A Comparison of the Microbial Production and Combustion Characteristics of Three Alcohol Biofuels: Ethanol, 1-Butanol, and 1-Octanol

Kremer, Florian; Blank, Lars M.; Jones, Patrik R.; Akhtar, M. Kalim

doi:10.3389/fbioe.2015.00112

Cited by 29 publications

(18 citation statements)

References 49 publications

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“…Fuels derived from various bio-based feedstocks have attracted great attention in recent decades [1][2][3]. Oxygen containing biofuels, such as alcohols, have shown considerable promise, because they are renewable and considered to be neutral with regard to net greenhouse gas emissions [4][5][6][7][8][9][10][11][12][13][14][15]. Octanol has generated a considerable amount of interest where recently new pathways were described to obtain n-octanol from biomass or bio-oil.…”

Section: Introductionmentioning

confidence: 99%

Reactive force field simulation studies on the combustion behavior of n -octanol

Bharti

Banerjee

2016

Fuel Processing Technology

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Section: Introductionmentioning

confidence: 99%

Reactive force field simulation studies on the combustion behavior of n -octanol

Bharti

Banerjee

2016

Fuel Processing Technology

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“…They are considered as complete replacements of fossil fuels or as additives for blending due to similar characteristics regarding critical parameters [ 4 , 5 ]. Among a comprehensive portfolio of approved molecules from microbial production [ 5 – 9 ], 1-octanol has acquired special attention as substitute for diesel and jet fuels [ 10 – 13 ]. Previous studies [ 10 , 14 ] compared various characteristics of fossil-derived as well as bio-derived diesel fuels with saturated short- and medium-chain alcohols, and showed that 1-octanol exhibits best matching overall properties compared to ethanol or other long-chain alcohols.…”

Section: Introductionmentioning

confidence: 99%

An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae

Henritzi

Fischer

Grininger

et al. 2018

Biotechnol Biofuels

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BackgroundThe ideal biofuel should not only be a regenerative fuel from renewable feedstocks, but should also be compatible with the existing fuel distribution infrastructure and with normal car engines. As the so-called drop-in biofuel, the fatty alcohol 1-octanol has been described as a valuable substitute for diesel and jet fuels and has already been produced fermentatively from sugars in small amounts with engineered bacteria via reduction of thioesterase-mediated premature release of octanoic acid from fatty acid synthase or via a reversal of the β-oxidation pathway.ResultsThe previously engineered short-chain acyl-CoA producing yeast Fas1R1834K/Fas2 fatty acid synthase variant was expressed together with carboxylic acid reductase from Mycobacterium marinum and phosphopantetheinyl transferase Sfp from Bacillus subtilis in a Saccharomyces cerevisiae Δfas1 Δfas2 Δfaa2 mutant strain. With the involvement of endogenous thioesterases, alcohol dehydrogenases, and aldehyde reductases, the synthesized octanoyl-CoA was converted to 1-octanol up to a titer of 26.0 mg L−1 in a 72-h fermentation. The additional accumulation of 90 mg L−1 octanoic acid in the medium indicated a bottleneck in 1-octanol production. When octanoic acid was supplied externally to the yeast cells, it could be efficiently converted to 1-octanol indicating that re-uptake of octanoic acid across the plasma membrane is not limiting. Additional overexpression of aldehyde reductase Ahr from Escherichia coli nearly completely prevented accumulation of octanoic acid and increased 1-octanol titers up to 49.5 mg L−1. However, in growth tests concentrations even lower than 50.0 mg L−1 turned out to be inhibitory to yeast growth. In situ extraction in a two-phase fermentation with dodecane as second phase did not improve growth, indicating that 1-octanol acts inhibitive before secretion. Furthermore, 1-octanol production was even reduced, which results from extraction of the intermediate octanoic acid to the organic phase, preventing its re-uptake.ConclusionsBy providing chain length control via an engineered octanoyl-CoA producing fatty acid synthase, we were able to specifically produce 1-octanol with S. cerevisiae. Before metabolic engineering can be used to further increase product titers and yields, strategies must be developed that cope with the toxic effects of 1-octanol on the yeast cells.

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“…Of particular interest are, e.g. substances for the food, feed, and pharma application, as well as platform chemicals from renewable carbon sources for the production of polymeric materials and biofuels . The platform organism Corynebacterium glutamicum is already applied as industrial workhorse for large‐scale biotechnological production processes covering a range of products.…”

Section: Introductionmentioning

confidence: 99%

Plug flow versus stirred tank reactor flow characteristics in two‐compartment scale‐down bioreactor: Setup‐specific influence on the metabolic phenotype and bioprocess performance of Corynebacterium glutamicum

Limberg

Pooth

Wiechert

et al. 2016

Engineering in Life Sciences

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In the last two decades, scale‐down studies based on compartmented reactor setups became the standard procedure to mimic inhomogeneous cultivation conditions. In the academic field and with application to industrial‐scale, two basic scale‐down bioreactor configurations both showing a stirred tank reactor (STR) as main compartment predominate this research field. The connection to a plug flow reactor (PFR) generates oscillatory gradients with a distinct residence time of the culture, while the STR provides a broad residence time distribution leading to more heterogeneous oscillations. The influence of these opposed hydrodynamic profiles for their applicability for scale‐down bioreactor setups as well as their specific influence on the metabolic phenotype of l‐lysine producing Corynebacterium glutamicum DM1800 strain was investigated. Batch cultivations under oscillatory oxygen deprivation and substrate excess were carried out in STR–PFR and STR–STR scale‐down devices. In both setups, the induced inhomogeneity resulted in a reduction of growth rate and increased the l‐lactate and l‐glutamate by‐product formation, while biomass and product yields stayed nearly constant. Apart from differing side‐product levels, very similar results were observed when comparing the metabolic phenotype and bioprocess performance of STR–PFR and STR–STR configuration, although opposed back mixing profiles were present.

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A Comparison of the Microbial Production and Combustion Characteristics of Three Alcohol Biofuels: Ethanol, 1-Butanol, and 1-Octanol

Cited by 29 publications

References 49 publications

Reactive force field simulation studies on the combustion behavior of n -octanol

Reactive force field simulation studies on the combustion behavior of n -octanol

An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae

Plug flow versus stirred tank reactor flow characteristics in two‐compartment scale‐down bioreactor: Setup‐specific influence on the metabolic phenotype and bioprocess performance of Corynebacterium glutamicum

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