A competitive microtitre plate enzyme immunoassay for plasma testosterone using polyclonal anti-testosterone immunoglobulins

Rajkowski, K.M.; Hanquez, Ch.; Bouzoumou, A.; Cittanova, N.

doi:10.1016/0009-8981(89)90335-5

Cited by 20 publications

(6 citation statements)

References 19 publications

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“…Various assays have been developed to measure testosterone in plasma (Rajkowski et al, 1989), serum (Dhar and Ali, 1992), and human urine (Al-Dujaili, 2006). The cumbersome handling procedures involved, hinder their use as on-site detection systems.…”

Section: Introductionmentioning

confidence: 99%

A recombinant Fab fragment-based electrochemical immunosensor for the determination of testosterone in bovine urine

Lu¹,

Kreuzer²,

Takkinen³

et al. 2007

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

A recombinant Fab fragment-based electrochemical immunosensor for the determination of testosterone in bovine urine

Lu¹,

Kreuzer²,

Takkinen³

et al. 2007

Biosensors and Bioelectronics

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“…whereθ is the estimate of the parameter vector from the training data,ŝ 2 calculated by equating each of the values in (4) to Y 0 and solving numerically for x 0 , for example, by using Brent's method [27]. The b-spline models cannot be easily inverted algebraically, so for them, we use Brent's method to obtain the point estimate as well as the confidence intervals.…”

Section: Cortisol Examplementioning

confidence: 99%

“…A common example of this is immunoassay, which uses an antibody to detect and quantitate the analyte . Immunoassays were originally developed for clinical use , but their use has spread to other areas including the analysis of food toxins and environmental contamination . In radioimmunoassay, the response is a radioactive count; most current applications use enzyme‐linked immunosorbent assay (ELISA) in which a color‐labeled enzyme produces an optical density.…”

Section: Introductionmentioning

confidence: 99%

A restricted b‐spline basis for S‐shaped calibration curves

Jones

Kawabata

2012

Journal of Chemometrics

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S‐shaped curves arise in many calibration assays. They are typically modeled using an empirically chosen nonlinear function. Nonparametric alternatives usually perform poorly because there are often insufficient data points and there are difficulties in enforcing the required asymptotic behavior. We propose an alternative approach using a b‐spline basis adapted to produce lower and upper asymptotes in the fitted curves. Its main advantage is that, with pre‐determined knot positions, the model is linear in the parameters and so is more numerically stable than a nonlinear model; this has advantages in, for example, fitting a mixed model to multiple curves. We explore the performance of this approach using data from a single radioimmunoassay for cortisol and from a set of 96 ELISA assays for the herbicide atrazine. Copyright © 2012 John Wiley & Sons, Ltd.

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“…Various methods have been developed to measure hormone levels in biological samples; immunoassay methods detecting T 2 in plasma, serum or urine(Hanquez et al , ; Rajkowski et al , ; Dhar and Ali, ; Al‐Dujaili, ); surface plasmon resonance, kinetic exclusion and ELISA method (Glass et al , ) for E 2 screening; liquid chromatography–electrospray ionization tandem mass spectrometry for T 2 and DHT in urine and prostate tissue samples (Yamashita et al , ). Electrochemical sensors were a better choice for the assay of hormones in biological samples, as a result of their low cost, time efficiency, easy sampling or no sampling (Goyal et al , ; Ojeda et al , ).…”

Section: Introductionmentioning

confidence: 99%

Multimode sensors as new tools for molecular recognition of testosterone, dihydrotestosterone and estradiol in children's saliva

Gugoaşă

Staden

Calenic

et al. 2015

J of Molecular Recognition

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Increased levels of testosterone (T2 ), dihydrotestosterone (DHT) and estradiol (E2 ) in children may be responsible for their early/delayed puberty and obesity conditions. Therefore, multimode sensors based on carbon matrices, such as graphite, graphene, fullerene C60 and multiwall carbon nanotubes modified with maltodextrin, were designed to assess reliably T2 , DHT and E2 in children saliva. The modes used for the assay of hormones were stochastic mode (for qualitative and quantitative determination of hormones) and differential pulse voltammetry mode (for quantitative determination of hormones). The advantage of this type of sensors, for hormone analysis, is their possibility to reach low concentration levels- are placed for children saliva under the detection limit of standard methods (e.g. ELISA used for the determination of these hormones in saliva). This made the multimode sensors an excellent tool for clinical analysis and especially for determination of substances of clinical importance in saliva samples. The proposed method is fast and simple, and no sampling of saliva is required.

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A competitive microtitre plate enzyme immunoassay for plasma testosterone using polyclonal anti-testosterone immunoglobulins

Cited by 20 publications

References 19 publications

A recombinant Fab fragment-based electrochemical immunosensor for the determination of testosterone in bovine urine

A recombinant Fab fragment-based electrochemical immunosensor for the determination of testosterone in bovine urine

A restricted b‐spline basis for S‐shaped calibration curves

Multimode sensors as new tools for molecular recognition of testosterone, dihydrotestosterone and estradiol in children's saliva

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