A comparison of techniques for size measurement of nanoparticles in cell culture medium

Abstract

Four common size analysis techniques were applied to engineered silica nanoparticles suspended in purified water, in physiological buffer and in cell culture medium, and the results were compared using uncertainty estimates.

Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties

“…SAXS offered highly precise values while CLS yielded detailed size distributions from which further information on the agglomeration state can be derived. 542 Minelli and co-workers used tunable resistive pulse sensing (TRPS), DCS and DLS to measure the size of silica NPs in serum. Also in this case, DLS precision was not sufficient because of the presence of agglomerates.…”

Measuring the size and density of nanoparticles by centrifugal sedimentation and flotation

“…For the bottom of the capillary, a high density mixture was prepared with a fructose (Sigma-Aldrich, Missouri, USA) mass fraction of 67% and a corresponding solvent electron density of 433 nm À3 , while on the top side of the capillary a low density preparation with the same volume fraction of sample (17%) was introduced, using an ethanol mass fraction of 78.5% with a solvent electron density of 280 nm À3 . The scattering experiments were performed with the SAXS setup of Helmholtz-Zentrum Berlin 25 at the four-crystal monochromator beamline of PTB 19 at the synchrotron radiation facility BESSY II (Helmholtz-Zentrum Berlin, Germany) with a photon energy of E ¼ (8000.0 AE 0.8) eV. By means of the continuous contrast variation technique described elsewhere 20 scattering patterns at different suspending medium electron densities were collected during 60 s at a distance L ¼ (4539 AE 1) mm from the capillaries using a vacuum-compatible Pilatus 1 M hybrid pixel detector (Dectris Ltd, Baden, Switzerland) with a pixel size of d ¼ (172.1 AE 0.2) mm.…”

Establishing SI-Traceability of Nanoparticle Size Values Measured with Line-Start Incremental Centrifugal Liquid Sedimentation

“…Line-start centrifugal liquid sedimentation (CLS), also known as differential or disc centrifugal sedimentation or disc centrifuge photosedimentometry (hence the abbreviation used in this article: disc-CLS), is a widely used technique for the characterization of nanoparticles. Its popularity is mainly due to the relatively low capital and operational costs (compared to, for example, electron microscopy) and the ultra-high size resolving power that allows distinguishing, for example, populations of individual particles from populations of small agglomerated particles [2]. To obtain an effective separation of the particles according to their size and effective (buoyant) density, the disc cavity is partly filled with a carrier fluid that has a radial density (and viscosity) gradient.…”

“…Overview of key parameters (first replicate) for the direct calculation of Stokes diameter results using the reference disc-CLS method. Light extinction-weighted modal Stokes diameter 2. At a temperature of 30.0 • C (ERM-FD102) and 27.8 • C (ERM-FD304) 3.…”

“…Light extinction-weighted modal Stokes diameter 2. At a temperature of 30.0 °C (ERM-FD102) and 27.8 °C (ERM-FD304) 3.…”