A comparison of the density of microtubules in the central and peripheral axonal branches of the pseudounipolar neurons of lizard spinal ganglia

Pannese, Ennio; Ledda, M.; Arcidiacono, G.; Rigamonti, L; Procacci, Patrizia

doi:10.1002/ar.1092080415

Cited by 28 publications

(11 citation statements)

References 43 publications

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“…That is, the number of MT does not change in terms of unit of axonal area in small or large axons. It has been previously reported that MT number depends on axonal area (Ochs et al, 1978;Pannese et al, 1984). However, for small axons, those results are consistent with the results reported here.…”

Section: Discussionsupporting

confidence: 94%

Microtubule stability along mammalian peripheral nerves

Donoso

1986

J. Neurobiol.

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Microtubule (MT) number, axonal area, and MT density were examined in unmyelinated axons of rat cervical vagus nerve. Study of nerve regions proximal (1-5 mm) and distal (35-40 mm) to the nodosum ganglion in controls (incubation at 37 degrees C for 1 h) showed that the number of MT per axon is significantly less in distal than in proximal nerve regions. Cooling (incubation at 0 degree C for 1 h) caused a significant reduction in the number of MT per axon in both nerve regions. The unmyelinated axons from both nerve regions showed a comparable reduction in MT number by cooling, indicating that axonal MT stability to cold was not significantly different between these two nerve regions. In these nerves no detectable changes were found in cross-axonal area of unmyelinated axons between distal and proximal nerve regions. In another experimental series, in distal nerve regions (35-40 mm from the nodosum ganglion) the number of MT was not further reduced in nerves incubated at 0 degree C by increasing the incubation time. Similar results were obtained from colchicine treated nerves (incubation at 37 degrees C, with 10 mM colchicine for 1 and 2 h). Distal nerve regions (35-40 mm from the nodosum ganglion) showed a similar reduction in the number of MT per axon when nerves were incubated at 0 degree C or with colchicine, suggesting that this drug, as well as cold, may be affecting a similar population of axonal MT, i.e., MT susceptible to anti-MT agents. These results indicate that approximately one-half of the axonal MT are stable to cold as well as to colchicine in rat unmyelinated axons.

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Section: Discussionsupporting

confidence: 94%

Microtubule stability along mammalian peripheral nerves

Donoso

1986

J. Neurobiol.

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“…In peripheral nerves, in the restricted range where the size of myelinated and nonmedullated axons overlaps, the microtubular content is also the same in both axonal classes (Pannese et al, 1984a;Facndez and Alvarez, 1986). On the other hand, in sensory neurons, radicular and peripheral branches of equal size and class exhibit different microtubular packings, as observed here in the rat and reported for cats and lizards (Fadic et al, 1985;Pannese et al, 1984b;Serra and Alvarez, 1989). All these observations indicate that the low microtubular content of radicular fibres is related to their lying in the root, and unrelated to being sensory or motor, myelinated or nonmedullated.…”

supporting

confidence: 77%

“…This we will call the radicular pattern. This asymmetry about the primary bifurcation of the sensory axon has been observed in toads, lizards and cats (FadiC et al, 1985;Pannese et al, 1984b;Saitua and Alvarez, 1989;Serra and Alvarez, 1989). Since axons of peripheral nerves share a common pattern of microtubular packing, FadiC et al (1985) conjectured that the radicular pattern may be shared with central nervous system (CNS) axons.…”

Section: Introductionmentioning

confidence: 81%

The Microtubular Pattern Changes at the Spinal Cord‐Root Junction and Reverts at the Root‐Peripheral Nerve Junction in Sensory and Motor Fibres of the Rat

López

Álvarez

1990

Eur J of Neuroscience

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In the rat, we studied the microtubular content of central nervous system (CNS) axons (pyramidal tract, dorsal funiculus, and intracord domain of motor axons), of radicular axons (ventral and dorsal roots), and of peripheral axons (sural and lateral gastrocnemius nerves). The microtubular density had an inverse relationship with the size of the axon. Within the CNS, values ranged from over 120 microtubules/microm2 for axons smaller than 0.1 microm2 of the pyramidal tract and dorsal funiculus to 24 for 3-microm motor axons (area, 7 microm2) in their spinal cord domain. Peripheral nerve and CNS axons of the same size had comparable microtubular densities. In contrast, the microtubular density of dorsal and ventral root axons was one half that of CNS or peripheral nerve axons of equal calibre. Considered along the axon, the microtubular density of motor and sensory fibres is high in the CNS domain, low in the root, and high again in the peripheral nerve domain. These observations are inconsistent with the notion that the cytoskeleton moves coherently away from the perikaryon. We conclude that the axonal microtubular content accords with the calibre of the fibre and with the anatomical region where it courses. We propose that axonal microtubules are regulated by local cues.

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“…The second half of the 20th century saw an explosion of publications on lizards and snakes in The Anatomical Record . Topics include effects of controlled temperature and day length on gonadal development (Bartholomew, ), gross and microscopic anatomy of the liver and gall bladder (Ells, ), osteology and musculature (Evans, ; Bellairs and Bryant, ; Throckmorton, ; Throckmorton and Saubert, ; Rubolini et al, ; Hall, ; Payne et al, ; Direnzo and Stynoski, ), endocrine and reproductive biology (Miller, ; Fox, ; Cavazos and Feagans, ; Neaves, ; Jacobs and Sis, ; Rhoten and Hall, ; Rhoten, ; Jones et al, ; Jones and Summers, ; Moscona, ; Young et al, ), and neurobiology (Zika and Singer, ; Colborn and Adamo, ; Proske, ; Gundy and Wurst, ; Baird and Lowman, ; Sligar and Voneida, ; Yeager et al, ; Pannese et al, ; McDevitt et al, ; Amemiya et al, ). Among the endocrinology papers are three reports by long‐time American Association of Anatomists' member William B. Rhoten, whose research focused on the pancreas of the garter snake (Rhoten and Hall, ; Rhoten, ).…”

mentioning

confidence: 99%