A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco

Vandenabeele, Sophie; Kelen, Katrien Van Der; Dat, James F.; Gadjev, Ilya; Boonefaes, Tom; Morsa, Stijn; Rottiers, Pieter; Slooten, Luit; Montagu, Marc Van; Zabeau, Marc; Inzé, Dirk; Breusegem, Frank Van

doi:10.1073/pnas.2136610100

Cited by 296 publications

(223 citation statements)

References 55 publications

(55 reference statements)

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“…Our findings and model concerning the roles of RBOHD-derived ROS in the regulation of A. brassicicolainduced plant cell death represent a refinement of a concept suggested previously (Levine et al, 1994;Rao and Davis, 1999;Vandenabeele et al, 2003). ROS produced by plant cells under stress possess dual, concentration-dependent functions.…”

Section: Rbohd Interplays With Sa and Et In The Regulation Of Cell Deathsupporting

confidence: 69%

Dual Roles of Reactive Oxygen Species and NADPH Oxidase RBOHD in an Arabidopsis-Alternaria Pathosystem

et al. 2009

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Arabidopsis (Arabidopsis thaliana) NADPH oxidases have been reported to suppress the spread of pathogen- and salicylic acid-induced cell death. Here, we present dual roles of RBOHD (for respiratory burst oxidase homolog D) in an Arabidopsis-Alternaria pathosystem, suggesting either initiation or prevention of cell death dependent on the distance from pathogen attack. Our data demonstrate that a rbohD knockout mutant exhibits increased spread of cell death at the macroscopic level upon inoculation with the fungus Alternaria brassicicola. However, the cellular patterns of reactive oxygen species accumulation and cell death are fundamentally different in the AtrbohD mutant compared with the wild type. Functional RBOHD causes marked extracellular hydrogen peroxide accumulation as well as cell death in distinct, single cells of A. brassicicola-infected wild-type plants. This single cell response is missing in the AtrbohD mutant, where infection triggers spreading-type necrosis preceded by less distinct chloroplastic hydrogen peroxide accumulation in large clusters of cells. While the salicylic acid analog benzothiadiazole induces the action of RBOHD and the development of cell death in infected tissues, the ethylene inhibitor aminoethoxyvinylglycine inhibits cell death, indicating that both salicylic acid and ethylene positively regulate RBOHD and cell death. Moreover, A. brassicicola-infected AtrbohD plants hyperaccumulate ethylene and free salicylic acid compared with the wild type, suggesting negative feedback regulation of salicylic acid and ethylene by RBOHD. We propose that functional RBOHD triggers death in cells that are damaged by fungal infection but simultaneously inhibits death in neighboring cells through the suppression of free salicylic acid and ethylene levels.

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Section: Rbohd Interplays With Sa and Et In The Regulation Of Cell Deathsupporting

confidence: 69%

Dual Roles of Reactive Oxygen Species and NADPH Oxidase RBOHD in an Arabidopsis-Alternaria Pathosystem

et al. 2009

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“…RING fingers have E3 activity and the E3 enzymes are the determinants of the specificity for proteins subjected to proteasomal degradation. Substantiating these findings, a recent study on genes induced during H 2 O 2 -dependent cell death also found many members of the proteasome pathway upregulated, including regulatory and catalytic subunits as well as E2 and E3 enzymes [41]. The key to understanding the exact place of the plant proteasome in the cell death network will be identification of the potential proteasomal targets.…”

Section: Proteases and Aal-toxin-induced Cell Deathmentioning

confidence: 84%

“…The CONSTANS genes in Arabidopsis have an important role in the regulation of flowering by photoperiod [39,40]. A recent analysis of H 2 O 2 -induced cell death gene expression in tobacco also identified WRKY, NAM and MYB transcription factors as among the earliest genes induced [41]. Vandenabeele and co-workers also reported induction of SCARECROW transcription factors but not upregulation of C2H2-type zinc finger and DREB-like AP2 domain transcription factors nor downregulation of CONSTANS transcription factors.…”

Section: Transcription Factors and The Global Transcriptional Reprogrmentioning

confidence: 99%

“…Serine protease activity is required for apoptotic cell death in tobacco cell suspension cultures [45]. Moreover, plant subtilisin-like serine proteases may initiate signal transduction pathways by processing bioactive cell wall peptides [41]. In recent years, different components of the plant proteasome pathway have been implicated in processes requiring PCD, including catalytic or regulatory proteasomal subunits and components of the large family of E3 ubiquitin-protein ligases [10,46,47].…”

