A comparison of intradermal test with recombinant tuberculosis allergen (diaskintest) with other immunologic tests in the diagnosis of tuberculosis infection

Старшинова, Анна; Zhuravlev, Viacheslav; Dovgaluk, Irina; Пантелеев, А. М.; Манина, В. В.; Zinchenko, Ulia; Istomina, Evgenia; Pavlova, Maria; Yаblonskiy, Petr

doi:10.4103/ijmy.ijmy_17_18

Cited by 29 publications

(25 citation statements)

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“…Therefore, the probability of identifying active TB in patients with positive Diaskintest results was over 70% (PPV), which is quite high. The data correspond to those reported in other studies, in which the PPV of Diaskintest for identifying active TB was between 63% and 84% in adults and children (Slogotskaya et al, 2011;Shovkun et al, 2015;Losovskaya et al, 2016;Starshinova et al, 2018). The present analysis of QFT results showed a comparable outcome, while according to the data of other authors, the PPV of QFT ranges from 65% to 86% (Shovkun et al, 2015;Losovskaya et al, 2016;Starshinova et al, 2018).…”

Section: Discussionsupporting

confidence: 91%

“…Diaskintest has been compared to the TST and demonstrated a significantly higher specificity, apparently due to the use of M. tuberculosis-specific antigens not reacting with BCG or non-tuberculous mycobacteria (Slogotskaya et al, 2011(Slogotskaya et al, , 2017(Slogotskaya et al, , 2018. However, publications on the comparative performances of Diaskintest and IGRAs are limited in number (Slogotskaya et al, 2012;Losovskaya et al, 2016;Starshinova et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Comparative performance of QuantiFERON-TB Gold versus skin test with tuberculosis recombinant allergen (Diaskintest) among patients with suspected pulmonary tuberculosis in Russia

Nikitina

Karpina

Kasimceva

et al. 2019

International Journal of Infectious Diseases

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Background: The early identification of Mycobacterium tuberculosis infection can prevent tuberculosis (TB) transmission. A skin test with a tuberculosis recombinant allergen (Diaskintest) is a new method for identification that has been implemented in Russia. This study was performed to compare the performances of Diaskintest and QuantiFERON-TB Gold (QFT) in adults and children with suspected TB in Moscow, Russia. Methods: Adults (n = 85) and children (n = 96) were tested using Diaskintest and QFT. Concordance and comparative analyses were performed. Results: Diaskintest and QFT were concordant in 84% of adults and 90% of children (overall concordance 87%, k > 0.6, K c > 0.5). The concordance between QFT, Diaskintest, and the final diagnosis was good in adults (86% and 81%, respectively) and moderate in children (77% and 79%, respectively). In adults, QFT had a higher sensitivity for detecting TB than Diaskintest (82% and 68%, respectively); in children, Diaskintest was more sensitive (73% and 65%, respectively). In patients with a confirmed TB diagnosis, negative Diaskintest/QFT results were associated with low disease activity. Combined Diaskintest/QFT results identified TB patients with higher sensitivity and specificity than each test separately. Conclusions: Diaskintest is a low-cost diagnostic tool that shows a test positivity rate similar to QFT and can be used in combination with QFT as an adjunctive test for TB diagnosis. (O.V. Kasimceva), hergertv@mail.ru (V.Y. Gergert), cniit@ctri.ru (A. Ergeshov), ivlyadova@mail.ru (I.V. Lyadova).

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Comparative performance of QuantiFERON-TB Gold versus skin test with tuberculosis recombinant allergen (Diaskintest) among patients with suspected pulmonary tuberculosis in Russia

Nikitina

Karpina

Kasimceva

et al. 2019

International Journal of Infectious Diseases

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“…PPD is a precipitate of species-nonspecific molecules from filtrates of sterilized tuberculoid organism cultures and many varieties of PPD are actually in use. Outside of North America, PPD RT23 is the most widely used PPD product, but PPD RT23 Mexico is used in Latin America, PPD-s is used in Japan and PPD-L is used in Russia (Comstock et al 1964 ; Kimura et al 2005 ; Rangel-Frausto et al 2001 ; Starshinova et al 2018 ; Yang et al 2012 ). PPD-S2, the current US standard that was developed to address the eventual depletion of PPD-S, is used in the commercially available Aplisol ® and Tubersol ® (Jensen et al 2005 ; Villarino et al 2000 ).…”

