A Comparison of the Reproductive Physiology of Largemouth Bass, Micropterus salmoides, Collected from the Escambia and Blackwater Rivers in Florida

Orlando, Edward F.; Denslow, Nancy D.; Folmar, Leroy C.; Guillette, Louis J.

doi:10.2307/3434509

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…1 to 4 nM and from 5 to 10 nM respectively, depending on the reporter assay employed (Hall & McDonnell 1999. In general, fish have higher circulating concentrations of E 2 (600-3900 pg/ml for females (Orlando et al 1999, Sabo-Attwood et al 2004) in comparison with human females which range from 23 to 361 pg/ml depending on time of the menstrual cycle (during pregnancy, normal concentration can be as high as 35 000 pg/ml; Simpson & MacDonald 1981). Previous studies have shown that the rainbow trout ER binds E 2 with tenfold less affinity than hER, which may be a result of varied protein structure, and may explain why higher levels are noted in fish.…”

Section: Differential Activation Of Lmb Er Subtypes By E 2 and Npmentioning

confidence: 99%

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Sabo‐Attwood

Blum

Kroll

et al. 2007

Journal of Molecular Endocrinology

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The estrogen receptor (ER) signaling cascade is a vulnerable target of exposure to environmental xenoestrogens, like nonylphenol (NP), which are causally associated with impaired health status. However, the impact of xenoestrogens on the individual receptor isotypes (a, ba, and bb) is not well understood. The goal of these studies was to determine the impact of NP on largemouth bass (Micropterus salmoides) ER isotype expression and activity. Here, we show that hepatic expression levels of three receptors are not equivalent in male largemouth bass exposed to NP by injection. Transcript levels of the ERa subtype were predominantly induced in concert with vitellogenin similarly to fish exposed to 17b-estradiol (E 2 ) as measured by quantitative real-time PCR. NP also induced circulating plasma levels of estrogen, which may contribute to overall activation of the ERs. To measure the activation of each receptor isotype by E 2 and NP, we employed reporter assays using an estrogen response element (ERE)-luciferase construct. Results from these studies show that ERa had the greatest activity following exposure to E 2 and NP. This activity was inhibited by the antagonists ICI 182 780 and ZM 189 154. Furthermore, both bb and ba subtypes depressed ERa activation, suggesting that the cellular composition of receptor isotypes may contribute to the overall actions of estrogen and estrogenic contaminants via the receptors. Results from these studies collectively reveal the differential response of fish ER isotypes in response to xenoestrogens.

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Section: Differential Activation Of Lmb Er Subtypes By E 2 and Npmentioning

confidence: 99%

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Sabo‐Attwood

Blum

Kroll

et al. 2007

Journal of Molecular Endocrinology

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“…Many researches have been focused on fish, since as aquatic inhabitants they receive sewage or industrial effluent and agricultural runoff containing estrogenic chemicals [5,14,30]. In fish, estrogenic responses have been associated with exposure to pesticides [3,35], pulp mill effluents [23,29,34], plasticizers [19] and sewage effluent [2,17,31].…”

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confidence: 99%

Gonadosomatic Index and Testis Morphology of Common Carp (Cyprinus carpio) in Rivers Contaminated with Estrogenic Chemicals.

