A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species

Kittigul, Leera; Singhaboot, Yutatirat; Chavalitshewinkoon-Petmitr, Porntip; Pombubpa, Kannika; Hirunpetcharat, Chakrit

doi:10.1016/j.fm.2014.07.020

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…Shellfish are filter feeders; for instance, oysters can circulate around 16 gallons of water per oyster/day, and the viral and bacterial concentrations in shellfish meat can reach 400 times higher than the levels in the growing water (49). HPP can be used to eliminate foodborne pathogens, such as bacteria and viruses, from oysters to enhance safety, reduce labor costs of shucking, and increase shelf life (17,18,21,43). Here, we found that HPP treatment at 400 MPa at an initial temperature of either 4 or 20°C was capable of eliminating 4.5 log 10 of RV strain Wa (the most stable RV strain) from the oyster tissues.…”

Section: Rv Is a Major Cause Of Infant Gastroenteritis And Death Worlmentioning

confidence: 99%

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High-Pressure Inactivation of Rotaviruses: Role of Treatment Temperature and Strain Diversity in Virus Inactivation

Araud

DiCaprio

Yang

et al. 2015

Appl Environ Microbiol

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c Rotavirus (RV) is the major etiological agent of acute gastroenteritis in infants worldwide. Although high-pressure processing (HPP) is a popular method to inactivate enteric pathogens in food, the sensitivity of different virus strains within same species and serotype to HPP is variable. This study aimed to compare the barosensitivities of seven RV strains derived from four serotypes (serotype G1, strains Wa, Ku, and K8; serotype G2, strain S2; serotype G3, strains SA-11 and YO; and serotype G4, strain ST3) following high-pressure treatment. RV strains showed various responses to HPP based on the initial temperature and had different inactivation profiles. Ku, K8, S2, SA-11, YO, and ST3 showed enhanced inactivation at 4°C compared to 20°C. In contrast, strain Wa was not significantly impacted by the initial treatment temperature. Within serotype G1, strain Wa was significantly (P < 0.05) more resistant to HPP than strains Ku and K8. Overall, the resistance of the human RV strains to HPP at 4°C can be ranked as Wa > Ku ‫؍‬ K8 > S2 > YO > ST3, and in terms of serotype the ranking is G1 > G2 > G3 > G4. In addition, pressure treatment of 400 MPa for 2 min was sufficient to eliminate the Wa strain, the most pressure-resistant RV, from oyster tissues. HPP disrupted virion structure but did not degrade viral protein or RNA, providing insight into the mechanism of viral inactivation by HPP. In conclusion, HPP is capable of inactivating RV at commercially acceptable pressures, and the efficacy of inactivation is strain dependent. R otavirus (RV) is the major etiological agent of acute gastroenteritis in infants worldwide (1, 2). RVs are estimated to cause nearly 500,000 deaths annually among children (3, 4). The virus is transmitted by the fecal-oral route, and contaminated water and food are common vehicles for infections (1,5,6). RV belongs to the genus Rotavirus, subfamily Sedoreovirinae, and family Reoviridae. There are eight species (groups) of rotavirus, referred to as A, B, C, D, E, F, G, and H. Humans are infected primarily by species A, B, and C, most commonly by species A. Rotavirus species A can be further divided into different serotypes. RV is a segmented double-stranded RNA virus with a triple-layer icosahedral capsid. The outer capsid glycoprotein (VP7) and the spike protein (VP4) differentiate RVs into 14 G (glycoprotein) serotypes and 27 different P (protease sensitivity) genotypes (1, 3, 4). Currently, five serotypes (G1 to G4 and G9) are the predominant circulating viruses, accounting for almost 95% of strains worldwide (1). Recently, commercial RV vaccines have been used in children to provide immunity against the most commonly circulating strains (4). Despite major efforts, RV outbreaks still occur worldwide due to the high genetic diversity of RVs and lack of cross-protection (2, 7-9). Therefore, alternative strategies for the prevention of RV infection must be established.Enteric viruses are a leading cause of foodborne illnesses. Within foodborne viruses, human norovirus (NoV), rotaviruses (RVs...