Section: Discussionmentioning

confidence: 99%

The single recombinant M. tuberculosis protein DPPD provides enhanced performance of skin testing among HIV-infected tuberculosis patients

et al. 2020

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Diagnostic testing for M. tuberculosis infection has advanced with QuantiFERON and GeneXpert, but simple costeffective alternatives for widespread TB screening has remained elusive and purified protein derivative (PPD)-based tuberculin skin testing (TST) remains the most widely used method. PPD-based tests have reduced performance, however, in BCG vaccinees and in individuals with immune deficiencies. We compared the performance of skin testing with the recombinant DPPD protein against that of a standard PPD-based skin test. Our data indicates similar performance of DPPD and PPD (r 2 = 0.7689) among HIV-negative, active TB patients, all of whom presented greater than 10 mm induration following administration. In contrast to results demonstrating that PPD induced indurations greater than 5 mm (i.e., the recommended threshold for positive results in this population) in only half (19 of 38) of the HIV positive TB patients, 89.5% (34 of 38) of these participants developed indurations greater than 5 mm when challenged with DPPD. Importantly, none of the patients that were positive following PPD administration were negative following DPPD administration, indicating markedly improved sensitivity of DPPD among HIV-infected individuals. Our data indicate that DPPD has superior performance in skin testing than the current TST standard.

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“…В настоящей работе установлена высокая чувствительность кожных проб (проба Манту, проба с АТР) и лабораторного теста Т-СПОТ.ТБ (100%). Эти результаты соотносятся с ранее опубликованными данными других авторов [7,9,10].…”

Section: обсуждение полученных данныхunclassified

Modern immunological tests for early detection of tuberculosis infection in children in the Yaroslavl Oblast

Astafiev

Takhkhan

Vasilyeva

et al. 2023

Tihookeanskij medicinskij žurnal

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Objective. To evaluate the efficacy of immunological tests for early detection of tuberculosis infection in children in the Yaroslavl Oblast (Russia).Material and methods. Medical records of 354 children and adolescents examined by ELISPOT (Enzyme-Linked ImmunoSpot) assay (T-SPOT.TB) in the Yaroslavl Oblast in 2020–2022 were studied. Four groups of children were distinguished: group 1 included children with active tuberculosis (n = 3); group 2 included children under 7 with altered tuberculin sensitivity (conversion of the tuberculin test) or children over 8 first-time tested positive for RTA, who were under the supervision of a phthisiatrician in the VI A group of dispensary registration (n = 52); group 3 included children with medical exemptions from screening immunodiagnostics (n = 49); and group 4 included children with refusals of their parents or legal representatives from skin tests (n = 250).Results. The sensitivity of both RTA and T-SPOT.TB tests achieved 100%, with the concordance level of 100%. When assessing discordant results (positive result for RTA and negative for T-SPOT.TB) in the VI A dispensary registration group, the majority of children were found to have an aggravated allergic anamnesis and somatic pathology. The maximum number of children examined by the laboratory method included those whose parents or legal representatives refused from skin test administration (70.6%).Conclusions. T-SPOT.TB is an optimal method for early detection of latent TB infection and TB in children at risk, including those with medical contraindications for skin tests or whose parents refused from screening immunodiagnostics.

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A comparison of intradermal test with recombinant tuberculosis allergen (diaskintest) with other immunologic tests in the diagnosis of tuberculosis infection

Cited by 29 publications

References 0 publications

Comparative performance of QuantiFERON-TB Gold versus skin test with tuberculosis recombinant allergen (Diaskintest) among patients with suspected pulmonary tuberculosis in Russia

Comparative performance of QuantiFERON-TB Gold versus skin test with tuberculosis recombinant allergen (Diaskintest) among patients with suspected pulmonary tuberculosis in Russia

The single recombinant M. tuberculosis protein DPPD provides enhanced performance of skin testing among HIV-infected tuberculosis patients

Modern immunological tests for early detection of tuberculosis infection in children in the Yaroslavl Oblast

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