A comparison of the effects of a chlamydial vaccine administered during or after a C. muridarum urogenital infection of female mice

Carey, Alison J.; Cunningham, Kyle W.; Andrew, Dean; Hafner, Louise M.; Beagley, Kenneth W.

doi:10.1016/j.vaccine.2011.07.012

Cited by 8 publications

(8 citation statements)

References 37 publications

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“…Plates were washed 3x with PBS containing 0.5% Tween 20 (PBS-T) and blocked with 5% fetal bovine serum in PBS-T at 37 °C for 2 h. ELISAs were performed with serum and vaginal wash samples as previously described [34,41]. MBP-MOMP specific IgG and IgA were detected with 1/1000 HRP- conjugated goat anti-mouse IgG and goat anti-mouse IgA respectively (Southern Biotech, InVitro Technologies, VIC, Australia).…”

Section: Methodsmentioning

confidence: 99%

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The Duration of Chlamydia muridarum Genital Tract Infection and Associated Chronic Pathological Changes Are Reduced in IL-17 Knockout Mice but Protection Is Not Increased Further by Immunization

et al. 2013

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IL-17 is believed to be important for protection against extracellular pathogens, where clearance is dependent on neutrophil recruitment and local activation of epithelial cell defences. However, the role of IL-17 in protection against intracellular pathogens such as Chlamydia is less clear. We have compared (i) the course of natural genital tract C. muridarum infection, (ii) the development of oviduct pathology and (iii) the development of vaccine-induced immunity against infection in wild type (WT) BALB/c and IL-17 knockout mice (IL-17-/-) to determine if IL-17-mediated immunity is implicated in the development of infection-induced pathology and/or protection. Both the magnitude and duration of genital infection was significantly reduced in IL-17-/- mice compared to BALB/c. Similarly, hydrosalpinx was also greatly reduced in IL-17-/- mice and this correlated with reduced neutrophil and macrophage infiltration of oviduct tissues. Matrix metalloproteinase (MMP) 9 and MMP2 were increased in WT oviducts compared to IL-17-/- animals at day 7 post-infection. In contrast, oviducts from IL-17-/- mice contained higher MMP9 and MMP2 at day 21. Infection also elicited higher levels of Chlamydia -neutralizing antibody in serum of IL-17-/- mice than WT mice. Following intranasal immunization with C. muridarum Major Outer Membrane Protein (MOMP) and cholera toxin plus CpG adjuvants, significantly higher levels of chlamydial MOMP-specific IgG and IgA were found in serum and vaginal washes of IL-17-/- mice. T cell proliferation and IFNγ production by splenocytes was greater in WT animals following in vitro re-stimulation, however vaccination was only effective at reducing infection in WT, not IL-17-/- mice. Intranasal or transcutaneous immunization protected WT but not IL-17-/- mice against hydrosalpinx development. Our data show that in the absence of IL-17, the severity of C. muridarum genital infection and associated oviduct pathology are significantly attenuated, however neither infection or pathology can be reduced further by vaccination protocols that effectively protect WT mice.

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Section: Methodsmentioning

confidence: 99%

“…muridarum infection [31] [32] [33,34]. Interestingly, recent studies by Zygmunt et al [35] have shown that the IN route of immunization preferentially induces a Th17 response, further suggesting that IL-17 may have a role in genital tract chlamydial immunity.…”

Section: Introductionmentioning

confidence: 99%

The Duration of Chlamydia muridarum Genital Tract Infection and Associated Chronic Pathological Changes Are Reduced in IL-17 Knockout Mice but Protection Is Not Increased Further by Immunization

et al. 2013

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“…IN immunization with live C. muridarum (Pal et al, 1994), vault nanoparticles (Champion et al, 2009), soluble MOMP combined with cholera toxin, CpG, or CTA1-DD (Skelding et al, 2006;Carey et al, 2011) all reduce infection and/or pathology in mice. Protection was associated with generation of IFNγ-secreting T cells and recruitment of antibody secreting cells to the site of infection (Skelding et al, 2006;Andrew et al, 2011;Tammiruusu et al, 2007).…”

Section: Mucosal Routes Of Vaccine Deliverymentioning

confidence: 99%

Animal Models of Immunity to Female Genital Tract Infections and Vaccine Development

2015

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“…Chlamydia muridarum is a murine pathogen that causes disease similar to that of Chlamydia trachomatis in humans (Carey et al, 2011). As such, it is commonly used as a murine model.…”

Section: Chlamydia and Streptococcusmentioning

confidence: 99%

“…As such, it is commonly used as a murine model. Normally, C. trachomatis manifests in humans as a sexually-transmitted disease, infecting the genital tract (Carey et al, 2011). However, it can infect other sites as well, including the respiratory tract, and, as a result, experiments have been performed using experimental C. muridarum lung infections (He et al, 2011).…”

Section: Chlamydia and Streptococcusmentioning

confidence: 99%

The Contrasting Roles of T Regulatory Cells in Bacterial Lung Diseases

Odeh¹,

Simecka²

2012

Respiratory Diseases

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A comparison of the effects of a chlamydial vaccine administered during or after a C. muridarum urogenital infection of female mice

Cited by 8 publications

References 37 publications

The Duration of Chlamydia muridarum Genital Tract Infection and Associated Chronic Pathological Changes Are Reduced in IL-17 Knockout Mice but Protection Is Not Increased Further by Immunization

The Duration of Chlamydia muridarum Genital Tract Infection and Associated Chronic Pathological Changes Are Reduced in IL-17 Knockout Mice but Protection Is Not Increased Further by Immunization

Animal Models of Immunity to Female Genital Tract Infections and Vaccine Development

The Contrasting Roles of T Regulatory Cells in Bacterial Lung Diseases

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