A comparison of three guinea‐pig sensitization procedures for the detection of 19 reported human contact sensitizers

Goodwin, Benjamin; Crevel, René; Johnson, Anthony W.

doi:10.1111/j.1600-0536.1981.tb04060.x

Cited by 106 publications

(33 citation statements)

References 8 publications

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“…A chemical producing absolutely no response was classified as a nonsensitizer in the present GPMT. These results also correlated with those from previous studies [20, 21]. …”

Section: Discussionsupporting

confidence: 83%

“…In the present study, we could not detect benzocaine and cocoamidoproryl betain as a moderate sensitizer by using the GPMT. In some reports benzocaine and cocoamidoproryl betain was found to be a sensitizer in the GPMT [17, 19]; this sensitization potential was not confirmed in others, however [20, 21]. We do not know the reason for this discrepancy.…”

Section: Discussioncontrasting

confidence: 44%

See 1 more Smart Citation

Experimental Study for the Development of an in vitro Test for Contact Allergens

ARIMURA

Yokozeki

Katayama

et al. 1998

Int Arch Allergy Immunol

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We have previously reported an in vitro hapten-specific sensitization method using Pam-212 cells (in vitro sensitization test) to identify the potential effectiveness of contact allergens. In the present study, we conducted comparison studies of 11 allergens and 2 irritants in order to evaluate the method as an alternative predictive test. The guinea pig maximization test (GPMT) was developed based on the test described by Magnusson and Kligman. Our assay was carried out as follows: we treated Pam-212 cells with 13 test chemical solutions, while T cells and macrophages of BALB/c mice were cultured with hapten-conjugated Pam-212 cells for 5 days. After incubation, 10⁵ T cells were stimulated with mitomycin-C-treated spleen cells conjugated with chemicals. Three days later, the [³H]methyl thymidine incorporation was counted. The results of the GPMT were in agreement with those reported in previous studies except for benzocaine. In our GPMT experiments, benzocaine was negative, but it had been classified as a moderate sensitizer in previous studies. Our assay detected extreme, strong and moderate sensitizers as previously classified by the GPMT. They could be summarized as follows: three of five chemicals classified as moderate sensitizers, and 100% of strong or extreme sensitizers were detected by both the GPMT and the in vitro sensitization test. No irritants showed a positive reaction in our assay. These results support the view that the sensitivity of our in vitro test may be equivalent to that of the GPMT and may be useful as a rapid and objective allergen screening test.

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“…A chemical producing absolutely no response was classified as a nonsensitizer in the present GPMT. These results also correlated with those from previous studies [20, 21]. …”

Section: Discussionsupporting

confidence: 83%

Section: Discussioncontrasting

confidence: 44%

Experimental Study for the Development of an in vitro Test for Contact Allergens

ARIMURA

Yokozeki

Katayama

et al. 1998

Int Arch Allergy Immunol

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“…Most authors found their own method comparable to other test methods, when they examined various ranges of chemicals (Klecak et al 1977;Turk & Parker 1977;Maurer et al 1978Maurer et al & 1980Kero & Hannuksela 1980;Goodwin et al 1981;Guillot et al 1983;Klecak 1987;Marzulli & Maguire 1987). Table 3 shows selected comparative test results.…”

Section: Which Test Should Be Used?mentioning

confidence: 92%

Testing for Contact Allergy in Experimental Animals

Andersen

1987

Pharmacology & Toxicology

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“…Guinea pigs were tested in groups of 10 in various test systems: marked sensitization was seen in 100 % of the animals tested in a modified Draize test and in the single injection adjuvant test and 90 % of those tested in the maximization test [85].…”

Section: Allergenic Effectsmentioning

confidence: 99%

p‐Phenylenediamine [MAK Value Documentation, 1993]

2012

The MAK‐Collection for Occupational Health and Safety

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Published in the series Occupational Toxicants , Vol. 6 (1993) The article contains sections titled: Toxic Effects and Modes of Action Pharmacokinetics Effects in Man Acute toxicity Effects on skin and mucous membranes Allergenic effects Chronic toxicity Effects on Animals Acute toxicity Effects on skin and mucous membranes Allergenic effects Chronic Toxicity Reproductive and Developmental Toxicity Genotoxicity Carcinogenicity Manifesto (MAK value, classification)

show abstract

A comparison of three guinea‐pig sensitization procedures for the detection of 19 reported human contact sensitizers

Cited by 106 publications

References 8 publications

Experimental Study for the Development of an in vitro Test for Contact Allergens

Experimental Study for the Development of an in vitro Test for Contact Allergens

Testing for Contact Allergy in Experimental Animals

p‐Phenylenediamine [MAK Value Documentation, 1993]

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