A Comparison of Three Commercial Perfluorocarbon Emulsions as High-Field 19F NMR Probes of Oxygen Tension and Temperature

Shukla, H; Mason, Ralph P.; Woessner, D. E.; Antich, Peter P.

doi:10.1006/jmrb.1995.1023

Cited by 58 publications

(82 citation statements)

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“…29 resonances, respectively, were interrogated. The sample was then bubbled with a gas of known oxygen concentration for 30 min while the temperature was held constant.…”

Section: Pfc and Hmdso Mrs Calibrationmentioning

confidence: 99%

Development and Validation of Noninvasive Magnetic Resonance Relaxometry for the In Vivo Assessment of Tissue-Engineered Graft Oxygenation

Einstein

Weegman

Firpo

et al. 2016

Tissue Engineering Part C: Methods

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Techniques to monitor the oxygen partial pressure (pO 2 ) within implanted tissue-engineered grafts (TEGs) are critically necessary for TEG development, but current methods are invasive and inaccurate. In this study, we developed an accurate and noninvasive technique to monitor TEG pO 2 utilizing proton ( 1 H) or fluorine ( 19 F) magnetic resonance spectroscopy (MRS) relaxometry. The value of the spin-lattice relaxation rate constant (R 1 ) of some biocompatible compounds is sensitive to dissolved oxygen (and temperature), while insensitive to other external factors. Through this physical mechanism, MRS can measure the pO 2 of implanted TEGs. We evaluated six potential MRS pO 2 probes and measured their oxygen and temperature sensitivities and their intrinsic R 1 values at 16.4 T. Acellular TEGs were constructed by emulsifying porcine plasma with perfluoro-15-crown-5-ether, injecting the emulsion into a macroencapsulation device, and cross-linking the plasma with a thrombin solution. A multiparametric calibration equation containing R 1 , pO 2 , and temperature was empirically generated from MRS data and validated with fiber optic (FO) probes in vitro. TEGs were then implanted in a dorsal subcutaneous pocket in a murine model and evaluated with MRS up to 29 days postimplantation. R 1 measurements from the TEGs were converted to pO 2 values using the established calibration equation and these in vivo pO 2 measurements were simultaneously validated with FO probes. Additionally, MRS was used to detect increased pO 2 within implanted TEGs that received supplemental oxygen delivery. Finally, based on a comparison of our MRS data with previously reported data, ultra-high-field (16.4 T) is shown to have an advantage for measuring hypoxia with 19 F MRS. Results from this study show MRS relaxometry to be a precise, accurate, and noninvasive technique to monitor TEG pO 2 in vitro and in vivo.

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“…29 resonances, respectively, were interrogated. The sample was then bubbled with a gas of known oxygen concentration for 30 min while the temperature was held constant.…”

Section: Pfc and Hmdso Mrs Calibrationmentioning

confidence: 99%

Development and Validation of Noninvasive Magnetic Resonance Relaxometry for the In Vivo Assessment of Tissue-Engineered Graft Oxygenation

Einstein

Weegman

Firpo

et al. 2016

Tissue Engineering Part C: Methods

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“…T 1 relaxation measurements of these perfluorocarbons (with the CF 3 and the CF 2 resonance used for the PFTBA and PFCE, respectively) were made at four or more DO concentrations, ranging from 0 to 0.2 mM, using a standard inversion recovery pulse sequence provided by the Bruker operating software. The choice of resonances was based on literature reports demonstrating that they had the greatest sensitivity for their individual perfluorocarbon to changes in DO concentration (Shukla et al, 1995). For each T 1 measurement, 14 delay times were obtained ranging from 0.040s to 2 s. Additional key acquisition parameters were: sweep width ¼ 2.8 kHz, number of transients ¼ 1.…”

mentioning

confidence: 99%

Monitoring of dissolved oxygen and cellular bioenergetics within a pancreatic substitute

Gross

Long

Constantinidis

et al. 2007

Biotech & Bioengineering

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This work investigated the use of nuclear magnetic resonance (NMR) spectroscopy in combination with a mathematical model of an encapsulated cell system as a method for rapidly assessing the status of a pancreatic substitute. To validate this method, an in vitro experiment was performed in which the encapsulated cells were perfused in an NMR-compatible system and the dissolved oxygen (DO) concentration of the perfusing medium was lowered from 0.20 to 0.05 mM, then returned to 0.20 mM in a stepwise fashion. The cellular metabolic activity and bioenergetics were evaluated by measuring the oxygen consumption rate (via DO sensors) and nucleotide triphosphate levels (via (31)P NMR). By incorporating a perfluorocarbon emulsion into the alginate beads, the cellular oxygenation state was monitored by measuring the average intrabead DO (AIDO) concentration by (19)F NMR. The in vitro measurements were then compared with model predictions based on the measured external DO concentration and time. Model-predicted cell growth and AIDO closely matched the experimentally acquired data. As the DO concentrations both external to and within the pancreatic substitute are needed to apply this methodology in vivo, the feasibility of measuring the DO concentration from two distinct bead populations implanted in the peritoneal cavity of mice was established. It is concluded that PFC incorporation and (19)F NMR measurements, in combination with a mechanistic model of the encapsulated system, allow the tracking of the state of a pancreatic substitute in vitro and potentially in vivo.

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“…The temperature dependence of these relaxation rates can reveal information of the molecular dynamics that dominate these relaxation processes. In case of both anoxic and oxic PFTB the R 1 maxima were observed to occur at similar temperatures (91). This implies that in this case the τ c that determine R 1p and R 1d are approximately equal.…”

Section: Paramagnetic Contribution Of Oxygenmentioning

confidence: 69%

“…Due to the strong 1/r FF 6 dependence, the para and the meta contributions may be small (1/27 and 1/128 of the ortho contribution, respectively, accounting for the distances and number of atoms), but this has not been verified. ) resonances in PFTB (91,92). Of course, the differences in R 1d for different perfluorocarbons cannot be accounted for by considering the F-F distances only; molecular dynamics and CSA contributions play a major role as well.…”

Section: Diamagnetic Contributions To Rmentioning

confidence: 99%

Vatuximab(Trademark): Optimizing Therapeutic Strategies for Prostate Cancer Based on Dynamic MR Tumor Oximetry

Mason¹

2010

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A Comparison of Three Commercial Perfluorocarbon Emulsions as High-Field 19F NMR Probes of Oxygen Tension and Temperature

Cited by 58 publications

References 0 publications

Development and Validation of Noninvasive Magnetic Resonance Relaxometry for the In Vivo Assessment of Tissue-Engineered Graft Oxygenation

Development and Validation of Noninvasive Magnetic Resonance Relaxometry for the In Vivo Assessment of Tissue-Engineered Graft Oxygenation

Monitoring of dissolved oxygen and cellular bioenergetics within a pancreatic substitute

Vatuximab(Trademark): Optimizing Therapeutic Strategies for Prostate Cancer Based on Dynamic MR Tumor Oximetry

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