A composite hydrogel with co-delivery of antimicrobial peptides and platelet-rich plasma to enhance healing of infected wounds in diabetes

Wei, Shikun; Xu, Pengcheng; Yao, Zexin; Chen, Xiao; Lei, Xiaoxuan; Li, Linlin; Dong, Yunqing; Zhu, Weidong; Guo, Rui; Cheng, Biao

doi:10.1016/j.actbio.2021.01.046

Cited by 166 publications

(144 citation statements)

References 57 publications

Supporting

Mentioning

138

Contrasting

Unclassified

Order By: Relevance

“…AMPs act by disorganizing bacterial cell membranes, adjusting the immune response, and moderating inflammation (Haney et al 2019). Accordingly, AMPs have been investigated for wound dressing aims due to antimicrobial and bioregulatory properties (Wei et al 2021). Hence, Fig.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of an Antimicrobial Peptide-Loaded Silk Fibroin/Gelatin Bilayer Sponge to Apply as a Wound Dressing; An In Vitro Study

Chizari

Khosravimelal

Tebyanian

et al. 2021

Int J Pept Res Ther

View full text Add to dashboard Cite

Recently, advanced biomaterial-based wound dressing due to bioactive properties have been considered and it has shown promising results. In this research, we prepared and improved an antibacterial silk fibroin/gelatin (SF/Gel) bilayer sponge scaffold loaded with various concentrations of a cationic antimicrobial peptide (CM11 peptide) as wound dressing for skin and soft tissue infections. The fabricated AMP-loaded SF/Gel sponge was completely designed and compared to the neat SF/Gel sponge in vitro. Then, the release behavior of various concentrations of peptide (8, 16, and 32 µg/ml) from the peptide-loaded SF/Gel sponge was analyzed. Eventually, according to cytotoxic activity, the antibacterial effect of scaffolds containing 8 and 16 µg/ml of the peptide was assessed against standard and multi-drug resistant strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa isolated from burn patients. The SF/Gel sponge loaded with peptide exhibited suitable mechanical attributes, high water uptake, optimum biodegradation rate, and a controlled release without cytotoxicity on Hu02 human foreskin fibroblast cells at the 8 and 16 µg/ml concentrations of peptide. The SF/Gel sponge including 16 µg/ml peptide represented a significant antibacterial effect against all strains. The results indicated that the antimicrobial modified sponge could be considered as a candidate to apply as a wound dressing.

show abstract

Section: Discussionmentioning

confidence: 99%

Fabrication of an Antimicrobial Peptide-Loaded Silk Fibroin/Gelatin Bilayer Sponge to Apply as a Wound Dressing; An In Vitro Study

Chizari

Khosravimelal

Tebyanian

et al. 2021

Int J Pept Res Ther

View full text Add to dashboard Cite

show abstract

“…Treatment of keratinocytes with 1,25-dihydroxyvitamin D3 induced cathelicidin and human β-defensin 2, increased keratinocyte migration, and showed effective antimicrobial activity [ 70 ]. Direct delivery of antimicrobial peptides using hydrogels [ 71 ], gold nanoparticles [ 72 ], and nanopolymers [ 73 ] showed antimicrobial activity and enhanced wound healing using mice and in vitro models.…”

Section: Pathophysiology Associated With Diabetic Wound Healingmentioning

confidence: 99%

Diabetic Wound-Healing Science

et al. 2021

View full text Add to dashboard Cite

Diabetes mellitus is an increasingly prevalent chronic metabolic disease characterized by prolonged hyperglycemia that leads to long-term health consequences. It is estimated that impaired healing of diabetic wounds affects approximately 25% of all patients with diabetes mellitus, often resulting in lower limb amputation, with subsequent high economic and psychosocial costs. The hyperglycemic environment promotes the formation of biofilms and makes diabetic wounds difficult to treat. In this review, we present updates regarding recent advances in our understanding of the pathophysiology of diabetic wounds focusing on impaired angiogenesis, neuropathy, sub-optimal chronic inflammatory response, barrier disruption, and subsequent polymicrobial infection, followed by current and future treatment strategies designed to tackle the various pathologies associated with diabetic wounds. Given the alarming increase in the prevalence of diabetes, and subsequently diabetic wounds, it is imperative that future treatment strategies target multiple causes of impaired healing in diabetic wounds.

show abstract

“…The CD31 and α-SMA immunofluorescence staining were shown in Figure 8 A,B. CD31 was highly expressed by endothelial cells and selected to characterize the newly-formed blood vessels, α-SMA was the actin isoform typical of smooth muscle cells and used to characterize the newly formed mature blood vessels [ 30 , 31 ]. The number of CD31 and α-SMA positive cells in the composite hydrogel-treated groups were significantly higher than the control group and GelMA group.…”

Section: Resultsmentioning

confidence: 99%

Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing

et al. 2021

View full text Add to dashboard Cite

Diabetic foot wound healing is a major clinical problem due to impaired angiogenesis and bacterial infection. Therefore, an effective regenerative dressing is desiderated with the function of promoting revascularization and anti-bacteria. Herein, a multifunctional injectable composite hydrogel was prepared by incorporation of the cerium-containing bioactive glass (Ce-BG) into Gelatin methacryloyl (GelMA) hydrogel. The Ce-BG was synthesized by combining sol-gel method with template method, which maintained spherical shape, chemical structure and phase constitution of bioactive glass (BG). The Ce-BG/GelMA hydrogels had good cytocompatibility, promoted endothelial cells migration and tube formation by releasing Si ion. In vitro antibacterial tests showed that 5 mol % CeO2-containing bioactive glass/GelMA (5/G) composite hydrogel exhibited excellent antibacterial properties. In vivo study demonstrated that the 5/G hydrogel could significantly improve wound healing in diabetic rats by accelerating the formation of granulation tissue, collagen deposition and angiogenesis. All in all, these results indicate that the 5/G hydrogel could enhance diabetic wound healing. Therefore, the development of multifunctional materials with antibacterial and angiogenic functions is of great significance to promote the repair of diabetic wound healing.

show abstract

A composite hydrogel with co-delivery of antimicrobial peptides and platelet-rich plasma to enhance healing of infected wounds in diabetes

Cited by 166 publications

References 57 publications

Fabrication of an Antimicrobial Peptide-Loaded Silk Fibroin/Gelatin Bilayer Sponge to Apply as a Wound Dressing; An In Vitro Study

Fabrication of an Antimicrobial Peptide-Loaded Silk Fibroin/Gelatin Bilayer Sponge to Apply as a Wound Dressing; An In Vitro Study

Diabetic Wound-Healing Science

Multifunctional Injectable Hydrogel Loaded with Cerium-Containing Bioactive Glass Nanoparticles for Diabetic Wound Healing

Contact Info

Product

Resources

About