Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Abstract

A rapid procedure for the determination of memantine based on hydrophilic interaction chromatography with fluorescence detection was developed. Fluorescence detection after postcolumn derivatization with o-phtaldialdehyde/2-mercaptoethanol was performed at excitation and emission wavelengths of 345 and 450 nm, respectively. The postcolumn reaction conditions such as reaction temperature, derivatization reagent flow rate, and reagents concentration were studied due to steric hindrance of amino group of memantine. The derivatization reaction was applied for the hydrophilic interaction liquid chromatography method which was based on Cogent Silica-C stationary phase with a mobile phase consisting of a mixture of 10 mmol/L citric acid and 10 mmol/L o-phosphoric acid (pH 6.0) with acetonitrile using an isocratic composition of 2:8 v/v. The benefit of the reported approach consists in a simple sample pretreatment and a quick and sensitive hydrophilic interaction chromatography method. The developed method was validated in terms of linearity, accuracy, precision, and selectivity according to the International Conference on Harmonisation guidelines. The developed method was successfully applied for the analysis of commercial memantine tablets.

Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o‐phthaldialdehyde/mercaptoethanol

“…The influence of flow rate of derivatization reagent , the derivatization reagent concentration and pH , temperature of derivatization reaction , and finally the volume of capillary coil reactor on the fluorescence detector response have been investigated for the conditions of postcolumn derivatization. The influence of the molecular structure on detector response during derivatization reaction of amines with OPA has already been shortly discussed .…”

“…In the literature, it was shown that the application of postcolumn derivatization is more suitable due to simplicity and instability of the OPA fluorescent derivatives . The influence of flow rate of derivatization reagent , the derivatization reagent concentration , buffer pH , temperature of derivatization reaction , and finally the volume of capillary coil reactor on detector response have been investigated for the conditions of postcolumn derivatization.…”

“…In the literature, it was shown that the application of postcolumn derivatization is more suitable due to simplicity and instability of the OPA fluorescent derivatives [3]. The influence of flow rate of derivatization reagent [3,4], the derivatization reagent concentration [5,6], buffer pH [3], temperature of derivatization reaction [5], and finally the volume of capillary coil reactor [3] on detector response have been investigated for the conditions of postcolumn derivatization.Analysis of volatile amines is difficult because of their high volatility, polarity, basic character, and lack of How to cite this article: Douša M, Doubský J. Separation of structurally related primary aliphatic amines using hydrophilic interaction chromatography with fluorescence detection after postcolumn derivatization with o-phthaldialdehyde/mercaptoethanol.…”

Recent applications of hydrophilic interaction liquid chromatography in pharmaceutical analysis

“…Following that, according to the difference kind of the detected targets, HILIC applied for chemical drug analysis in formulation and related matrices is summarized. Determination of APIs in complicated formulation is important for the QC of chemical drug in pharmaceutical industry, and various related researches have been proposed through HILIC coupled detection system [83][84][85][86]. In powder for intravenous injection, ␤-lactam antibiotics imipenem, and another active compound cilastatin sodium were determined simultaneously in the presence of degradation product thienamycin [83].…”

3D spongy-like Au film for highly stable solid contact potentiometric ion selective electrode: application to drug analysis

“…Direct UV absorption and fluorescence analysis of memantine is challenging due to absence of chromophoric groups and the weak UV absorbance of the primary amine group. Derivatization reactions were proposed to impart measurable characteristics to mematine to enable UV, fluorescence and liquid chromatographic quantification [2][3][4][5][6][7][8][9][10]. Unfortunately, however, derivatization reactions involve highly reactive compounds that require high precautions and special safety measures.…”