Anatomical basis of the coordination between smooth and striated urethral and anal sphincters: loops of regulation between inferior hypogastric plexus and pudendal nerve. Immuno-histological study with 3D reconstruction

Bertrand, Martin; Alsaid, Bayan; Droupy, S.; Ripoche, J.; Galand, Benoît; Adalian, Pascal; Brunet, Christian; Piercecchi-Marti, M.D.; Prudhomme, Michel

doi:10.1007/s00276-016-1655-4

Cited by 9 publications

(10 citation statements)

References 31 publications

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“…These methods may underestimate the preganglionic axons as these proteins are usually present at higher levels in axon terminals rather than axon tracts, and nitric oxide synthase may not be expressed by all preganglionic neurons. To our knowledge, sensory markers have not been examined in the adult or fetal pelvic splanchnic nerves, although they have been reported in the bladder and rectum projection from the fetal inferior hypogastric plexus (Bertrand et al 2016). All of these observations in human tissues (adults or foetuses) should be interpreted with caution because of small sample size and the high level of anatomical variation in the primary structures of this part of the nervous system (Schlyvitsch and Kosintzev, 1939).…”

Section: Discussionmentioning

confidence: 99%

Functional segregation within the pelvic nerve of male rats: a meso‐ and microscopic analysis

et al. 2020

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The pelvic splanchnic nerves are essential for pelvic organ function and have been proposed as targets for neuromodulation. We have focused on the rodent homologue of these nerves, the pelvic nerves. Our goal was to define within the pelvic nerve the projections of organ‐specific sensory axons labelled by microinjection of neural tracer (cholera toxin, subunit B) into the bladder, urethra or rectum. We also examined the location of peptidergic sensory axons within the pelvic nerves to determine whether they aggregated separately from sacral preganglionic and paravertebral sympathetic postganglionic axons travelling in the same nerve. To address these aims, microscopy was performed on the major pelvic ganglion (MPG) with attached pelvic nerves, microdissected from young adult male Sprague–Dawley rats (6–8 weeks old) and processed as whole mounts for fluorescence immunohistochemistry. The pelvic nerves were typically composed of five discrete fascicles. Each fascicle contained peptidergic sensory, cholinergic preganglionic and noradrenergic postganglionic axons. Sensory axons innervating the lower urinary tract (LUT) consistently projected in specific fascicles within the pelvic nerves, whereas sensory axons innervating the rectum projected in a complementary group of fascicles. These discrete aggregations of organ‐specific sensory projections could be followed along the full length of the pelvic nerves. From the junction of the pelvic nerve with the MPG, sensory axons immunoreactive for calcitonin gene‐related peptide (CGRP) showed several distinct patterns of projection: some projected directly to the cavernous nerve, others projected directly across the surface of the MPG to the accessory nerves and a third class entered the MPG, encircling specific cholinergic neurons projecting to the LUT. A subpopulation of preganglionic inputs to noradrenergic MPG neurons also showed CGRP immunoreactivity. Together, these studies reveal new molecular and structural features of the pelvic nerves and suggest functional targets of sensory nerves in the MPG. These anatomical data will facilitate the design of experimental bioengineering strategies to specifically modulate each axon class.

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Section: Discussionmentioning

confidence: 99%

Functional segregation within the pelvic nerve of male rats: a meso‐ and microscopic analysis

et al. 2020

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“…Most authors further specified the intervals between serial sections, with a variable array of intervals reported across the studies. These distances included defined ranges (e.g., 5 μm thick sections every 50–150 μm (e.g., Alsaid et al, 2011)), “variable intervals” that were not specified (e.g., Bertrand et al, 2016), serial sections without intervals (e.g., Zaitouna et al, 2013), or negligible intervals noted to be 5 μm (e.g., Bertrand et al, 2013). Karam et al (2005) described using 200 μm intervals, as this fell within the capacity of the 3D software utilized to adequately compare anatomical structures.…”

