A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques

Travella, Silvia; Ross, S. M.; Harden, Jamie L.; Everett, Christopher J.; Snape, J. W.; Harwood, Wendy

doi:10.1007/s00299-004-0892-x

Cited by 228 publications

(132 citation statements)

References 43 publications

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“…Barley differs from rice and Arabidopsis in having two CO-like genes (HvCO1 and HvCO2) of which the former is unusual in having lost key residues in a normally highly conserved zinc-finger domain (B-box 2) (Griffiths et al 2003). Characterization of the roles and interactions of the various CO and FT genes in barley is now feasible because of the increased understanding of the gene families and the availability of new resources for functional analysis, including efficient transformation methods (Travella et al 2005) and a TILLING population (Caldwell et al 2004).…”

Section: Resultsmentioning

confidence: 99%

TheFLOWERING LOCUS T-Like Gene Family in Barley (Hordeum vulgare)

et al. 2007

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The FLOWERING LOCUS T (FT ) gene plays a central role in integrating flowering signals in Arabidopsis because its expression is regulated antagonistically by the photoperiod and vernalization pathways. FT belongs to a family of six genes characterized by a phosphatidylethanolamine-binding protein (PEBP) domain. In rice (Oryza sativa), 19 PEBP genes were previously described, 13 of which are FT-like genes. Five FT-like genes were found in barley (Hordeum vulgare). HvFT1, HvFT2, HvFT3, and HvFT4 were highly homologous to OsFTL2 (the Hd3a QTL), OsFTL1, OsFTL10, and OsFTL12, respectively, and this relationship was supported by comparative mapping. No rice equivalent was found for HvFT5. HvFT1 was highly expressed under long-day (inductive) conditions at the time of the morphological switch of the shoot apex from vegetative to reproductive growth. HvFT2 and HvFT4 were expressed later in development. HvFT1 was therefore identified as the main barley FT-like gene involved in the switch to flowering. Mapping of HvFT genes suggests that they provide important sources of flowering-time variation in barley. HvFTI was a candidate for VRN-H3, a dominant mutation giving precocious flowering, while HvFT3 was a candidate for Ppd-H2, a major QTL affecting flowering time in short days.

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Section: Resultsmentioning

confidence: 99%

TheFLOWERING LOCUS T-Like Gene Family in Barley (Hordeum vulgare)

et al. 2007

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“…One study comparing two different protoplast-based transformation techniques found that donor DNA underwent structural changes and concatemerisations in protoplasts transformed by a DNA coprecipitation technique, while donor DNA integrated by cocultivation of protoplasts with Agrobacterium was often unaltered (Czernilofsky et al 1986). Other studies comparing Agrobacterium-to biolistics-derived transgenic lines in cereals found transgene expression to be generally less stable in biolistics-derived lines (Dai et al 2001;Travella et al 2005) which were characterised as having more numerous, and mainly partial, transgene copies. These three studies imply that the specific treatment of donor DNA either prior to or during DNA uptake may affect the final integrity of the transgene DNA in situ, and thus its stability and expression to varying degrees in the subsequent transformant population.…”

Section: Discussionmentioning

confidence: 99%

Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts

Craig¹,

Gargano²,

Scotti³

et al. 2005

Plant Cell Rep

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Direct gene transfer methods in potato would facilitate the transfer of multiple genes and the manipulation of metabolic pathways in this species. In this study, up to 1.8 transformation events per shot (=0.5 per bombarded leaf) and 67.2 events per million protoplasts treated were obtained with particle bombardment and PEG-mediated direct DNA uptake, respectively. Limited disassociation of both HPT and GUS genes appeared to occur during the process of integration in only 19% of transformants. A large number of transformed potato plants with transgene expression at levels comparable to Agrobacterium-mediated transformation was obtained. High levels of GUS expression were only obtained in lines derived from PEG treatment. No correlation between the number of gene insertions and gene expression levels was found, suggesting that multiple insertions may have little or no effect on transgene expression.

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“…According to Travella et al (2005) and Li et al (2007), genetic transformation via particle bombardment presents some disadvantages, such as unstable inheritance and expression of the transgene, besides the possible integration of a high number of copies of the transgene in the plant genome. The present work differed from those in many aspects, because although our transgenic line was obtained via particle bombardment, only two copies of the transgene were integrated (as a single locus) in the plant genome, and the transgene inheritance followed the Mendelian pattern, with apparently stable expression, in all the crosses with commercial cultivars.…”

Section: Resultsmentioning

confidence: 99%

Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans

Pinheiro

Faria

Nogueira

et al. 2009

Pesq. agropec. bras.

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-The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specifi c oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close to 80% of the characteristics of the recurrent parent, as determined by the random amplifi ed DNA markers used, besides maintaining important traits such as resistance to BCMNV. The presence of the transgene did not cause any detectable undesirable effect in the evaluated progenies.Index terms: Phaseolus, BCMNV, genetic engineering, genetic variability, transgene genotypic stability. Herança de transgenes e similaridade genética de linhagens quase isogênicas de feijoeiro-comum geneticamente modifi cadoResumo -O objetivo do presente trabalho foi determinar a herança e a estabilidade de transgenes de uma linhagem de feijoeiro-comum com expressão dos genes rep-trap-ren, do Bean golden mosaic virus, e do gene bar. Foram realizados cruzamentos entre a linhagem transgênica e quatro cultivares comerciais de feijão, seguidos de quatro retrocruzamentos. As progênies de cada cruzamento foram avaliadas quanto à presença dos transgenes, com aplicação do glifosinato de amônia nas folhas e por meio da reação da polimerase em cadeia com uso de oligonucleotídeos específi cos. O vírus do mosaico comum necrótico do feijoeiro, Bean common mosaic necrosis virus (BCMNV), foi inoculado mecanicamente nas gerações avançadas. Os transgenes foram herdados em padrão mendeliano nos quatro cruzamentos estudados. As linhagens analisadas apresentaram cerca de 80% das características do parental recorrente, conforme determinado por análises com uso de marcadores de DNA, além de manter caracteres importantes, tais como resistência ao BCMNV. A presença do transgene não causou efeitos indesejáveis que pudessem ser detectados nas progênies avaliadas.Termos para indexação: Phaseolus, BCMNV, engenharia genética, variabilidade genética, estabilidade genotípica do transgene.

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A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques

Cited by 228 publications

References 43 publications

TheFLOWERING LOCUS T-Like Gene Family in Barley (Hordeum vulgare)

TheFLOWERING LOCUS T-Like Gene Family in Barley (Hordeum vulgare)

Direct gene transfer in potato: A comparison of particle bombardment of leaf explants and PEG-mediated transformation of protoplasts

Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans

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