Evaluation of rubella IgM enzyme immunoassays

Tipples, Graham; Hamkar, Rasool; Mohktari-Azad, Talat; Gray, Michael J.; Ball, Jennifer; Head, Carol; Ratnam, Samuel

doi:10.1016/j.jcv.2003.11.006

Cited by 61 publications

(45 citation statements)

References 25 publications

(22 reference statements)

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“…Different viruses such as measles, human parvovirus B19, and enterovirus cause symptoms similar to those of rubella (2,25,32). Also, misclassification of cases of CRS may occur, since some patients with CRS may have a single symptom or defect or may have a positive result for immunoglobulin M (IgM) enzyme immunosorbent assays (EIAs) without clinical symptoms (4,5,33).…”

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confidence: 99%

Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription-Loop-Mediated Isothermal Amplification

et al. 2006

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confidence: 99%

Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription-Loop-Mediated Isothermal Amplification

et al. 2006

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“…The selection of a diagnostic test depends on different parameters consisting of high sensitivity, high specificity, low price, simple procedure, less time consuming and adaptability to automation (14). qRT-PCR was performed for virus titration would be expected to be a reliable and rapid diagnostic method in clinical applications, because it is known to be superior for detection of viral copies without virus isolation from samples.…”

Section: Discussionmentioning

confidence: 99%

“…Serological tests like the hemagglutination inhibition method are performed basically with paired sera from only the acute and convalescent phases (12). Although, IgM-based ELISA is employed for diagnosis of rubella using a single serum sample, but false-positive results are frequently occurres similar to other virological tests (13)(14)(15). As well, plaque assay was given a precise estimation of lived RV.…”

Section: Introductionmentioning

confidence: 99%

Development of qRT-PCR Test for Quantification of Rubella Virus in Commercially Available Vaccines

Sheikholeslami

Khanahmadi²,

Ajorloo

et al. 2015

Iran J Virol

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Background and Aims: Determination of virions in live vaccines, especially multivalent vaccines is considered as a critical point and in-process of quality control (IPQC) test of vaccine production. Rapid tests, like real time PCR, are more appropriate when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. In the current study, a real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was developed for Rubella virus (RV) and tested on a commercially available MMR vaccine. Materials and Methods:The primers and Taq Man probe were designed by Gene runner version 5.0.63 software. Concentrations, as well as reaction temperatures were optimized to establish an efficient qRT-PCR assay for RV RNA. A RV-specific PCR amplicon (109 bp), conserved in all species, was made as an external standard to evaluate the linearity, amplification efficiency, analytical sensitivity and reproducibility of the qRT-PCR. Results:The real time quantitative assay was shown to have good linearity (R 2 =0.998), high amplification efficiency (E=96.18%), and high sensitivity (7 × 10 2 copies/7 µl) for tested vaccine. Conclusion:The established qRT-PCR method is a simple, rapid, quantitative, highly specific and sensitive assay for quantification of RV RNA copy numbers in IPQC tests at industrial scale.

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“…Consideration must be given, however, to the possibility of false-positive rubella virus IgM results where the prevalence of rubella is low (2,15). Also, IgM may persist beyond the expected period of 4 weeks (14).…”

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confidence: 99%

“…The low-avidity panel consisted of 49 sera collected from acute rubella cases as previously described (15). The high-avidity panel consisted of 53 sera from pregnant women undergoing routine prenatal rubella screening.…”

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confidence: 99%

Evaluation of Commercial Rubella Immunoglobulin G Avidity Assays

2007

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We compared the performances of five commercial rubella virus immunoglobulin G (IgG) avidity assays. The Adaltis (kappa ‫؍‬ 0.28) and Diesse (kappa ‫؍‬ 0.33) assays showed poor correlation, the Behring assay (kappa ‫؍‬ 0.68) showed good correlation, and the Euroimmun (kappa ‫؍‬ 0.95) and Radim (kappa ‫؍‬ 0.94) assays showed excellent correlation with a well-established in-house rubella virus IgG avidity assay. The Euroimmun and Radim assays were statistically significantly better than the other commercial assays (P < 0.01).

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Evaluation of rubella IgM enzyme immunoassays

Cited by 61 publications

References 25 publications

Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription-Loop-Mediated Isothermal Amplification

Development of a New Method for Diagnosis of Rubella Virus Infection by Reverse Transcription-Loop-Mediated Isothermal Amplification

Development of qRT-PCR Test for Quantification of Rubella Virus in Commercially Available Vaccines

Evaluation of Commercial Rubella Immunoglobulin G Avidity Assays

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