Section: Proteases and Aal-toxin-induced Cell Deathmentioning

confidence: 99%

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An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Gechev

Gadjev

Hille

2004

Cellular and Molecular Life Sciences (CMLS)

129

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An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants Gechev, T.S.; Gadjev, I.Z.; Hille, J. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. A T-DNA knockout of the Arabidopsis homologue of the tomato disease resistance gene Asc was obtained. The asc gene renders plants sensitive to programmed cell death (PCD) triggered by the fungal AAL toxin. To obtain more insights into the nature of AALtoxin-induced cell death and to identify genes of potential importance for PCD, we carried out transcription profiling of AAL-toxin-induced cell death in this knockout with an oligonucleotide array representing 21,500 Arabidopsis genes. Genes responsive to reactive oxygen species (ROS) and ethylene were among the earliest to be upregulated, suggesting that an oxidative burst and production of ethylene played a role in the activation of the cell death. This notion was corroborated by induction of several genes encoding ROS-generating proteins, including a respiratory burst oxidase and germin oxalate oxi- CMLS, Cell. Mol. Life Sci. 61 (2004) 1185 -1197 1420-682X/04/101185-13 DOI 10.1007/s00018-004-4067-2 © Birkhäuser Verlag, Basel, 2004 CMLS Cellular and Molecular Life Sciences dase. Cytochemical studies confirmed the oxidative burst and, in addition, showed synthesis of callose, a feature of the hypersensitive response. A diverse group of transcription factors was also induced. These events were followed by repression of most of the auxin-regulated genes known to be involved in growth and developmental responses. All photosynthesis-related genes were repressed. Blocking the synthesis of ethylene or NO significantly compromised cell death. In addition, we identified a heterogeneous group of early-induced genes, some of them never before associated with PCD. The group of earlyinduced genes included a number of proteases that were previously implicated in developmentally regulated types of PCD, suggesting a more principal role for these proteases in the PCD process. These findings provide new insights into the molecular mechanisms of plant PCD.

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“…Hydrogen peroxide is the most stable and longdistance ROS signaling molecule that regulates stress responses and developmental processes in plants (Vranová et al 2002). Vandenabeele et al (2002) showed that hydrogen peroxide could provoke transcriptional responses that mimicked both biotic and abiotic stresses. Therefore, sustained increases in H 2 O 2 content under rapid rehydration could also induce genes expression involved in physiological adjustments, e.g., the photosynthetic apparatus activity.…”

Section: (D)mentioning

confidence: 99%

Rapid plant rehydration initiates permanent and adverse changes in the photosynthetic apparatus of triticale

Hura

Ostrowska

et al. 2015

Plant Soil

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Background and aims The reasons for partial recovery after a soil drought are not fully understood and have not been studied so far. This study investigated the physiological and biochemical responses of triticale cultivars with differential recovery ability after soil water deficit. Methods Activity of the photosynthetic apparatus under soil drought followed by rehydration was estimated. Plant antioxidant potential was determined based on the measurement of catalase and peroxidase activity. The levels of hydrogen peroxide and superoxide radical were assessed. Results Under rehydration, the not fully-recovered cultivar experienced further significant increase in the content of H 2 O 2 and inhibited activity of the photosynthetic apparatus, as compared to the drought period. On 42 nd day of the rehydration, the not fully-recovered cultivar showed also a reduced photosynthetic activity in the flag leaves, which resulted in a significant decrease in its grain yield. The first week of a rapid rehydration involved a decrease in total peroxidase and catalase activities. The increased content of H 2 O 2 was compensated only when leaf water content was gradually restored in the first week of the rehydration and no further decrease in the activity of the photosynthetic apparatus was noticed. Conclusions A destructive effect of the rapid rehydration was manifested in an intensification of the physiological processes associated with reactive oxygen species (ROS) overproduction. An important cause of hydrogen peroxide overproduction seems to be the electron leakage due to overloading of the electron transport chain (ETC) in the PSI and PSII.

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A comprehensive analysis of hydrogen peroxide-induced gene expression in tobacco

Cited by 296 publications

References 55 publications

Dual Roles of Reactive Oxygen Species and NADPH Oxidase RBOHD in an Arabidopsis-Alternaria Pathosystem

Dual Roles of Reactive Oxygen Species and NADPH Oxidase RBOHD in an Arabidopsis-Alternaria Pathosystem

An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Rapid plant rehydration initiates permanent and adverse changes in the photosynthetic apparatus of triticale

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