et al. 2002

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ABSTRACT. To study the effect of estrogenic chemicals on fish, the gonadosomatic index (GSI = [testis weight/body weight] × 100) and testis histology of mature common carp (Cyprinus carpio) from 2 contaminated sites (Ishizu and Wada rivers, Osaka) and a control site were examined between June 1998 and March 2001. The concentration of nonylphenol, bisphenol A and 17β-estradiol in the Ishizu river was 3-4 times higher than in the Wada river. In the pre-breeding and breeding seasons, there were no significant differences in body weight among carp from the 3 sites, the body weight of Ishizu river carp being significantly lower (p<0.05) than that of Wada river fish only in the post-breeding season. The GSI and testis weight in fish from the Ishizu river were significantly lower (p<0.05) than in control fish during all phases of gonadal cycle and lower than in Wada river fish in the pre-breeding and post-breeding season. No histological abnormalities were found in the testes of the males examined. Histological observation of the testes revealed a delay in the onset of spermatogenesis in fish from the Ishizu river compared with those from the other sites. These results clearly imply that the estrogenic chemicals in the Ishizu river adversely affect the testis development of the fish. There is growing evidence in support of the claim that contamination of a wildlife population with estrogenic chemicals can disturb the reproductive function of vertebrates [6,7,12]. Many researches have been focused on fish, since as aquatic inhabitants they receive sewage or industrial effluent and agricultural runoff containing estrogenic chemicals [5,14,30]. In fish, estrogenic responses have been associated with exposure to pesticides [3,35], pulp mill effluents [23,29,34], plasticizers [19] and sewage effluent [2,17,31]. Abnormalities have also been reported in the male reproductive system of fish living in water polluted by sewage or industrial chemicals [12,25]. Studies on the male teleost revealed changes including induced vitellogenin production [5,13,14,20], hermaphroditism as evidenced by the presence of both testicular and ovarian tissues [8,10,11,18,31], reduced testis size [20] and reduced plasma androgen concentrations together with delayed sexual maturity [26].Some information is known on the effect of estrogens and estrogenic chemicals on the carp testis, and their relation to seasonal changes in testis morphology. Three-month exposure to a sublethal dose of 4-tert-pentylphenol or 17β-estradiol causes progressive disappearance of spermatozoa and spermatogenic cysts, and reduces the seminiferous tubule diameter, in mature male carp [11]. Ovo-testes were found in juvenile carp exposed to xeno-estrogens during sexual differentiation [8,9]. However, a field study on adult crucian carp (Carassius carassius) living in a pond containing treated pulp mill effluent showed no gonadal deformation or feminization of male gonads [23]. It is therefore important to study the testis of mature male carp (Cyprinus carpio) living in rivers ...

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“…Experimental blood Vtg in male fish collected form natural waters, for example, the river Mississippi [12], the Hudson 2 .1. Chemicals and instruments River at Lake Luzerne, NY, and the Mohawk River at Frankfort, NY [13], the Escambia River in Florida 17b-Estradiol and heparin was obtained from [14], the River Lea in North London and the River Sigma (St. Louis, MO, USA), aprotinin was from Aire in Yorkshire [15], and the Tyne and Mersey Boehringer (Mannheim, Germany), a high-molecuestuaries in the UK [16]. lar-mass calibration kit for SDS electrophoresis was The main techniques for Vtg determination curfrom Pharmacia (Amersham Pharmacia Biotech, rently are radioimmunoassay (RIA), enzyme linked UK), other chemicals used for SDS electrophoresis immunosorbent assay (ELISA) and Western blots were from Sigma, quinaldine sulfate was from the [17].…”

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confidence: 99%

Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma

Shi

Shao

Jiang

et al. 2003

Journal of Chromatography B

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A membrane chromatographic method was developed for the rapid purification of vitellogenin (Vtg) from the plasma of 17b-estradiol induced loach (Misgurnus angaillicaud atus) and carp (Cyprinus carpio). The time required for the proposed procedure is less then 10 min at a flow-rate of 5 ml / min of the mobile phase, and 0.5 ml of fish plasma could be separated in one cycle. Multistep gradient elution was more suitable for the separation than linear gradient elution. Under optimized conditions, a single Vtg peak can be obtained and its identity was confirmed by SDS-PAGE and gel-permeation chromatography assessment. This method is rapid and easy to operate compared to conventional HPLC and FPLC columns for Vtg separation. 

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A Comparison of the Reproductive Physiology of Largemouth Bass, Micropterus salmoides, Collected from the Escambia and Blackwater Rivers in Florida

Cited by 11 publications

References 22 publications

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Gonadosomatic Index and Testis Morphology of Common Carp (Cyprinus carpio) in Rivers Contaminated with Estrogenic Chemicals.

Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma

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