A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele

Ni, Kai; Steger, Klaus; Yang, Hao; Wang, Huanjiong; Hu, Kai; Zhang, T.; Chen, B.

doi:10.1111/andr.12210

Cited by 87 publications

(59 citation statements)

References 62 publications

(64 reference statements)

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“…Among the infertile group, patients with varicocele showed significantly higher DFI than did those without varicocele. This finding is in agreement with previous studies (Moazzam, Sharma, & Agarwal, ; Ni et al, ; Talebi, Moein, Tabibnejad, & Ghasemzadeh, ).…”

Section: Discussionsupporting

confidence: 94%

The correlation between mammalian target of rapamycin (mTOR) gene expression and sperm DNA damage among infertile patients with and without varicocele

et al. 2019

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This study aimed to assess the possible correlation between mammalian target of rapamycin (mTOR) gene expression and sperm DNA damage among infertile patients with and without varicocele. The study included sixty infertile males and fifty fertile males as controls. The infertile group was subdivided into the following subgroups: thirty males with varicocele and thirty males without varicocele. All subjects underwent medical history collection, clinical examination, semen analysis, sperm DNA integrity assessment, mTOR gene expression assessment and scrotal colour Doppler ultrasound. The mean mTOR gene expression in infertile patients with varicocele (23.52 ± 14.65) was significantly higher than that in infertile patients without varicocele (12.24 ± 12.44) and fertile control subjects (3.92 ± 3.26; p = 0.003 and p < 0.001 respectively). In the infertile varicocele‐positive group, mTOR gene expression showed a significant negative correlation with sperm count (p = 0.028, r = −0.400) and progressive sperm motility (p = 0.038, r = −0.381), as well as a significant positive correlation with the sperm DNA fragmentation index (DFI; p = 0.001, r = 0.578). In the infertile varicocele‐negative group, mTOR gene expression showed a significant negative correlation with progressive sperm motility (p = 0.018, r = −0.429) and a significant positive correlation with sperm DFI (p < 0.001, r = 0.673). In conclusion, according to these results, there is a significant positive correlation between mTOR gene expression and sperm DFI among infertile patients with and without varicocele.

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Section: Discussionsupporting

confidence: 94%

The correlation between mammalian target of rapamycin (mTOR) gene expression and sperm DNA damage among infertile patients with and without varicocele

et al. 2019

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“…Previous studies demonstrated that heat-related oxidative stress or protamine deficiency in varicocele condition prone spermatozoa to DNA fragmentation (Bahreinian et al, 2015;Ni et al, 2016). In this study, we observed a significant reduction in percentage of residual histone in varicocelized rats that received Vit B+Vit E compared with varicocelized rats that received water at 4 months.…”

Section: As Shown Insupporting

confidence: 56%

The effect of vitamin E & vitamin B on sperm function in rat varicocele model

et al. 2019

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We assessed the effect of vitamin E and vitamin B (Vit E & Vit B) and their combination on sperm functional parameters in the rat varicocele model. Male rats (n = 120) were divided into control (n = 30), sham (n = 30) and varicocele induction (n = 60) groups. After 2 months, 10 rats from each group were sacrificed to verify varicocele model. This part of results showed that sperm parameters, DNA damage, lipid peroxidation and residual histone were adversely effected in the varicocele group. From the 50 remaining rats in varicocele group, 10 rats received Vit B complex (6, 9.6, 30.4, 9.6 and 0.006 mg/kg for B6, B2, B9, B1 and B12 respectively), 10 rats received Vit E (40 mg/kg), 10 rats received Vit B & E, 10 rats only received water and 10 rats were only received sesame oil as a solvent for Vit E, for 2 months. From 40 remaining rats in control and sham groups, 20 rats only received water and other 20 rats only received sesame oil for 2 months. Then, all the aforementioned parameters were assessed. These results showed that Vit B antioxidant was more efficient in improvement of sperm parameters, chromatin integrity and lipid peroxidation in varicocelized rats compared with Vit E.

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“…In general, amongst the infertile groups, infertile patients with varicocele have a higher proportion of sperm with damaged DNA [27,34]. A recent study by Gual-Frau et al [27] showed that the integrity of sperm DNA in grade-I varicocele men can be improved by certain forms of oral multivitamin/antioxidant therapy, including vitamin B 12 .…”

Section: Effect Of Vitamin B12 On Sperm Parametersmentioning

confidence: 99%

Vitamin B12 and Semen Quality

Banihani

2017

Biomolecules

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Various studies have revealed the effects of vitamin B12, also named cobalamin, on semen quality and sperm physiology; however, these studies collectively are still unsummarized. Here, we systematically discuss and summarize the currently understood role of vitamin B12 on semen quality and sperm physiology. We searched the Web of Science, PubMed, and Scopus databases for only English language articles or abstracts from September 1961 to March 2017 (inclusive) using the key words “vitamin B12” and “cobalamin” versus “sperm”. Certain relevant references were included to support the empirical as well as the mechanistic discussions. In conclusion, the mainstream published work demonstrates the positive effects of vitamin B12 on semen quality: first, by increasing sperm count, and by enhancing sperm motility and reducing sperm DNA damage, though there are a few in vivo system studies that have deliberated some adverse effects. The beneficial effects of vitamin B12 on semen quality may be due to increased functionality of reproductive organs, decreased homocysteine toxicity, reduced amounts of generated nitric oxide, decreased levels of oxidative damage to sperm, reduced amount of energy produced by spermatozoa, decreased inflammation-induced semen impairment, and control of nuclear factor-κB activation. However, additional research, mainly clinical, is still needed to confirm these positive effects.

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A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele

Cited by 87 publications

References 62 publications

The correlation between mammalian target of rapamycin (mTOR) gene expression and sperm DNA damage among infertile patients with and without varicocele

The correlation between mammalian target of rapamycin (mTOR) gene expression and sperm DNA damage among infertile patients with and without varicocele

The effect of vitamin E & vitamin B on sperm function in rat varicocele model

Vitamin B12 and Semen Quality

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