A comparison of peripheral blood stem cell apheresis using the fenwal CS3000 plus and COBE spectra

Dzieczkowski, Jeffery S.; McGonigal, Marilyn; Cook, Jane K.A.; Sugrue, Michele W.; Andersen, Janet; Anderson, Kenneth C.

doi:10.1016/0955-3886(94)00061-n

Cited by 21 publications

(21 citation statements)

References 15 publications

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“…These disadvantages are eliminated with the introduction of the AutoPBSC. In fact, the platelet loss, platelet contamination, and granulocyte contamination, results obtained with the AutoPBSC in our study are similar, if not superior, to those obtained with the CS 3000, 31,32 the AS104, 30 and the MCS‐3P 29 …”

Section: Discussionsupporting

confidence: 83%

Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Ravagnani¹,

Siena²,

Reys³

et al. 1999

Transfusion

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Section: Discussionsupporting

confidence: 83%

Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Ravagnani¹,

Siena²,

Reys³

et al. 1999

Transfusion

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“…Even though our results confirm this study (we find approximately the same extraction factor per unit of blood volume), we find that, in order to collect the same amount of cells harvested in 3 h with the Spectra, the patients should undergo 10 h of leukapheresis with the MCS-3P. Although this study was performed with a particular continous flow cell separator, others have demonstrated that the same results are obtainable with continous flow cell separators from other companies [7]. …”

Section: Discussionsupporting

confidence: 62%

“…The calculation we applied for comparing the two separators (extraction ratio) is based on the proportion per unit time between the absolute number of extracted cells and the calculated number of pre-procedure circulating cells in the patients’ whole blood; this differs from other studies in which the extraction efficiency was calculated per patient’s weight or per unit of processed blood volume [6, 7]. The extraction ratio represents a more direct and exact way to compare the efficiency of the extraction procedure.…”

Section: Discussionmentioning

confidence: 86%

Intrapatient Comparison of an Intermittent and a Continous& ;Flow Cell Separator for the Collection of Progenitor and Stem Cells from the Blood

Beretta¹,

Bosch²,

Castelli³

et al. 1998

Vox Sanguinis

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Background and Objectives: Continuous-flow and intermittent-flow blood cell separators (CFCS and IFCS) are both used to collect stem cells from the blood to rescue patients undergoing myeloablative treatment for cancer. Materials and Methods: We designed a study to compare the collection efficiency of the two systems. The continous-flow Cobe Spectra and the intermittent-flow Haemonetics MCS-3P were used to collect cells on consecutive days from 9 patients mobilised with G-CSF with or without chemotherapy. Blood obtained before leukapheresis and the leukapheresis product were analysed for their content of red and white cells, platelets, CD34-positive cells, GM-CFC, CFC-E, and BFU-E. An extraction ratio was calculated. Results: We found that the CFCS extracted about 4 times more mononuclear cells per unit time, 3 times more CD34-positive, and 4 times more clonogenic cells than the IFCS. The subject acceptability of the two systems was similar. Conclusion: The CFCS is a more efficient system for stem cell collection. IFCS requires a longer harvesting time for the same result.

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“…Other reports described CD34-CE between 48 and 87% at preapheresis peripheral blood CD34+ cell counts of approximately 30/µl, using the cell separators Fresenius AS104, Haemonetics MCS-3P, COBE Spectra (protocol 4.7) and/or Fenwal CS3000 [9, 10, 11, 19, 20, 21]. Some authors reported better CD34-CE at a preapheresis level of circulating CD34+ cells <50/µl [13, 22].…”

Section: Discussionmentioning

confidence: 99%

“…The collection of PBSC can be performed on various cell separator devices operating with semiautomated or automated apheresis programs [9, 10, 11]. The aim of the present study was to evaluate collection yields of conventional-volume COBE Spectra AutoPBSC leukaphereses and to demonstrate clinical significance of collected PBSC following HDCT.…”

Section: Discussionmentioning

confidence: 99%

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

et al. 2000

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Background: A convenient, effective and safe peripheral blood stem cell (PBSC) apheresis procedure is desirable to cope with the increasing requirements for PBSC collections. We performed PBSC harvesting with the novel COBE Spectra AutoPBSC^TM system using the default software configuration recommended by the manufacturer. We analyzed collection parameters and clinical efficiency of harvested autografts following high-dose chemotherapy (HDCT). Patients and Methods: Eighty-one patients underwent 102 harvests after standard chemotherapy plus filgrastim (5–10 μg/kg/day) to obtain a target of ≥2.5 × 10⁶ CD34+ cells/kg for autologous blood stem cell transplantation. Conventional-volume leukaphereses (median: 11 liters) were performed using the manufacturer’s standard software default regarding inlet flow, harvest/chase volume (3/7 ml) and number of collection cycles. The ratio of ACD-A to whole blood was initially set at 1:12 (56 collections), later at 1:10 (46 aphereses). Results: With respect to preapheresis counts of 93 (9–876) CD34+ cells/μl, 69 patients (85.2%) achieved ≥2.5 × 10⁶ CD34+ cells/kg by the first apheresis. PBSC products contained medians of 5.0 × 10⁶ (0.7–77.3) CD34+ cells/kg and 13.8 × 10⁴ (2.3–105.0) CFU-GM/kg. A preapheresis count of ≥40 CD34+ cells/μl predicted a single-apheresis yield of ≥2.5 × 10⁶ CD34+ cells/kg. Apheresis products showed a high mononuclear cell (MNC) purity of ≥89%. The median overall collection efficiency of CD34+ cells (CD34-CE) was 42.6% (12.2–87.4). The CD34-CE decreased significantly with increasing numbers of circulating CD34+ cells: 52.5% at CD34+ cells <40/μl versus 41.0% at CD34+ cells ≥40/μl (p ≤ 0.002). Coagulation problems occurred in 3 of 56 procedures at an ACD-A to whole blood ratio of 1:12, but in no case at 1:10. Patients who underwent HDCT and autologous blood stem cell transplantation (n = 40) engrafted in a median time of 9 (8–13) days for absolute neutrophil counts >0.5 × 10³/μl, 10 (8–13) days for WBC >1.0 × 10³/μl and 11 (8–17) days for platelets >20 × 10³/μl. Conclusions: As a result of efficient PBSC mobilization, a single conventional-volume leukapheresis with the COBE Spectra AutoPBSC system resulted in hematopoietic autografts with ≥2.5 × 10⁶ CD34+ cells/kg in 85% of patients. Following the standard PBSC apheresis recommendations of the manufacturer, the AutoPBSC system assures PBSC products with a high MNC purity and a moderate CD34-CE that declines significantly at increasing levels of circulating CD34+ cells. Leukaphereses performed at an ACD-A to whole blood ratio of 1:10 should run without coagulation problems.

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A comparison of peripheral blood stem cell apheresis using the fenwal CS3000 plus and COBE spectra

Cited by 21 publications

References 15 publications

Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Intrapatient Comparison of an Intermittent and a Continous& ;Flow Cell Separator for the Collection of Progenitor and Stem Cells from the Blood

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

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