A comparison of the chromatographic properties of silica gel and silicon hydride modified silica gels

Sutcliffe, Oliver B.; Euerby, Melvin R.; Bawazeer, Sami; Watson, David

doi:10.1016/j.chroma.2012.09.005

Cited by 21 publications

(12 citation statements)

References 21 publications

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“…In particular, the elution of peptides 3, 4, 5, and 6 could not be achieved within a reasonable time frame until the amount of water was increased to at least 35% v/v in the mobile phase. Recently, Bawazeer et al compared the retention properties of silica and silica hydride phases for bases, sugars, and polar acids using FA or ammonium acetate as mobile phase additives, and found that the silica hydride phases was more retentive than the silica phase for most compounds investigated [32]. Nevertheless, in the present study, the peptide mixture could not be well resolved under the isocratic elution conditions as peptide 1 and 2 coeluted, while peptides 3, 4, 5, and 6 were only partially separated from each other.…”

Section: Resultsmentioning

confidence: 99%

Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

et al. 2013

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The retention behavior of a set of polar peptides separated on a silica hydride stationary phase was examined with a capillary HPLC system coupled to ESI-MS detection. The mobile phases consisted of formic acid or acetic acid/acetonitrile/water mixtures with the acetonitrile content ranging from 5 to 80% v/v. The effects on peptide retention of these two acidic buffer additives and their concentrations in the mobile phase were systematically investigated. Strong retention of the peptides on the silica hydride phase was observed with relatively high-organic low-aqueous mobile phases (i.e. under aqueous normal-phase conditions). However, when low concentrations of acetic acid were employed as the buffer additive, strong retention of the peptides was also observed even when high aqueous content mobile phases were employed. This unique feature of the stationary phase therefore provides an opportunity for chromatographic analysis of polar peptides with water-rich eluents, a feature usually not feasible with traditional RP sorbents, and thus under conditions more compatible with analytical green chemistry criteria. In addition, both isocratic and gradient elution procedures can be employed to optimize peptide separations with excellent reproducibility and resolution under these high aqueous mobile phase conditions with this silica hydride stationary phase.

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Section: Resultsmentioning

confidence: 99%

Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

et al. 2013

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“…Watson et al . reported on the chromatographic behavior of various polar metabolites on a Cogent Diamond™ hydride column in aqueous‐organic mobile phases (pH 6.5) and concluded−without explicitly referring to the hydrolytic stability of the LC phase−that the hydride material differs on its chromatographic performance from bare silica . Our own results (see Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 71%

“…These results seem to contradict those of Jandera and coworkers who, based on linear structure-energy relationship and Langmuirian models, apparently assumed longterm stability of various hydride-based bonded silicas in a number of water-rich mobile phases (pH 3.2 and at elevated temperatures) to explain the retention behavior of selected polar solutes [26][27][28]. Watson et al reported on the chromatographic behavior of various polar metabolites on a Cogent Diamond TM hydride column in aqueous-organic mobile phases (pH 6.5) and concluded−without explicitly referring to the hydrolytic stability of the LC phase−that the hydride material differs on its chromatographic performance from bare silica [29]. Our own results (see Supporting Information Fig.…”

Section: Silica Hydride Stability a Subject Of Controversymentioning

confidence: 99%

Novel 3‐hydroxypropyl‐bonded phase by direct hydrosilylation of allyl alcohol on amorphous hydride silica

2014

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A novel 3-hydroxypropyl (propanol) bonded silica phase has been prepared by hydrosilylation of allyl alcohol on a hydride silica intermediate, in the presence of platinum (0)-divinyltetramethyldisiloxane (Karstedt's catalyst). The regio-selectivity of this synthetic approach had been correctly predicted by previous reports involving octakis(dimethylsiloxy)octasilsesquioxane (Q8M8H) and hydrogen silsesquioxane (T8H8), as molecular analogs of hydride amorphous silica. Thus, C-silylation predominated (~ 94%) over O-silylation, and high surface coverages of propanol groups (5±1 µmol/m2) were typically obtained in this work. The propanol-bonded phase was characterized by spectroscopic (IR and solid state NMR on silica microparticles), contact angle (on fused-silica wafers) and CE (on fused-silica tubes) techniques. CE studies of the migration behavior of pyridine, caffeine, tris(2,2’-bipyridine)Ru(II) chloride and lysozyme on propanol-modified capillaries were carried out. The adsorption properties of these select silanol-sensitive solutes were compared to those on the unmodified and hydride-modified tubes. It was found that hydrolysis of the SiH species underlying the immobilized propanol moieties leads mainly to strong ion-exchange based interactions with the basic solutes at pH 4, particularly with lysozyme. Interestingly, and in agreement with water contact angle and electroosmotic mobility figures, the silanol-probe interactions on the buffer-exposed (hydrolyzed) hydride surface are quite different from those of the original unmodified tube.

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“…Chromatographic conditions such as stationary phase (column), mobile phase composition (solvent ratio, pH, salt type, its concentration), and temperature have significant effects on the chromatography behavior of polar compounds under HILIC . Type B silica (Atlantis HILIC Silica) and type C silica (Cogent Silica‐C column) columns were investigated to compare the retention of ME because it has been found previously that type silica C showed higher retention than type B silica column, particularly for basic compounds .…”

Section: Resultsmentioning

confidence: 99%

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Douša

Pivoňková

Sýkora

2016

J of Separation Science

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A rapid procedure for the determination of memantine based on hydrophilic interaction chromatography with fluorescence detection was developed. Fluorescence detection after postcolumn derivatization with o-phtaldialdehyde/2-mercaptoethanol was performed at excitation and emission wavelengths of 345 and 450 nm, respectively. The postcolumn reaction conditions such as reaction temperature, derivatization reagent flow rate, and reagents concentration were studied due to steric hindrance of amino group of memantine. The derivatization reaction was applied for the hydrophilic interaction liquid chromatography method which was based on Cogent Silica-C stationary phase with a mobile phase consisting of a mixture of 10 mmol/L citric acid and 10 mmol/L o-phosphoric acid (pH 6.0) with acetonitrile using an isocratic composition of 2:8 v/v. The benefit of the reported approach consists in a simple sample pretreatment and a quick and sensitive hydrophilic interaction chromatography method. The developed method was validated in terms of linearity, accuracy, precision, and selectivity according to the International Conference on Harmonisation guidelines. The developed method was successfully applied for the analysis of commercial memantine tablets.

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A comparison of the chromatographic properties of silica gel and silicon hydride modified silica gels

Cited by 21 publications

References 21 publications

Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

Analysis of polar peptides using a silica hydride column and high aqueous content mobile phases

Novel 3‐hydroxypropyl‐bonded phase by direct hydrosilylation of allyl alcohol on amorphous hydride silica

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

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