A Comparison of Methods for Determining Total Body Protein

Brooks, Stephen P. J.; Lampi, B.; Sarwar, Ghulam; Botting, H. G.

doi:10.1006/abio.1995.1186

Cited by 46 publications

(23 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipid content was determined by the method of Folch et al 21 and converted to energy as lipid by using the coefficient of 39.2 kJ=g. Protein content was determined as described by Brooks et al 22 and converted to energy as protein by using the value of 23.5 kJ=g.…”

Section: Body Composition Measurementsmentioning

confidence: 99%

Skeletal muscle oxidative capacity in rats fed high-fat diet

et al. 2002

View full text Add to dashboard Cite

OBJECTIVE: To investigate whether young rats respond to high-fat feeding through changes in energy efficiency and fuel partitioning at the level of skeletal muscle, to avoid obesity development. In addition, to establish whether the two mitochondrial subpopulations, which exist in skeletal muscle, ie subsarcolemmal and intermyofibrillar, are differently affected by high-fat feeding. DESIGN: Weaning rats were fed a low-fat or a high-fat diet for 15 days. MEASUREMENTS: Energy balance and lipid partitioning in the whole animal. State 3 and state 4 oxygen consumption rates in whole skeletal muscle homogenate. State 3 and state 4 oxygen consumption rates, membrane potential and uncoupling effect of palmitate in subsarcolemmal and intermyofibrillar mitochondria from skeletal muscle. RESULTS: Rats fed a high-fat diet showed an increased whole body lipid utilization. Skeletal muscle NAD-linked and lipid oxidative capacity significantly increased at the whole-tissue level, due to an increase in lipid oxidative capacity in subsarcolemmal and intermyofibrillar mitochondria and in NAD-linked activity only in intermyofibrillar ones. In addition, rats fed a highfat diet showed an increase in the uncoupling effect of palmitate in both the mitochondrial populations. CONCLUSIONS: In young rats fed a high-fat diet, skeletal muscle contributes to enhanced whole body lipid oxidation through an increased mitochondrial capacity to use lipids as metabolic fuels, associated with a decrease in energy coupling.

show abstract

Section: Body Composition Measurementsmentioning

confidence: 99%

Skeletal muscle oxidative capacity in rats fed high-fat diet

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Lipid content was determined gravimetrically after extraction in chloroform ± methanol and evaporation to constant weight by a rotating evaporator (Heidolh, Germany) by the method of Folch et al 20 The energy as lipid was calculated from the lipid content using the coef®cient of 39.2 kJag for the energy content of lipid. Protein content was measured using the Biuret method on samples of carcass homogenate treated with 5% SDS plus 0.5 M NaOH, as outlined by Brooks et al 21 The value of 22.4 kJag was used for the energy content of protein. Water content was determined by the difference in weight of the homogenate before and after drying at 70 C in a vacuum oven.…”

Section: Measurement Of Body Compositionmentioning

confidence: 99%

Fat balance and serum leptin concentrations in normal, hypothyroid, and hyperthyroid rats

et al. 2001

View full text Add to dashboard Cite

OBJECTIVE:To study the in¯uence of thyroid hormones on the relationship between serum leptin and fat mass, as well as on energy and macronutrient balance. DESIGN: Rats with different thyroid states were obtained by 7 and 15 days of treatment with the antithyroid drug propylthiouracil or with triiodothyronine (T 3 ). MEASUREMENTS: Energy balance, macronutrient balance and serum leptin concentrations. RESULTS: In hypothyroid rats we found a decrease in metabolizable energy (ME) intake and energy expenditure together with an increase in lipid gainalipid intake ratio and a decrease in protein gainaprotein intake ratio. Consequently, body lipid percentage signi®cantly increased compared to euthyroid rats. Hyperthyroid rats ®rst increased energy expenditure and later ME intake, so that increased metabolism was balanced by increased intake, and energy gain was similar to that found in euthyroid rats. CONCLUSION: These results indicate that T 3 plays a major role in the maintenance of energy and lipid balance. Our results also indicate that an inverse relationship exists between T 3 and leptin serum concentrations, and that this relationship is not only the result of changes in body fat stores induced by changed T 3 concentrations.

show abstract

“…Lipid content was determined gravimetrically after extraction in chloroform±methanol and evaporation to constant weight by a rotating evaporator (Heidolh, Germany) by the method of Folch et al 24 The energy as lipid was calculated from the lipid content by using the coef®-cient of 39.2 kJag for the energy content of lipid. Protein content was determined by the Biuret method after extraction in SDS-NaOH as described by Brooks et al 25 The energy as protein was calculated from the protein content by using the value of 23.5 kJag for the energy content of protein. Water content was determined by the difference in weight of the homogenate before and after drying at 70 C in a vacuum oven.…”

Section: Body Composition Measurementsmentioning

confidence: 99%

Fat balance and hepatic mitochondrial function in response to fat feeding in mature rats

et al. 1999

View full text Add to dashboard Cite

OBJECTIVE: To study the effects of fat feeding on fat balance and hepatic mitochondrial function in postpubertal male rats. DESIGN: Rats were fed low fat, medium fat or high fat diet for 15 days. MEASUREMENTS: Energy balance, body composition, resting metabolic rate (RMR), mitochondrial state 3 and state 4 oxygen consumption rates, succinic dehydrogenase (EC 1.3.99.1) and mitochondrial a-glycerophosphate dehydrogenase (EC 1.1.1.8) activities. RESULTS: Rats fed medium fat or high fat diet, in comparison with rats fed low fat diet, showed a signi®cantly greater metabolisable energy intake and energy expenditure. In addition, body energy and lipid gains were signi®cantly higher in rats fed medium fat or high fat diet than in rats fed low fat diet. Mitochondrial respiration and enzymatic activities were not affected by fat feeding. CONCLUSION: These results indicate that in postpubertal rats fed high fat diets, the increase in energy expenditure counteracts only in part the excess fat deposition. This is probably due to the impairment in regulatory responses, and enhances thermogenesis.

show abstract

A Comparison of Methods for Determining Total Body Protein

Cited by 46 publications

References 0 publications

Skeletal muscle oxidative capacity in rats fed high-fat diet

Skeletal muscle oxidative capacity in rats fed high-fat diet

Fat balance and serum leptin concentrations in normal, hypothyroid, and hyperthyroid rats

Fat balance and hepatic mitochondrial function in response to fat feeding in mature rats

Contact Info

Product

Resources

About