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Section: Rv Is a Major Cause Of Infant Gastroenteritis And Death Worlmentioning

confidence: 99%

“…As a consequence, RVs are often found to contaminate bivalve shellfish (9,(14)(15)(16). In a survey of 300 shellfish (including oysters, mussels, and cockles) harvested in growing waters off the coast of Thailand, RV was detected in 8% of the samples (17). In a survey of oysters in Mexico City (n ϭ 30), 26.9% were found to contain RV (16).…”

mentioning

confidence: 99%

High-Pressure Inactivation of Rotaviruses: Role of Treatment Temperature and Strain Diversity in Virus Inactivation

Araud

DiCaprio

Yang

et al. 2015

Appl Environ Microbiol

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“…Major viruses causing gastroenteritis in humans through food consumption include the norovirus (NoV) GI and GII genogroups, hepatitis a virus (HAV), astrovirus (AstV), and rotavirus (RoV) [2,3]. Representative viralcontaminated foods include water, shellfish (oysters), and fresh vegetables [4][5][6][7][8][9][10]. Prior reports of viral outbreaks from foods have inspired many additional studies related to viral contamination in foods [4,7,11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Representative viralcontaminated foods include water, shellfish (oysters), and fresh vegetables [4][5][6][7][8][9][10]. Prior reports of viral outbreaks from foods have inspired many additional studies related to viral contamination in foods [4,7,11,12]. Several common detection methods for foodborne viruses include electron microscopy (EM) examination, virus isolation techniques, the enzyme-linked immunosorbent assay (ELISA), and molecular methodologies such as dot blot hybridization and reverse transcription PCR (RT-PCR) [13,14].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR

Lee

Kim

et al. 2018

Journal of Microbiology and Biotechnology

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A one-step multiplex reverse transcription PCR (RT-PCR) method comprising six primer sets (for the detection of norovirus GI and GII, hepatitis A virus, rotavirus, and astrovirus) was developed to simultaneously detect four kinds of pathogenic viruses. The size of the PCR products for norovirus GI and GII, hepatitis A virus (VP3/VP1 and P2A regions), rotavirus, and astrovirus were 330, 164, 244, 198, 629, and 449 bp, respectively. The RT-PCR with the six primer sets showed specificity for the pathogenic viruses. The detection limit of the developed multiplex RT-PCR, as evaluated using serially diluted viral RNAs, was comparable to that of one-step single RT-PCR. Moreover, this multiplex RT-PCR was evaluated using food samples such as water, oysters, lettuce, and vegetable product. These food samples were artificially spiked with the four kinds of viruses in diverse combinations, and the spiked viruses in all food samples were detected successfully.

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“…(FELDHUSEN, 2000;DIEGO et al, 2013). Os vírus são: adenovírus (KARAMOKO et al, 2005;RIGOTTO et al, 2005), NoV (NISHIDA et al, 2007TERIO et al, 2010;CAMPOS et al, 2015;WOODS et al, 2016), rotavírus (RoV) (BAGORDO et al, 2013;KITTIGUL et al, 2015), vírus da hepatite E (LEES, 2000) e VHA (ATMAR et al, 1995;POLO et al, 2015). A Tabela 1 resume alguns agentes microbiológicos veiculados por moluscos bivalves e as principais caraterísticas das doenças transmitidas por alimentos.…”

Section: Contaminação Microbiológica Em Moluscos Bivalvesunclassified

Detecção de enterobactérias e vírus entéricos em frutos do mar no Estado de São Paulo

García¹

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Dedico aos meus queridos pais Doris e Arcesio, pelo amor e apoio incondicional, a minha irmã Gloria Lorena, pelo amor e amizade. A meu esposo Julian, pelo amor, paciência, carinho e dedicação em todos os momentos que passamos juntos nesta nossa conquista. Amo muito vocês....muito obrigada!! AGRADECIMENTOS A Deus, pela vida e a força espiritual que precisei para enfrentar cada uma das minhas decisões, meus objetivos e desafios durante esta longa jornada de formação acadêmica. A meus pais Doris Garcia e Arcesio Vasquez, por todo o amor, a força moral, pelo apoio contínuo e orientação nas decisões ao longo da minha vida. Obrigada pais, por estarem perto sem importar a distância. A minha irmã, Gloria Lorena Vasquez Garcia pela sua amizade única, por dar-me sempre uma voz de encorajamento e apoiar-me nas decisões que marcaram cada etapa de minha vida. Ao meu esposo, Julian Eduardo Mejia Ballesteros, por todo o amor que você dá para mim todos os dias, pela sua ajuda e conselhos e por ficar muito perto de mim.

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A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species

Cited by 18 publications

References 28 publications

High-Pressure Inactivation of Rotaviruses: Role of Treatment Temperature and Strain Diversity in Virus Inactivation

High-Pressure Inactivation of Rotaviruses: Role of Treatment Temperature and Strain Diversity in Virus Inactivation

Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR

Detecção de enterobactérias e vírus entéricos em frutos do mar no Estado de São Paulo

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