Section: Resultsmentioning

confidence: 99%

“…These regions included: the bony pelvis, pelvic organs, vasculature, musculature, fascia, connective tissue, and ligaments (Table 1). Many studies reported that the rationale for reconstructing innervation of the female pelvis was to inform specific surgical procedures to reduce iatrogenic outcomes (Alsaid et al, 2011; Bertrand et al, 2013; Bertrand et al, 2016; Bertrand, Macri, et al, 2014; Kalfa et al, 2008; Kraima et al, 2016; Li, Duan, et al, 2019; Moszkowicz et al, 2012; Moszkowicz, Alsaid, Bessede, Penna, et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…While we were able to describe a common workflow that can generally be used to create such 3D images and models, our detailed review also identified some variability across the reported 3D reconstruction methodologies. For example, there was a notable inconsistency in reported distances between serial sections across the IHC‐based studies reviewed (Alsaid et al, 2011; Bertrand et al, 2013; Bertrand et al, 2016; Karam et al, 2005; Zaitouna et al, 2013). It is unclear why the authors chose the various sectioning distances and whether these parameters could influence the accuracy of the 3D model generated, or the ability to compare anatomical features across the generated images.…”

Section: Discussionmentioning

confidence: 99%

“…One of the benefits of using IHC for 3D reconstruction is the ability to use a variety of markers that can identify different fiber types (e.g., autonomic vs. sensory). Identification of nerve fibers and the types of fibers can provide clinicians with vital information on the location of nerves, which can be used to prevent unwanted damage to nerves involved in functions such as pain perception, tactile sensation, gland stimulation, and proper organ function (Bertrand et al, 2016; Stjernholm et al, 2000). Such techniques have successfully been used to identify a protective sheath surrounding the inferior hypogastric plexus, within the lateral pelvic wall, and thus have provided evidence for the optimal areas of incision for nerve preservation during total mesorectal excision (Bertrand et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Three‐dimensional reconstruction of the innervation of the female pelvis: A review of current methods

et al. 2023

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The three‐dimensional (3D) reconstruction of tissues is a valuable approach for elucidating the anatomy of nerves and plexuses, which are often microscopic in nature and therefore difficult to fully appreciate using gross dissection approaches alone. A common workflow which can be used to generate such 3D models has yet to be comprehensively described. This study aimed to review 3D reconstruction methodologies and findings related to human female pelvic innervation to determine whether there is an optimal methodology and identify the limitations of these approaches. A comprehensive literature review was conducted using keywords including 3D reconstruction, human female pelvic nerves, and innervation. Twenty relevant articles published between 2003 and 2019 were selected for review. The 3D reconstruction of female pelvic innervation generally follows two workflows involving either immunohistochemistry (IHC) (n = 16) or magnetic resonance imaging (MRI) (n = 4). There were commonalities among the general steps reported for 3D tissue reconstruction across these two imaging methodologies. Notably, there was some variability in study methodology across the studies reviewed, suggesting there is not a clear best practice for the reconstruction of these tissues. Information that generates 3D mapping of innervation has important clinical applications, such as informing and optimizing surgical approaches to avoid damage to local innervation. IHC and MRI‐based approaches are both feasible for the reconstruction of pelvic innervation, though there are advantages and disadvantages to both. Information from this review can be used to help inform the development of 3D models of female pelvic innervation in the future.

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Surgical Treatment of Deep Endometriosis

Fernandes

Afors

Wattiez

2018

Minimally Invasive Gynecology

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Anatomical basis of the coordination between smooth and striated urethral and anal sphincters: loops of regulation between inferior hypogastric plexus and pudendal nerve. Immuno-histological study with 3D reconstruction

Cited by 9 publications

References 31 publications

Functional segregation within the pelvic nerve of male rats: a meso‐ and microscopic analysis

Functional segregation within the pelvic nerve of male rats: a meso‐ and microscopic analysis

Three‐dimensional reconstruction of the innervation of the female pelvis: A review of current methods

Surgical Treatment of Deep Endometriosis

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