. However, in hexon gene-based phylogeny, both the detected PAdV were of genotype 3, exhibiting 91.9–92.5% nucleotide identity with Ivoirian and Chinese strains. The study reports first-time BAstVs from calves and PAdV-3 from piglets in India. The study revealed diversity in the circulation of AstVs in tested animals and AdVs in pigs, and suggested that they alone might be associated with other diarrhoea or in combination with other enteric pathogens, thus highlighting the necessity of extensive epidemiological investigations to develop diagnostic tools and control measures.

show abstract

A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis

Cited by 9 publications

References 58 publications

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

Genetic characterization of canine astrovirus in non-diarrhea dogs and diarrhea dogs in Vietnam and Thailand reveals the presence of a unique lineage

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

Contact Info

Product

Resources

About