A comparison of Rubazyme-M and MACRIA for the detection of rubella-specific IgM

Best, Jennifer M.; Palmer, Sara J.; Morgan‐Capner, P.; Hodgson, Julian

doi:10.1016/0166-0934(84)90044-2

Cited by 29 publications

(13 citation statements)

References 7 publications

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“…Rubella IgM assays have been evaluated previously, but not recently (Arista et al, 1987;Best et al, 1984;Cubie and Edmond, 1985;Echevarria et al, 1985;Enders and Knotek, 1986;Hodgson and Morgan-Capner, 1984;Hudson and Morgon-Capner, 1996;Matter et al, 1994;Revello et al, 1987). Many of those assays previously evaluated are either not currently commercially available or have since been modified.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of rubella IgM enzyme immunoassays

Tipples

Hamkar

Mohktari-Azad

et al. 2004

Journal of Clinical Virology

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Section: Discussionmentioning

confidence: 97%

Evaluation of rubella IgM enzyme immunoassays

Tipples

Hamkar

Mohktari-Azad

et al. 2004

Journal of Clinical Virology

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“…Patients who have been persistently reactive for rubella-specific IgM at reasonable concentrations have been described infrequently [5,6], although persistent low concentrations have been described after immunization [17]. The four cases we describe were all investigated after contact with a case of presumed rubella.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, cases have been reported [5,6] in which specific IgM has persisted for many months or longer, but such persistence only becomes apparent when the patient has been serologically followed over that duration. When the patient first presents, it is easy to misinterpret the detection of specific IgM in such a patient as indicating recent rubella.…”

Section: Introductionmentioning

confidence: 99%

The avidity of specific IgM detected in primary rubella and reinfection

Thomas

Morgan‐Capner

1990

Epidemiol. Infect.

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SUMMARYAn IgM capture enzyme-linked immunosorbent assay for rubella-specific IgM was used to assess the avidity of specific IgM by comparing the results obtained with and without a mild protein denaturant in the washing fluid used after incubation of IgM with rubella haemagglutinating antigen. An avidity index (Al) was calculated with AIs < 50% considered to indicate low avidity. Sera from recent primary rubella, rubella reinfection and from patients persistently reactive for specific IgM were tested. Urea and diethylamine (DEA) were compared as the protein denaturants. Twenty-six of 28 sera from cases of primary rubella gave an Al < 50% with DEA, compared with 25 of 28 with urea. Seventeen of 20 sera from cases of reinfection gave an Al > 50 % with DEA whereas only 14 of 20 had a similarly high avidity with urua. Eight of 10 sera from 4 cases of persistent specific IgM reactivity gave AIs > 50 % with DEA, although this was reduced to 5 when urea was used. Thus a difference has been demonstrated between the avidity of specific IgM in primary infection from that demonstrated after a secondary antigenic challenge (reinfection). This may help in serologically distinguishing primary infection from reinfection.

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“…It has been discussed by Best and Banatvala (1987) and Best and O'Shea (1988). False positive and false negative results must be avoided, and the laboratory procedures used must be tuned to the highest possible specificity and sensitivity.…”

Section: Serologic Assessment Of Pregnant Womenmentioning

confidence: 97%

“…Rubella vaccines induce an immune response in about 95% of susceptible vaccinees. Immune re-sponses have been discussed in more detail by Best and O'Shea (1988). Vaccines produce minimal reactions in children, but arthropathy may develop in adults between 10 days and 4 weeks after vaccination (Banatvala, 1977;Perkins, 1985).…”

Section: Preventionmentioning

confidence: 98%

Togaviridae: Rubella Virus

Best¹,

O’Shea²

1988

Laboratory Diagnosis of Infectious Diseases Principles and Practice

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Disease: Rubella, congenital rubella. Etiologic Agent: Rubella virus, genus Rubivirus of Togaviridae. Clinical Manifestations: Asyptomatic infections are common; fever with rash; lymphadenitis; arthropathy and arthritis in adults. Congenital infection may result in fetal death and spontaneous abortion, live birth of severely malformed infant, an infant with minimal damage, or a healthy infant. Pathology: Primary site of virus replication is the nasopharyngeal mucosa, followed by spread of virus to local lymph nodes and a viremia 7 days after infection. The appearance of a rash may be caused by viral antigen-antibody complexes. During pregnancy, the virus infects the placenta and is then disseminated to all fetal tissues. Laboratory Diagnosis: IgG serology in paired serum specimens; IgM serology in a single serum. IgG serology is in large-scale use for immunity testing. Epidemiology: Worldwide distribution; endemic in temperate climates, with seasonal peaks during spring and early summer. In countries with successful childhood vaccination programs, major epidemics have been prevented.

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A comparison of Rubazyme-M and MACRIA for the detection of rubella-specific IgM

Cited by 29 publications

References 7 publications

Evaluation of rubella IgM enzyme immunoassays

Evaluation of rubella IgM enzyme immunoassays

The avidity of specific IgM detected in primary rubella and reinfection

Togaviridae: Rubella Virus

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