A comprehensive genomic and growth proteomic analysis of antitumor lipopeptide bacillomycin Lb biosynthesis in Bacillus amyloliquefaciens X030

Lu, Jiao; Zhou, Ke; Huang, Weida; Zhou, Pengji; Yang, Shijia; Zhao, Xiaoli; Xie, Junyan; Xia, Liqiu

doi:10.1007/s00253-019-10019-6

Cited by 21 publications

(14 citation statements)

References 55 publications

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“…Finally, the ORFs c and b are predicted to be the putative homologs for the CoA transferase subunit A ( scoA / yxjD ) and B ( scoB / yxjE ). Although the subunits have been predicted to be involved in certain processes such as oxidative stress response [ 66 ], their role in the production of lipopeptide is more likely due to their significant homology towards the CoA transferases modules in the assembly lines of iturin [ 67 ] and bacillomycin [ 68 ]. The biosynthetic ORFs n and m are believed to encode for acyl-CoA synthetase and glycosyltransferase family 2 protein, respectively.…”

Section: Resultsmentioning

confidence: 99%

Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus

Habib

Ahmad

Johari

et al. 2020

IJMS

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Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research.

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Section: Resultsmentioning

confidence: 99%

Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus

Habib

Ahmad

Johari

et al. 2020

IJMS

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“…EPS from one of the endophytic B. amyloliquefaciens MD-b1 isolated from the Ophiopogon japonicas plant shows a high inhibitory effect on the gastric carcinoma cell lines of MC-4 and SGC-7901 cells . Biosynthesized lipopeptide bacillomycin Lb containing four major regulatory proteins (PerR, PhoP, CcpA, and CsfB) from B. amyloliquefaciens X030 shows high anticancer activity to SMMC-7721 and MDA-MB-231 cancer cells . Another study on the cell growth and application of l -asparagine depletion by asparaginase enzyme from B. amyloliquefaciens to treat patients with cancer is a potential strategy in increasing cell growth.…”

Section: Functional and Bioactive Applications Of B Amyloliquefaciensmentioning

confidence: 99%

Prebiotic, Probiotic, Antimicrobial, and Functional Food Applications of Bacillus amyloliquefaciens

Yohannes

Wan

et al. 2020

J. Agric. Food Chem.

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Bacillus amyloliquefaciens belongs to the genus Bacillus and family Baciliaceae. It is ubiquitously found in food, plants, animals, soil, and in different environments. In this review, the application of B. amyloliquefaciens in probiotic and prebiotic microbes in fermentation, synthesis, and hydrolysis of food compounds is discussed as well as further insights into its potential application and gaps. B. amyloliquefaciens is also a potential microbe in the synthesis of bioactive compounds including peptides and exopolysaccharides. In addition, it can synthesize antimicrobial compounds (e.g., Fengycin, and Bacillomycin Lb), which makes its novelty in the food sector greater. Moreover, it imparts and improves the functional, sensory, and shelf life of the end products. The hydrolysis of complex compounds including insoluble proteins, carbohydrates, fibers, hemicellulose, and lignans also shows that B. amyloliquefaciens is a multifunctional and potential microbe which can be applied in the food industry and in functional food processing.

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“…Until now, most of the researches are focused on the structure and function of lipopeptides and their potential applications in biomedical, agricultural, food and environmental aspects (31)(32)(33). Recently, more and more researches are shifting to improve the yield of lipopeptides by optimization of medium components, phenotypic dissociation, genome shuffling and related metabolic engineering (20,34,35).…”

Section: Purification and Identification Of Antifungal Agents Producementioning

confidence: 99%

Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1

Zhou

Liu

Zheng

et al. 2020

Appl Environ Microbiol

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In the present study, a deep-sea bacterial strain designated Bacillus sp. wsm-1 was screened to exhibit strong antifungal activity against many plant pathogenic fungi, and corresponding antifungal agents were thereby purified and determined by tandem mass spectrometry to be two cyclic lipopeptide homologs. These two lipopeptide homologs were identified to possess identical amino acid sequences of β-amino fatty acid-Asn-Ser-Asn-Pro-Tyr-Asn-Gln, which were different from any previously reported lipopeptides, and deduced as two novel lipopeptides designated as C14 iturin W and C15 iturin W, respectively. Electron microscopy observation indicated that both iturin W homologs caused obvious morphological changes and serious disruption of plasma membrane toward fungal cells, while C15 iturin W exhibited more serious cell damages than C14 iturin W did, which was consistent well with the results of the antifungal activity assays. To improve the yield and antifungal activity of iturin W, the effects of different carbon and nitrogen sources and amino acids on production of C14 iturin W and C15 iturin W were investigated, respectively. The results indicated that supplements of most of the detected carbon and nitrogen sources could increase the yield of C14 iturin W, but inhibit the yield of C15 iturin W, while supplements of tryptone and most of the detected amino acids could increase the yield of both C14 iturin W and C15 iturin W. IMPORTANCE Plant disease caused by pathogenic fungi is one of the most devastating diseases, which affects the food safety of the whole world to a great extent. Compared with traditional chemical control, biological control of plant diseases by microbial natural products is more desirable. In this study, we discovered a novel lipopeptide iturin W with promising prospect in biological control of plant diseases. Moreover, the effects of different carbon and nitrogen sources and amino acids on production of C14 iturin W and C15 iturin W would provide a reasonable basis for the optimization of fermentation process of lipopeptides. Notably, the structure of iturin W was different to any previously reported lipopeptides, suggesting deep-sea microorganisms might produce many novel natural products and have significant potentials to develop biological products in the future.

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A comprehensive genomic and growth proteomic analysis of antitumor lipopeptide bacillomycin Lb biosynthesis in Bacillus amyloliquefaciens X030

Cited by 21 publications

References 55 publications

Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus

Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus

Prebiotic, Probiotic, Antimicrobial, and Functional Food Applications of Bacillus amyloliquefaciens

Structural and Functional Insights into Iturin W, a Novel Lipopeptide Produced by the Deep-Sea Bacterium Bacillus sp. Strain wsm-1

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