A comprehensive method to elucidate pyoverdines produced by fluorescent Pseudomonas spp. by UHPLC-HR-MS/MS

Rehm, Karoline; Vollenweider, Vera; Kümmerli, Rolf; Bigler, Laurent

doi:10.1007/s00216-022-03907-w

Cited by 20 publications

(32 citation statements)

References 33 publications

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“…In this regard, Rehm and co-workers showed that searching for the ferribactin fragment ion turned out to be useful for the detection of several PVD variants from Pseudomonas spp., including unusual congeners bearing an isopyoverdine chromophore. Moreover, these authors developed a mass calculator and fragmentation predictor Excel tool for fast dereplication of PVDs, thus fostering discovery of novel derivatives by HR-MS based approaches [ 171 ].…”

Section: Analytical Methods For the Identification Of Pvdsmentioning

confidence: 99%

Novel Insights on Pyoverdine: From Biosynthesis to Biotechnological Application

Dell’Anno

Vitale

Buonocore

et al. 2022

IJMS

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Pyoverdines (PVDs) are a class of siderophores produced mostly by members of the genus Pseudomonas. Their primary function is to accumulate, mobilize, and transport iron necessary for cell metabolism. Moreover, PVDs also play a crucial role in microbes’ survival by mediating biofilm formation and virulence. In this review, we reorganize the information produced in recent years regarding PVDs biosynthesis and pathogenic mechanisms, since PVDs are extremely valuable compounds. Additionally, we summarize the therapeutic applications deriving from the PVDs’ use and focus on their role as therapeutic target themselves. We assess the current biotechnological applications of different sectors and evaluate the state-of-the-art technology relating to the use of synthetic biology tools for pathway engineering. Finally, we review the most recent methods and techniques capable of identifying such molecules in complex matrices for drug-discovery purposes.

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Section: Analytical Methods For the Identification Of Pvdsmentioning

confidence: 99%

Novel Insights on Pyoverdine: From Biosynthesis to Biotechnological Application

Dell’Anno

Vitale

Buonocore

et al. 2022

IJMS

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“…Furthermore, the pyoverdines of 13 Pseudomonas environmental isolates were investigated. They were sampled from soil and pond habitats on the campus of the University of Zurich Irchel (47.40° N, 8.54° E; Switzerland) (Butaite et al 2017 ) and their pyoverdine structures were elucidated in the work of Rehm et al ( 2022 ). A complete sample ID list is found in Table 1 stating the pyoverdine peptide chain sequences and their masses based on the pyoverdine derivative with a succinic acid side chain (Suc-Py) as recommended by Meyer et al ( 2008c ).…”

Section: Methodsmentioning

confidence: 99%

“…Hence, an additional fast way to screen for specific pyoverdine signals during the same analysis run is desirable. This can be achieved by all ion fragmentation as conducted in the studies of Wei and Aristilde (2015) or Rehm et al (2022). Pyoverdines and related compounds give characteristic fragments containing the chromophore unit making a rapid identification of precursor candidates possible.…”

Section: Screening For Characteristic Pyoverdine Fragments: Applicati...mentioning

confidence: 99%

“…Alternatively, pyoverdine precursor ions can be fragmented by MS/MS resulting in mainly B and Y″ (Y-ion plus two H atoms) ions. These fragments can be used for a total elucidation of the pyoverdine peptide chain (Budzikiewicz et al 2007b ; Wei and Aristilde 2015 ; Baune et al 2017 ; Rehm et al 2022 ). Such a structural elucidation is time-intensive and requires specialized expertise, whereas IEF and iron uptake experiments call for additional equipment that is not always accessible.…”

Section: Introductionmentioning

confidence: 99%

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Rapid identification of pyoverdines of fluorescent Pseudomonas spp. by UHPLC-IM-MS

et al. 2022

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Siderophores are iron-chelating molecules produced by bacteria and other microbes. They are involved with virulence in infections and play key roles in bacterial community assembly and as plant protectants due to their pathogen control properties. Although assays exist to screen whether newly isolated bacteria can produce siderophores, the chemical structures of many of these bio-active molecules remain unidentified due to the lack of rapid analytical procedures. An important group of siderophores are pyoverdines. They consist of a structurally diverse group of chromopeptides, whose amino acid sequence is characteristic for the fluorescent Pseudomonas species that secrets them. Although over 60 pyoverdine structures have been described so far, their characterization is cumbersome and several methods (isoelectrofocusing, iron uptake measurement, mass determination) are typically combined as ambiguous results are often achieved by a single method. Those additional experiments consume valuable time and resources and prevent high-throughput analysis. In this work, we present a new pyoverdine characterisation option by recording their collision cross sections (CCS) using trapped ion mobility spectrometry. This can be done simultaneously in combination with UHPLC and high-resolution MS resulting in a rapid identification of pyoverdines. The high specificity of CCS values is presented for 17 pyoverdines secreted by different Pseudomonas strains. The pyoverdine mass determination by full scan MS was supported by fragments obtained from broadband collision induced dissociation (bbCID). As iron contaminations in laboratories are not uncommon, CCS values of ferripyoverdines were also evaluated. Thereby, unusual and highly characteristic ion mobility patterns were obtained that are suitable as an alternative identification marker.

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“…Figure 3a shows that other secondary siderophores also occur in two forms: corrugatin vs. ornicorrugatin and quinolobactin vs. thioquinolobactin. Whether the chemical variation in these shows examples of peptide backbone variation found among P. aeruginosa strains (Schalk et al 2020) and among co-isolated natural strains (Rehm et al 2022). The blue box shows a list of side chain variants found among co-isolated natural strains (Rehm et al 2022) molecules also results in specificity, as shown for the pyochelin variants, remains to be tested.…”

Section: Iron Acquisition Through Siderophoresmentioning

confidence: 98%

Iron acquisition strategies in pseudomonads: mechanisms, ecology, and evolution

Kümmerli

2022

Biometals

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Iron is important for bacterial growth and survival, as it is a common co-factor in essential enzymes. Although iron is very abundant in the earth crust, its bioavailability is low in most habitats because ferric iron is largely insoluble under aerobic conditions and at neutral pH. Consequently, bacteria have evolved a plethora of mechanisms to solubilize and acquire iron from environmental and host stocks. In this review, I focus on Pseudomonas spp. and first present the main iron uptake mechanisms of this taxa, which involve the direct uptake of ferrous iron via importers, the production of iron-chelating siderophores, the exploitation of siderophores produced by other microbial species, and the use of iron-chelating compounds produced by plants and animals. In the second part of this review, I elaborate on how these mechanisms affect interactions between bacteria in microbial communities, and between bacteria and their hosts. This is important because Pseudomonas spp. live in diverse communities and certain iron-uptake strategies might have evolved not only to acquire this essential nutrient, but also to gain relative advantages over competitors in the race for iron. Thus, an integrative understanding of the mechanisms of iron acquisition and the eco-evolutionary dynamics they drive at the community level might prove most useful to understand why Pseudomonas spp., in particular, and many other bacterial species, in general, have evolved such diverse iron uptake repertoires.

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A comprehensive method to elucidate pyoverdines produced by fluorescent Pseudomonas spp. by UHPLC-HR-MS/MS

Cited by 20 publications

References 33 publications

Novel Insights on Pyoverdine: From Biosynthesis to Biotechnological Application

Novel Insights on Pyoverdine: From Biosynthesis to Biotechnological Application

Rapid identification of pyoverdines of fluorescent Pseudomonas spp. by UHPLC-IM-MS

Iron acquisition strategies in pseudomonads: mechanisms, ecology, and evolution

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