A comparison of the contractile properties of smooth muscle from pig urethra and internal anal sphincter

Ramalingam, T.; Durlu-Kandilci, Nezahat Tugba; Brading, Alison F.

doi:10.1002/nau.20863

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Due to the small size of urethral ring used in experiments, we did not separate smooth muscle from striated muscle of urethra sphincter. So the contractile response of the smooth muscle component can not be completely excluded in this study; even though we applied the EFS with low frequency and duration (1 Hz and 0.2 ms) that have been shown to evoke very low contractile response in smooth muscle . Third, due to lack of molecular production and pathological study, a detailed discussion on the potential mechanisms should be limited.…”

Section: Discussionmentioning

confidence: 99%

Impaired contractility of the circular striated urethral sphincter muscle may contribute to stress urinary incontinence in female zucker fatty rats

Lin

Wang

Reed‐Maldonado

et al. 2016

Neurourology and Urodynamics

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AIM Obesity has been an independent risk factor for female stress urinary incontinence (SUI), the mechanism of this association remains unknown. The aim of this study is to validate the hypothesis that urethral dysfunction is a possible contributor to SUI in obese women. METHODS Ten Zucker Fatty (ZF) (ZUC-Leprfa 185) and 10 Zucker Lean (ZL) (ZUC-Leprfa 186) female rats at 12-week-old were used in this experiment. The urethral sphincter rings were harvested from the bladder neck through to the most proximal 2/3 regions. In the organ bath study, single pulses of electrical field stimulation (EFS) were applied. For the fatiguing stimulation, repeated multi-pulse EFS with 70 mA were applied at frequency of 5 Hz for 5 min. Caffeine-containing Krebs’ solution was administrated to contract the urethra until the contraction began to reach a plateau for 10 min. We performed immunofluorescence staining of the urethra after the experiment was finished. RESULTS Compared to ZL controls, ZF rats had significantly impaired muscle contractile activity (MCA) (P < 0.05). Also, ZF rats presented early fatiguing of MCA and had a significantly greater percentage of MCA decline from baseline in the fatiguing test (37.7% vs 25.6%, P < 0.05). The plateau of maximal MCA induced by caffeine in ZF rats was significantly lower than ZL controls (0.22 vs 0.36, P < 0.05). CONCLUSIONS This novel study showed that obese female rats had significantly impaired contractile properties of striated urethral sphincter, suggesting urethral dysfunction could be an important contributor to SUI in obesity.

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Section: Discussionmentioning

confidence: 99%

Impaired contractility of the circular striated urethral sphincter muscle may contribute to stress urinary incontinence in female zucker fatty rats

Lin

Wang

Reed‐Maldonado

et al. 2016

Neurourology and Urodynamics

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“…Organ bath experiment was used for functional evaluation of contractile urethra and blood vessel ex vivo for the contractility study [12, 13]; female pig urethra and blood vessel were prepared from the basal part of the bladder body and renal artery, respectively [13]. Urethra and renal artery were mounted between platinum-plate electrodes and secured by small clips in a double-jacketed organ bath containing 20 mL Krebs' solution aerated with 95% O 2 and 5% CO 2 to obtain a pH of 7.4 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Treatment of Stress Urinary Incontinence by Cinnamaldehyde, the Major Constituent of the Chinese Medicinal Herb Ramulus Cinnamomi

Chen

Lin

Chen

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Stress urinary incontinence (SUI) is a common disorder in middle-aged women and the elderly population. Although surgical treatment of SUI has progressed, pharmacological therapies remain unelucidated. We screened potential herbal medicines against SUI with an ex vivo organ bath assay. Ramulus Cinnamomi and its major constituent cinnamaldehyde cause a high contractile force of the urethra and a low contractile force of blood vessels. Cinnamaldehyde dose-dependently reduced lipopolysaccharide-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. In the vaginal distension- (VD-) induced SUI model in mice, cinnamaldehyde significantly reversed the VD-induced SUI physical signs and reduced blood pressure. Cinnamaldehyde may offer therapeutic potential against SUI without the possible side effect of hypertension. The modulation of several SUI-related proteins including myosin, iNOS, survival motor neuron (SMN) protein, and superoxide dismutase 3 (SOD3) may play some crucial roles in the therapeutic approach against SUI. This information may offer clues to the pathogenesis of SUI and open additional avenues for potential therapy strategies.

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Anatomical basis of the coordination between smooth and striated urethral and anal sphincters: loops of regulation between inferior hypogastric plexus and pudendal nerve. Immuno-histological study with 3D reconstruction

et al. 2016

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A comparison of the contractile properties of smooth muscle from pig urethra and internal anal sphincter

Abstract: There are differences between urethra and IAS in terms of muscarinic activation and neural innervation, relevant for pharmacotherapy.

Cited by 4 publications

References 17 publications

Impaired contractility of the circular striated urethral sphincter muscle may contribute to stress urinary incontinence in female zucker fatty rats

Impaired contractility of the circular striated urethral sphincter muscle may contribute to stress urinary incontinence in female zucker fatty rats

Treatment of Stress Urinary Incontinence by Cinnamaldehyde, the Major Constituent of the Chinese Medicinal Herb Ramulus Cinnamomi

Anatomical basis of the coordination between smooth and striated urethral and anal sphincters: loops of regulation between inferior hypogastric plexus and pudendal nerve. Immuno-histological study with 3D reconstruction

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