Testing for Contact Allergy in Experimental Animals

Andersen, Klaus Ejner

doi:10.1111/j.1600-0773.1987.tb01762.x

Cited by 19 publications

(8 citation statements)

References 50 publications

(34 reference statements)

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“…A similar (non-linear dose-response) phenomenon is well-described for contact sensitization to many other reactive chemicals, e.g. formaldehyde, picryl chloride, DNCB [47]. …”

Section: Resultsmentioning

confidence: 76%

Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses

Wisnewski

Robinson

et al. 2011

Journal of Occupational Medicine and Toxicology

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BackgroundMethylene diphenyl diisocyanate (MDI), a reactive chemical used for commercial polyurethane production, is a well-recognized cause of occupational asthma. The major focus of disease prevention efforts to date has been respiratory tract exposure; however, skin exposure may also be an important route for inducing immune sensitization, which may promote subsequent airway inflammatory responses. We developed a murine model to investigate pathogenic mechanisms by which MDI skin exposure might promote subsequent immune responses, including respiratory tract inflammation.MethodsMice exposed via the skin to varying doses (0.1-10% w/v) of MDI diluted in acetone/olive oil were subsequently evaluated for MDI immune sensitization. Serum levels of MDI-specific IgG and IgE were measured by enzyme-linked immunosorbant assay (ELISA), while respiratory tract inflammation, induced by intranasal delivery of MDI-mouse albumin conjugates, was evaluated based on bronchoalveolar lavage (BAL). Autologous serum IgG from "skin only" exposed mice was used to detect and guide the purification/identification of skin proteins antigenically modified by MDI exposure in vivo.ResultsSkin exposure to MDI resulted in specific antibody production and promoted subsequent respiratory tract inflammation in animals challenged intranasally with MDI-mouse albumin conjugates. The degree of (secondary) respiratory tract inflammation and eosinophilia depended upon the (primary) skin exposure dose, and was maximal in mice exposed to 1% MDI, but paradoxically limited in mice receiving 10-fold higher doses (e.g. 10% MDI). The major antigenically-modified protein at the local MDI skin exposure site was identified as albumin, and demonstrated biophysical changes consistent with MDI conjugation.ConclusionsMDI skin exposure can induce MDI-specific immune sensitivity and promote subsequent respiratory tract inflammatory responses and thus, may play an important role in MDI asthma pathogenesis. MDI conjugation and antigenic modification of albumin at local (skin/respiratory tract) exposure sites may represent the common antigenic link connecting skin exposure to subsequent respiratory tract inflammation.

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“…A similar (non-linear dose-response) phenomenon is well-described for contact sensitization to many other reactive chemicals, e.g. formaldehyde, picryl chloride, DNCB [47]. …”

Section: Resultsmentioning

confidence: 76%

Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses

Wisnewski

Robinson

et al. 2011

Journal of Occupational Medicine and Toxicology

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“…Sensitization methods involving Freund's com plete adjuvant (FCA) injections generally yield the best results for nickel and chromium [2,[4][5][6], whereas repeated open epicutaneous (e.c.) sensitization ap parently is more effective in mercury ACD [5,7].…”

Section: Introductionmentioning

confidence: 99%

Successful Induction of Allergic Contact Dermatitis to Mercury and Chromium in Mice

et al. 1991

Int Arch Allergy Immunol

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Availability of reproducible mouse models for allergic contact dermatitis (ACD) to the metal allergens nickel, mercury and chromium, would be of great value for pathogenetic and preventive studies. We explored epicutaneous sensitization to nickel, mercury and chromium in mice in which oral grooming of the sensitization site was prevented by a plaster cast around the abdomen and lower thorax. This procedure was based on earlier findings that oral ingestion of allergen could prevent contact sensitization. The present results show that BALB/c mice can be readily sensitized to mercury and chromium using this epicutaneous casting method, without the further use of adjuvants. With nickel, however, neither this method, nor conventional methods involving the use of Freund’s complete adjuvant (FCA) were effective.

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“…The method has pfeviously been applied to other dental materials, e.g., endodontie sealers (21), denture base rnaterials (22), and resin-based filling materials (23). Ideally, in the guinea pig maximization test, the animals are exposed to well-deftned chemical compounds (24). However, previous experietice with guitiea pigs with endodotitic sealers based on zinc oxide-eugenol, colophony, or epoxy resitis (21) has itidicated that aqueous extracts of the materials are capable of sensitizing by this method, and that the sensitization rate correlates with the known sensitizing potential of some of the ingredients of the material.…”

Section: Discussionmentioning

confidence: 99%

Allergenic potential of two orthodontic bonding materials

Söhoel

Gjerdet

Hensten‐Pettersen

et al. 1994

European J Oral Sciences

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The sensitization potential of two orthodontic bonding materials, one conventional premix product and one nonmix product, was evaluated by a modified guinea pig maximization test. The nonmix product, consisting of a liquid to be applied to the tooth surface and a paste which was placed on the bracket base, caused reaction in 50% of the tested animals. This material should be considered to be a possible cause of allergy in patients or professionals handling it.

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Testing for Contact Allergy in Experimental Animals

Cited by 19 publications

References 50 publications

Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses

Immune sensitization to methylene diphenyl diisocyanate (MDI) resulting from skin exposure: albumin as a carrier protein connecting skin exposure to subsequent respiratory responses

Successful Induction of Allergic Contact Dermatitis to Mercury and Chromium in Mice

Allergenic potential of two orthodontic bonding materials

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